A modular electrochemical peptide-based sensor for antibody detection

Puiu, Mihaela; Idili, Andrea; Moscone, Danila; Ricci, Francesco; Bala, Camelia

doi:10.1039/c4cc02858a

Cited by 43 publications

(29 citation statements)

References 33 publications

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“…Upon binding with the target, the motional freedom of the peptide probe was hindered, and a detectable reduction in the electrochemical current was observed. Essentially, this approach seems to be versatile, and can be utilized for many other target molecules, including diagnostically relevant antibodies 134,135. An interesting concept, based on recognition tunneling, was recently presented by Zhao and coworkers.…”

Section: Discussionmentioning

confidence: 99%

Predictive Models for Thermal Behavior of Chemicals with Quantitative Structure‐Property Relationships

Baati

Nanchen²,

Stoessel³

et al. 2015

Chem Eng & Technol

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Most processes in the chemical industry involve potentially hazardous steps. It is thus of critical importance to perform risk assessments and to know the thermal behavior of the chemicals at stake. A widely used thermal analysis, differential scanning calorimetry, allows verifying if the compounds are stable towards heat or if they decompose above certain temperatures. This information helps setting the appropriate handling and storage conditions for safe operations. The time and resources needed for these experimental investigations would be reduced if the testing phase could be better targeted and guided using reliable predictive methods. This work helps to answer these needs by proposing predictive models for thermal stability based on the quantitative structure‐property relationships method.

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Section: Discussionmentioning

confidence: 99%

Predictive Models for Thermal Behavior of Chemicals with Quantitative Structure‐Property Relationships

Baati

Nanchen²,

Stoessel³

et al. 2015

Chem Eng & Technol

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show abstract

“…In another study, a modular electrochemical peptide-based sensor was developed to detect anti-DGP antibody [39]. In this approach, firstly a short helical support peptide (SP) was immobilized on the surface of a gold electrode, followed by functionalization of SP with DGP and methylene blue (MB), which are used as the antigen and electrochemical tag, respectively.…”

Section: Research Development For Point-of-care Testsmentioning

confidence: 99%

Challenges with Point-Of-Care Tests (POCT) for Celiac Disease

Wu¹,

Wallach²,

Shimoni³

2019

Celiac Disease - From the Bench to the Clinic

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Current screening test for celiac disease involves blood test in centralized pathology laboratories, typically performing enzyme-linked immune-sorbent assays (ELISA) to detect specific celiac disease antibodies. Most of the current available celiac disease antibody tests detect anti-gliadin (AGA), anti-endomysial (EMA), anti-transglutaminase (tTG), or deamidated gluten peptide (DGP) antibodies from serum or whole blood samples. It requires blood collection from untreated celiac patients, which is often invasive and inconvenient. There is a rapid growth in demand for noninvasive celiac tests for the early and fast diagnosis of celiac disease to help potential celiac patients obtain results and take corresponding actions. Over the last decade, several point-of-care tests (POCT) have been introduced to the market, but these tests have not been widely accepted by clinicians. Moreover, the 2009 NICE guideline CG 86 recommended that self-tests and/or POCT for celiac disease should not be used as a substitute for laboratory-based tests. Here, we provide a background on the evolution of POCT for celiac disease. We discuss general principle of operation for the known commercial kits as well as the use of various antigens and antibodies in different tests developed over the years. Finally, we discuss challenges for future research directions in celiac disease POCTs.

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“…Nonelectroactive peptides can be successfully combined with impedance/voltammetric transducers [14,15]. Furthermore, peptides can be easily labelled with redox-active probes, such as ferrocene (Fc) or methylene blue [16][17][18] for signal-on/signal-off detection. Selected applications in clinical diagnostics, in which these kinds of detection strategies are exploited, are discussed in the following sections.…”

Section: Introductionmentioning

confidence: 99%

The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

et al. 2021

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Peptides represent a promising class of biorecognition elements that can be coupled to electrochemical transducers. The benefits lie mainly in their stability and selectivity toward a target analyte. Furthermore, they can be synthesized rather easily and modified with specific functional groups, thus making them suitable for the development of novel architectures for biosensing platforms, as well as alternative labelling tools. Peptides have also been proposed as antibiofouling agents. Indeed, biofouling caused by the accumulation of biomolecules on electrode surfaces is one of the major issues and challenges to be addressed in the practical application of electrochemical biosensors. In this review, we summarise trends from the last three years in the design and development of electrochemical biosensors using synthetic peptides. The different roles of peptides in the design of electrochemical biosensors are described. The main procedures of selection and synthesis are discussed. Selected applications in clinical diagnostics are also described.

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A modular electrochemical peptide-based sensor for antibody detection

Cited by 43 publications

References 33 publications

Predictive Models for Thermal Behavior of Chemicals with Quantitative Structure‐Property Relationships

Predictive Models for Thermal Behavior of Chemicals with Quantitative Structure‐Property Relationships

Challenges with Point-Of-Care Tests (POCT) for Celiac Disease

The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

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