A Molecular Clone of HIV-1 Tropic and Cytopathic for Human and Chimpanzee Lymphocytes

Ghosh, Santanu; Fultz, Patricia N.; Keddie, Elise M.; Saag, Michael S.; Sharp, Paul M.; Hahn, Beatrice H.; Shaw, George M.

doi:10.1006/viro.1993.1331

Cited by 70 publications

(76 citation statements)

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“…Despite the fact that most HIV-1 isolates have been shown to produce nonpathogenic infections in chimpanzees, continuing efforts have been made to find or derive an isolate of HIV-1 that is more pathogenic for this species (3,16,33,34). Recent findings suggest that chimpanzees are a natural reservoir for SIVcpz and may have served as the source of HIV-1 introduction into the human population (15).…”

mentioning

confidence: 99%

Rapid CD4⁺T-Cell Loss Induced by Human Immunodeficiency Virus Type 1_NCin Uninfected and Previously Infected Chimpanzees

Novembre¹,

Rosayro²,

Nidtha³

et al. 2001

J Virol

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To investigate the pathogenicity of a virus originating in a chimpanzee with AIDS (C499), two chimpanzees were inoculated with a plasma-derived isolate termed human immunodeficiency virus type 1 NC (HIV-1 NC ). A previously uninfected chimpanzee, C534, experienced rapid peripheral CD4 ؉ T-cell loss to fewer than 26 cells/l by 14 weeks after infection. CD4؉ T-cell depletion was associated with high plasma HIV-1 loads but a low virus burden in the peripheral lymph node. The second chimpanzee, C459, infected 13 years previously with HIV-1 LAV , experienced a more protracted course of peripheral CD4 ؉ T-cell loss after HIV-1 NC inoculation, resulting in fewer than 200 cells/l by 96 weeks postinoculation. The quantities of viral RNA in the plasma and peripheral lymph node from C459 were below the lower limits of detection prior to inoculation with HIV-1 NC but were significantly and persistently increased after superinfection, with HIV-1 NC representing the predominant viral genotype. These results show that viruses derived from C499 are more pathogenic for chimpanzees than any other HIV-1 isolates described to date.

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mentioning

confidence: 99%

Rapid CD4⁺T-Cell Loss Induced by Human Immunodeficiency Virus Type 1_NCin Uninfected and Previously Infected Chimpanzees

Novembre¹,

Rosayro²,

Nidtha³

et al. 2001

J Virol

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“…HIV-gpt HXB2 , pSG3.1, and pNL4-3 were obtained from the AIDS Research and Reference Reagent Program (1,14,29). The construction of HIV-puro BCSG3 , previously referred to as SG3puro BCSG3 , has been described elsewhere (49).…”

Section: Methodsmentioning

confidence: 99%

“…Figure 3 shows a typical HTA gel. A 32 P-labeled single-stranded DNA probe corresponding to HIV-puro BCSG3 nucleotides 7168 to 7987 (according to the BCSG3 RNA sequence) (14) was prepared by asymmetric PCR (Fig. 3, lane P).…”

Section: Generation Of Heterozygous Virionsmentioning

confidence: 99%

High Rate of Recombination throughout the Human Immunodeficiency Virus Type 1 Genome

Jetzt

Klarmann

et al. 2000

J Virol

331

276

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“…Plasmid Construction-pHIV-gpt HXB2 and pSG3.1 were obtained from the AIDS Repository (7,8). In HIV-gpt HXB2 , part of env was replaced with SV-gpt (Escherichia coli xanthine-guanine phosphoribosyltransferase (gpt) gene under control of the SV40 promoter).…”

Section: Methodsmentioning

confidence: 99%

The Nature of Human Immunodeficiency Virus Type 1 Strand Transfers

Jetzt

Ron

et al. 1998

Journal of Biological Chemistry

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The diploid nature of human immunodeficiency virus type 1 (HIV-1) suggests that recombination serves a central function in virus replication and evolution. A system was developed to examine HIV-1 strand transfers, including the obligatory DNA primer strand transfers as well as recombinational crossovers during reverse transcription. Sequence heterogeneity between different strains of HIV-1 was exploited for examining primer transfer events. Both intra-and intermolecular primer transfers were observed at similar frequencies during minus-strand DNA synthesis, whereas primer transfers during plus-strand DNA synthesis were primarily intramolecular. Sequence analysis of long terminal repeats from progeny proviruses also revealed a high rate of homologous recombination during minus-strand synthesis, corresponding to an overall rate of approximately three crossovers per HIV-1 genome per cycle of replication. These results imply that both viral genomic RNAs serve as templates during HIV-1 reverse transcription and that primer strand transfers and recombination may contribute substantially to the rapid genetic variation of HIV-1.

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A Molecular Clone of HIV-1 Tropic and Cytopathic for Human and Chimpanzee Lymphocytes

Cited by 70 publications

References 0 publications

Rapid CD4⁺T-Cell Loss Induced by Human Immunodeficiency Virus Type 1_NCin Uninfected and Previously Infected Chimpanzees

Rapid CD4⁺T-Cell Loss Induced by Human Immunodeficiency Virus Type 1_NCin Uninfected and Previously Infected Chimpanzees

High Rate of Recombination throughout the Human Immunodeficiency Virus Type 1 Genome

The Nature of Human Immunodeficiency Virus Type 1 Strand Transfers

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A Molecular Clone of HIV-1 Tropic and Cytopathic for Human and Chimpanzee Lymphocytes

Cited by 70 publications

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Rapid CD4+T-Cell Loss Induced by Human Immunodeficiency Virus Type 1NCin Uninfected and Previously Infected Chimpanzees

Rapid CD4+T-Cell Loss Induced by Human Immunodeficiency Virus Type 1NCin Uninfected and Previously Infected Chimpanzees

High Rate of Recombination throughout the Human Immunodeficiency Virus Type 1 Genome

The Nature of Human Immunodeficiency Virus Type 1 Strand Transfers

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Product

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Rapid CD4⁺T-Cell Loss Induced by Human Immunodeficiency Virus Type 1_NCin Uninfected and Previously Infected Chimpanzees

Rapid CD4⁺T-Cell Loss Induced by Human Immunodeficiency Virus Type 1_NCin Uninfected and Previously Infected Chimpanzees