A molecular analysis of three amelogenin negative males in two routine paternity tests

Zehethofer, Karl; Rolf, Burkhard

doi:10.1016/j.fsigen.2010.04.006

Cited by 10 publications

(1 citation statement)

References 19 publications

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“…Limitations of sex informative tests used in routine forensic analyses are mainly connected with low-quantity and low-quality samples. However, when high DNA-yielding bones are tested with the amelogenin test it can happened that the Y target does not amplify due to mutations or the presence of null alleles [58][59][60][61] , and analyses of additional sex informative markers can solve this problem.…”

Section: Discussionmentioning

confidence: 99%

Genetic sexing of subadult skeletal remains

Zupanič Pajnič,

Mlinšek,

Počivavšek

et al. 2023

Sci Rep

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When subadult skeletons need to be identified, biological sex diagnosis is one of the first steps in the identification process. Sex assessment of subadults using morphological features is unreliable, and molecular genetic methods were applied in this study. Eighty-three ancient skeletons were used as models for poorly preserved DNA. Three sex-informative markers on the Y and X chromosome were used for sex identification: a qPCR test using the PowerQuant Y target included in PowerQuant System (Promega), the amelogenin test included in ESI 17 Fast STR kit (Promega), and a Y-STR amplification test using the PowerPlex Y-23 kit (Promega). Sex was successfully determined in all but five skeletons. Successful PowerQuant Y-target, Y-amelogenin, and Y-chromosomal STR amplifications proved the presence of male DNA in 35 skeletons, and in 43 subadults female sex was established. No match was found between the genetic profiles of subadult skeletons, and the elimination database and negative control samples produced no profiles, indicating no contamination issue. Our study shows that genetic sex identification is a very successful approach for biological sexing of subadult skeletons whose sex cannot be assessed by anthropological methods. The results of this study are applicable for badly preserved subadult skeletons from routine forensic casework.

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Section: Discussionmentioning

confidence: 99%