A molecular diagnostic for Moniliophthora perniciosa, the causal agent of witches' broom disease of cacao, that differentiates it from its sister taxon Moniliophthora roreri

Silva, Natália Jordana Alves da; Reis, Sara Pereira Menezes; Diorato, Vanessa Santana; Rocha, José Sátiro Almeida; Barbosa, Ceslaine dos Santos; Ciampi‐Guillardi, Maisa; Patrocínio, Nara Geórgia Ribeiro Braz; Niella, Givaldo Rocha; Solis, Karina; Peñaherrera, Sofía; Manco, Maria Julia da Silva; Teixeira, Glauco Antonio; Arévalo‐Gardini, Enrique; Gramacho, Karina Peres

doi:10.1016/j.cropro.2022.106003

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…An alternative hypothesis suggests it originated in the upper Amazon and was introduced to Colombia and Ecuador (Díaz-Valderrama et al 2022). Moniliophthora roreri continues to spread to new areas, including Jamaica ( Johnson et al 2017 ) and western Brazil ( da Silva et al 2022 ). The timing of M. roreri 's transition from sexual to asexual reproduction remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Clonal reproduction of Moniliophthora roreri and the emergence of unique lineages with distinct genomes during range expansion

Minio,

Figueroa-Balderas,

Cohen

et al. 2023

G3: Genes, Genomes, Genetics

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The basidiomycete Moniliophthora roreri causes frosty pod rot of cacao (Theobroma cacao) in the Western hemisphere. M. roreri is considered asexual and haploid throughout its hemibiotrophic lifecycle. To understand the processes driving genome modification, using long-read sequencing technology we sequenced and assembled five high quality M. roreri genomes out of a collection of ninety-nine isolates collected throughout the pathogen's range. We obtained chromosome-scale assemblies composed of eleven scaffolds. We used short-read technology to sequence the genomes of twenty-two similarly chosen isolates. Alignments among the five reference assemblies revealed inversions, translocations, and duplications between and within scaffolds. Isolates at the front of the pathogens’ expanding range tend to share lineage-specific structural variants, as confirmed by short-read sequencing. We identified, for the first time, three new mating type A locus alleles (five in total) and one new potential mating type B locus allele (three in total). Currently only two mating type combinations, A1B1 and A2B2, are known to exist outside of Colombia. A systematic survey of the M. roreri transcriptome across twenty-two isolates identified an expanded candidate effector pool and provided evidence that effector candidate genes unique to the Moniliophthoras are preferentially expressed during the biotrophic phase of disease. Notably, M. roreri isolates in Costa Rica carry a chromosome segment duplication that has doubled the associated gene complement and includes secreted proteins and candidate effectors. Clonal reproduction of the haploid M. roreri genome has allowed lineages with unique genome structures and compositions to dominate as it expands its range, displaying a significant founder effect.

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Section: Discussionmentioning

confidence: 99%

Clonal reproduction of Moniliophthora roreri and the emergence of unique lineages with distinct genomes during range expansion

Minio,

Figueroa-Balderas,

Cohen

et al. 2023

G3: Genes, Genomes, Genetics

View full text Add to dashboard Cite

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“…El ciclo de vida de este patógeno inicia cuando una basidiospora -que es el propágulo infeccioso-alcanza los tejidos en crecimiento o meristemáticos. Allí las hifas monocarióticas se desarrollan y penetran hacia la parte intercelular por estomas o heridas, dando paso a la fase biotrófica que puede cursar con diversa sintomatología dependiendo del órgano infectado, genotipo y condiciones ambientales (Barsottini et al, 2020;Pereira et al, 2022). Esta fase puede llegar a durar de 30 a 60 días antes de dar paso a la fase necrotrófica, donde el hongo adquiere unas características específicas como lo es la producción de micelio dicariótico, el cual es saprófito e infecta el tejido a nivel intracelular.…”

Section: Escoba De Brujaunclassified

BioNanotecnología aplicada al cultivo de Theobroma cacao L

González,

Villamizar

2023

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La Ciencia y la Tecnología son para la vida de los investigadores cien- tíficos, no solo una pasión sino uno de los motores del desarrollo de la sociedad. Por ello, encontrarse con un trabajo científico riguroso que pueda ser aplicado directamente a una problemática social, económica, ambiental y agrícola, es de particular interés y también motivo de admiración. El libro que tiene en sus manos representa el trabajo de varios años de desarrollo científico de un grupo de investigadores con una fuerte motivación por mejorar el conocimiento y entendimiento del cultivo de cacao, sus enfermedades más relevantes y la exploración de diversas metodologías para el control de dichas enfermedades.

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“…During the colonial era, agricultural productivity was enhanced through the introduction of new techniques like shifting cultivation, monoculture, ploughing, and livestock farming [9]. By the 19th century, Ecuador had garnered recognition as a producer of premium cocoa, a crop valued for its ecological and environmental contributions, particularly its promotion of soil biodiversity [10].…”

Section: Introductionmentioning

confidence: 99%

“…However, the 1930s saw the cocoa industry decimated by diseases such as Moniliophthora perniciosa and Crinipellis perniciosa (witches' broom), which reduced cocoa production by 60% and pushed Ecuador off its pedestal as the leading producer [10]. This downturn paved the way for the rise of banana production between 1948 and 1964 [11].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Scientific Contributions to Agricultural Development and Food Security in Ecuador

Herrera-Franco,

Sánchez-Arizo,

Escandón-Panchana

et al. 2023

IJDNE

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In alignment with the 2030 Sustainable Development Goals (SDGs), Ecuador's National Development Plan accentuates SDG 2's commitment to escalating agricultural productivity for food security. Notably, Ecuador contributes significantly as a producer and exporter of foods such as bananas, cocoa, and frozen vegetables, with bananas encompassing 27% of non-oil exports. The past decade has witnessed an appreciable growth in the country's scientific contributions to the agricultural sector. This study employs bibliometrics and systematic reviews, leveraging mapping techniques via bibliometrix and VOSviewer tools, to analyse 1,300 scientific documents spanning from 1973 to 2022. Within this corpus, distinct research trends materialised across three distinct eras. The period from 1973-2000 was characterised by studies on indigenous plants, ivory vegetable production, and the nutritional value of quinoa. The subsequent decade (2001)(2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010) witnessed an expansion into the realms of pollination, properties of Ishpingo oil, genetics, and biomass. The latest period (2011-2022) marked a shift in focus towards cocoa fermentation, genetics, soil analysis, strawberry antioxidants, and eucalyptus properties. Considering Ecuador's growing reliance on global trade integration, the need to address postharvest diseases using biocontrol agents, and the advancement of irrigation automation for water efficiency have emerged as critical areas. The assessment of the quality of scientific contributions in agriculture underscores a direct correlation between food production, the extent of the country's agricultural coverage, and the proportion of employment within the agricultural sector.

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A molecular diagnostic for Moniliophthora perniciosa, the causal agent of witches' broom disease of cacao, that differentiates it from its sister taxon Moniliophthora roreri

Cited by 6 publications

References 23 publications

Clonal reproduction of Moniliophthora roreri and the emergence of unique lineages with distinct genomes during range expansion

Clonal reproduction of Moniliophthora roreri and the emergence of unique lineages with distinct genomes during range expansion

BioNanotecnología aplicada al cultivo de Theobroma cacao L

Analysis of Scientific Contributions to Agricultural Development and Food Security in Ecuador

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