A Molecular Epidemiological Investigation of Carriage of the Adeno-Associated Virus in Murine Rodents and House Shrews in China

Xiong, Yiquan; Zhang, Minyi; Zhou, Junhua; Li, Yongzhi; You, Fang-Fei; Wen, Yiping; He, Wenqiao; Chen, Qing

doi:10.1159/000493799

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…15 AAV infection and NAb are also found in animals such as rodents, cats, pigs, dogs, horses, and chimpanzees. 28,[32][33][34] Although serotypes and NAb titers vary across species, cross-reactivity with many serotypes is common, such as that observed in humans. This finding might be explained by repeated exposure to the living environment and reactivation of latent AAVs integrated in chromosome following infection with the helper virus, including adenovirus.…”

Section: Discussionmentioning

confidence: 99%

“…We have recently examined the seroprevalence of NAbs against nine AAV serotypes and found most NAbs reacted with multiple AAV serotypes in the Japanese population 15 . AAV infection and NAb are also found in animals such as rodents, cats, pigs, dogs, horses, and chimpanzees 28,32–34 . Although serotypes and NAb titers vary across species, cross‐reactivity with many serotypes is common, such as that observed in humans.…”

Section: Discussionmentioning

confidence: 99%

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Successful liver transduction by re‐administration of different adeno‐associated virus vector serotypes in mice

Baatartsogt

Kashiwakura

Hiramoto

et al. 2023

The Journal of Gene Medicine

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Background Intravenous administration of adeno‐associated virus (AAV) vectors is a promising gene therapy approach for monogenic diseases. However, re‐administration of the same AAV serotype is impossible because of the induction of anti‐AAV neutralizing antibodies (NAbs). Here, we examined the feasibility of re‐administrating AAV vector serotypes different from the initial AAV vector serotype. Methods Liver‐targeting AAV3B, AAV5, and AAV8 vectors were intravenously injected in C57BL/6 mice, and the emergence of NAbs and the transduction efficacy following re‐administration were evaluated. Results For all serotypes, re‐administration of the same serotype was not possible. Although the highest neutralizing activity of NAb was induced by AAV5, anti‐AAV5 NAbs did not react with other serotypes, resulting in successful re‐administration with the other serotypes. AAV5 re‐administration was also successful in all mice treated with AAV3B and AAV8. Effective secondary administration of AAV3B and AAV8 was observed in most mice initially administrated AAV8 and AAV3B, respectively. However, few mice developed NAbs cross‐reacting with the other serotypes, especially those with close sequence homology. Conclusions In summary, AAV vector administration induced NAbs relatively specific to the administrated serotype. Secondary administration of AAVs targeting liver transduction could be successfully achieved by switching AAV serotypes in mice.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Successful liver transduction by re‐administration of different adeno‐associated virus vector serotypes in mice

Baatartsogt

Kashiwakura

Hiramoto

et al. 2023

The Journal of Gene Medicine

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“…15 AAV infection and NAb are also found in animals such as rodents, cats, pigs, dogs, horses, and chimpanzee. 27,[30][31][32] Although serotypes and NAb titer vary depending on animals' species, cross-reactivity to many serotypes is common, as in the case with humans. A possible interpretation of this might be related to repeated exposure to the living environment and latent AAVs, integrated into the chromosome and re-activated with the infection of helper virus, including adenovirus.…”

Section: Discussionmentioning

confidence: 99%

Successful Liver transduction by Re-administration of Different Adeno-Associated Virus Vector Serotypes in Mice

Baatartsogt

Kashiwakura

Hiramoto

et al. 2022

Preprint

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Intravenous administration of adeno-associated virus (AAV) vector is a promising gene therapy approach for monogenic diseases. However, re-administration of the same AAV serotype is impossible due to the induction of anti-AAV neutralizing antibodies (NAbs). Here we examined the feasibility of re-administration of AAV vectors to change the serotypes. We administered AAV3B, AAV5, or AAV8 vectors targeting the liver of C57BL/6 mice intravenously, and then assessed the emergence of NAbs and the transduction efficacy with a second administration. For all serotypes, we confirmed that re-administration with the same serotype was not possible. Although the highest neutralizing activity of NAb was induced by AAV5; however, the NAbs elicited by AAV5 did not react with any other serotypes, resulting in success in re-administration with the other serotypes. The re-administration of AAV5 was also successful in all mice treated with AAV3B and AAV8. The effective secondary administration of AAV3B and AAV8 was observed in most mice treated with AAV8 and AAV3B, respectively. However, few mice developed NAbs cross-reactive with the other serotypes, especially the serotypes with close sequence homology. In summary, AAV vector administration induced NAbs relatively specific to the serotype administrated. Secondary administration of AAVs targeting liver transduction could be successfully achieved by switching AAV serotypes in mice.

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“…Adeno-associated virus (AAV), a member of the Dependoparvovirus, was discovered in Asian house shrews in 2020 in China. The Infection status in humans and other animals is unknown [46].…”

Section: Parvoviruses In Shrewsmentioning

confidence: 99%

Shrews (Soricidae) and Viruses Identified in Shrews

Gong,

Chen,

Tan

et al. 2023

Preprint

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Shrews (Soricidae) are common small wild mammals with a significant overlap in their habitats with humans and domestic animals. Currently, viruses from 24 families have been identified in shrews, including Adenoviridae, Arenaviridae, Arteriviridae, Astroviridae, Bornaviridae, Caliciviridae, Circoviridae, Coronaviridae, Filoviridae, Flaviviridae, Hantaviridae, Hepadnaviridae, Hepeviridae, Nairoviridae, Orthoherpesviridae, Paramyxoviridae, Parvoviridae, Phenuiviridae, Picornaviridae, Polyomaviridae, Poxviridae, Rhabdoviridae, Sedoreoviridae, and Spinareoviridae, which pose potential threats to humans and domestic animals. This review compiled fundamental information about shrews and provided a comprehensive summary of the viruses that have been detected in shrews, with the aim to facilitating a deeper understanding of shrews and the diversity and risks of their viruses for researchers and the general public.

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A Molecular Epidemiological Investigation of Carriage of the Adeno-Associated Virus in Murine Rodents and House Shrews in China

Cited by 4 publications

References 23 publications

Successful liver transduction by re‐administration of different adeno‐associated virus vector serotypes in mice

Successful liver transduction by re‐administration of different adeno‐associated virus vector serotypes in mice

Successful Liver transduction by Re-administration of Different Adeno-Associated Virus Vector Serotypes in Mice

Shrews (Soricidae) and Viruses Identified in Shrews

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