A molecular beacon-assisted nanotheranostic agent for real-time detection and effective combined management of acquired resistance to EGFR-TKIs

Boron Dipyrromethene-Based Nanotheranostic System for Sonophotoassisted Therapy and Simultaneous Monitoring of Tumor Immune Microenvironment Reprogramming

“…After the treatments, cells were imaged by a confocal laser scanning microscope (λ ex : 638 nm; λ em : 650−700 nm) according to our previously reported method. 55 The intracellular distribution was also observed by a confocal laser scanning microscope. 4T1 cells seeded in a 12-well plate were incubated with A-BDP, C-BDP, and CANPs at a concentration of 5 μM C-BDP or 2.5 μM A-BDP for 8 h at 37 °C.…”

“…4T1 and RAW 264.7 cells were seeded into a 12-well plate with 1 mL of culture medium at a density of 5 × 10 4 cells per well and cultured for 12 h. Then, cells were incubated with A-BDP, C-BDP and CANPs at a concentration of 5 μM C-BDP or 2.5 μM A-BDP for different time intervals (2, 4, 6, and 8 h) at 37 °C. After the treatments, cells were collected and subjected to flow cytometric analysis (Beckman CytoFLEX, China) according to our previously reported method …”

“…4T1 cells were seeded in 12-well plates and incubated with A-BDP, C-BDP, and CANPs at a concentration of 5 μM C-BDP or 2.5 μM A-BDP for different time intervals (2, 4, 6, and 8 h) at 37 °C. After the treatments, cells were imaged by a confocal laser scanning microscope (λ ex : 638 nm; λ em : 650–700 nm) according to our previously reported method …”

Yttrium Functionalized Reduced Graphene Oxide Nanocomposite-Based Aptasensor for Ultrasensitive Detection of a Breast Cancer Biomarker

“…4,5 Overexpression of EGFR receptors and excessive levels of EGFR antigen have been associated with cancer; therefore increasing research studies have been conducted to detect EGFR in cancer patients' serum samples. 6,7 Enzymelinked immunosorbent assay, 8 PCR, 9 electron paramagnetic resonance spectrometry, 10 hydrophilic interaction liquid chromatography/mass spectrometry, 11 surface plasmon reso-nance, 12,13 and electrochemical voltammetry methods 6 are some of the analytical techniques currently used to detect EGFR. However, these techniques have limited specificity and selectivity; therefore the development of trustworthy and affordable analytical approaches for EGFR biomarker identification is of utmost importance.…”

“…Due to the associated risk and improved means of ensuring a humane healthcare system, cancer biomarker detection has recently had a noteworthy impact on cancer research. Several biomarkers, EGFR, carcinoembryonic antigen, HER-2, CA-125, CD44, etc., have been categorized as valid biomarkers associated with breast cancer. , In this context, a noteworthy biomarker over cancer tumorigenic cells is the epidermal growth factor receptor (EGFR) for which altered expression and downstream signaling cascades have been reported to be associated with the worsening of the cancerous condition. , Overexpression of EGFR receptors and excessive levels of EGFR antigen have been associated with cancer; therefore increasing research studies have been conducted to detect EGFR in cancer patients’ serum samples. , Enzyme-linked immunosorbent assay, PCR, electron paramagnetic resonance spectrometry, hydrophilic interaction liquid chromatography/mass spectrometry, surface plasmon resonance, , and electrochemical voltammetry methods are some of the analytical techniques currently used to detect EGFR. However, these techniques have limited specificity and selectivity; therefore the development of trustworthy and affordable analytical approaches for EGFR biomarker identification is of utmost importance.…”