A molecular and parasitological survey on cutaneous leishmaniasis patients from historical city of Kashan in Isfahan province, center of Iran

Shiee, Mohammad Reza; Hajjaran, Homa; Mohebali, Mehdi; Doroodgar, Abbas; saadat, Masoomeh Hashemi; Teimouri, Aref; Afzali, Hasan; Shirzadi, Mohammad Reza

doi:10.1016/s2222-1808(12)60093-0

Cited by 20 publications

(13 citation statements)

References 16 publications

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“…The results of study showed that percentage of dry type are equale to wet type. This result was disaggrement with the results of (19)(20)(21)(22)(23) who mentioned that wet type more finding than the dry type.…”

Section: (40)supporting

confidence: 69%

Epidemiological Study of Cutaneous Leishmaniasis in Wasit Province

Abdulsada A. Rahi,

Zainab K. Hashim,

Ali Mohsin Al-Jamea

2021

Indian Journal of Forensic Medicine & Toxicology

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The present study was conducted to detect the cutaneous Leishmaniasis from a total of suspected cases that were collected from dermatology center of AL-Karamah Teaching Hospital in Al-Kut city from 1 st October 2019 to the end of February 2020. Totally 60 patients were included 36 males and 24 females. The collected skin specimens were smeared and stained with Giemsa stain, then examined by microscope under oil immersion lenses. The results of current study appeared that 56(93.3%) were positive amastigotes, The prevalence of CL were 36 (60%) in males and 24(40%) in females, while the highest infection 36(60%) in rural area and lowest 24(40%) in urban area. The percent of infection with Cutaneous leishmaniasis according to type of lesions recorded were equal in dry and wet ulcers type (50 % ) for each one.

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Section: (40)supporting

confidence: 69%

Epidemiological Study of Cutaneous Leishmaniasis in Wasit Province

Abdulsada A. Rahi,

Zainab K. Hashim,

Ali Mohsin Al-Jamea

2021

Indian Journal of Forensic Medicine & Toxicology

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“…L. infantum, the principal agent of VL, causes splenomegaly and hepatomegaly [ 3 ]. Most of ACL are reported from northeastern and central parts of Iran [ 4 – 6 ], while ZCL extends from northeast to center and west of Iran and covers all southern provinces as well [ 7 – 9 ]. More than 90% of the visceral cases in Iran are reported from the northwest and some southern provinces including Fars and Bushehr [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification and Polymorphism Determination of Cutaneous and Visceral Leishmaniasis Agents Isolated from Human and Animal Hosts in Iran

Hajjaran

Mohebali

Mamishi

et al. 2013

BioMed Research International

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Amplification of internal transcript spacer 1 of ribosomal RNA (ITS1-RNA) gene followed by RFLP analysis and sequencing was used to identify the causing agents of cutaneous and visceral leishmaniasis (CL and VL) in humans and animal reservoir hosts from various geographical areas in Iran. We also used random amplified polymorphic DNA (RAPD-PCR) to obtain polymorphisms among isolates of Leishmania spp. Totally, 362 suspected human and animal cases including 173 CL, 49 VL, 60 rodents, and 80 domestic dogs were examined for Leishmania infection. From 112 culture-positive samples prepared from CL cases, 75 (67%) were infected with L. major and 37 (33%) with L. tropica. Of the 60 rodents examined, 25 (41.6%) harbored the Leishmania infection; 21 were infected with L. major and 4 with L. turanica. From 49 suspected VL, 29 were positive by direct agglutination test (DAT), whereas microscopy detected parasite in bone marrow of 25 and culture in 28 of the patients. Two VL patients were infected with L. tropica and 26 with L. infantum. Of the 80 domestic dogs, 56 showed anti-Leishmania antibodies with DAT. Of these, 55 were positive by both microscopy and culture. Molecular identity, obtained only for 47 samples, revealed L. infantum in 43 and L. tropica in 4 dogs. The polymorphisms among L. tropica and L. major isolates were 3.6% and 7.3%; the rate among human and canine VL isolates was 2.8% and 9.8%, respectively. Our results showed that at least four different Leishmania species with various polymorphisms circulate among humans and animal hosts in Iran.

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“…major , L . tropica kDNA [ 109 ] 2010 Shiraz Smears, Culture, PCR L. major , L. tropica kDNA [ 49 ] 2010 Kermanshah Culture, PCR-RAPD 6 L. major ITS1 [ 31 ] 2011 Isfahan, Bam PCR-RFLP L. major , L. tropica ITS1 [ 35 ] 2011 All endemic areas Smears, PCR L. major , L. tropica kDNA [ 131 ] 2011 Khuzestan province PCR-RFLP L. major , L. tropica Mini-exon gene [ 137 ] 2012 Kashan Culture, PCR-RFLP L. major , L. tropica ITS1, KDNA [ 17 ] 2012 ...…”

Section: Resultsmentioning

confidence: 99%

Cutaneous leishmaniasis in Iran: A review of epidemiological aspects, with emphasis on molecular findings

et al. 2022

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Leishmania parasites can cause zoonotic cutaneous leishmaniasis (CL) by circulating between humans, rodents, and sandflies in Iran. In this study, published data were collected from scientific sources such as Web of Science, Scopus, PubMed, Springer, ResearchGate, Wiley Online, Ovid, Ebsco, Cochrane Library, Google scholar, and SID. Keywords searched in the articles, theses, and abstracts from 1983 to 2021 were cutaneous leishmaniasis, epidemiology, reservoir, vector, climatic factors, identification, and Iran. This review revealed that CL was prevalent in the west of Iran, while the center and south of Iran were also involved in recent years. The lack of facilities in suburban regions was an aggravating factor in the human community. Some parts of southern Iran were prominent foci of CL due the presence of potential rodent hosts in these regions. Rhombomys opimus, Meriones lybicus, and Tatera indica were well-documented species for hosting the Leishmania species in Iran. Moreover, R. opimus has been found with a coinfection of Leishmania major and L. turanica from the northeast and center of Iran. Mashhad, Kerman, Yazd, and sometimes Shiraz and Tehran foci were distinct areas for L. tropica. Molecular identifications using genomic diagnosis of kDNA and ITS1 fragments of the parasite indicated that there is heterogeneity in leishmaniasis in different parts of the country. Although cutaneous leishmaniasis has been a predicament for the health system, it is relatively under control in Iran.

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A molecular and parasitological survey on cutaneous leishmaniasis patients from historical city of Kashan in Isfahan province, center of Iran

Cited by 20 publications

References 16 publications

Epidemiological Study of Cutaneous Leishmaniasis in Wasit Province

Epidemiological Study of Cutaneous Leishmaniasis in Wasit Province

Molecular Identification and Polymorphism Determination of Cutaneous and Visceral Leishmaniasis Agents Isolated from Human and Animal Hosts in Iran

Cutaneous leishmaniasis in Iran: A review of epidemiological aspects, with emphasis on molecular findings

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