2015
DOI: 10.1017/s0007485315000735
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A molecular framework for the identification of planthopper vectors (Hemiptera: Delphacidae) of central Argentina

Abstract: Planthoppers are important worldwide crop pests as well as vectors of numerous diseases. Different species transmit Mal de Río Cuarto virus, which causes the most economically important corn disease in central Argentina. Epidemiological studies rely on the accurate identification of the species present in the field. Presently, morphological identification of planthoppers requires taxonomic expertise and there are no taxonomic keys for females and nymphs. Nevertheless, no molecular protocols are available for a… Show more

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Cited by 6 publications
(3 citation statements)
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“…Later, the primer pair Ron and Calvin was used for molecular detection of planthoppers from the infraorder Fulgoromorpha and the families Cixiidae and Delphacidae [44]. Amplification of delphacid DNA with Ron and Calvin primers was also demonstrated in the study of Argüello Caro et al [37]. In our experiments, cixiid (P. leporinus, R. quinquecostatus, H. obsoletus) and delphacid (J. pellucida, J. obscurella) DNA was amplified.…”
Section: Discussionsupporting
confidence: 64%
See 1 more Smart Citation
“…Later, the primer pair Ron and Calvin was used for molecular detection of planthoppers from the infraorder Fulgoromorpha and the families Cixiidae and Delphacidae [44]. Amplification of delphacid DNA with Ron and Calvin primers was also demonstrated in the study of Argüello Caro et al [37]. In our experiments, cixiid (P. leporinus, R. quinquecostatus, H. obsoletus) and delphacid (J. pellucida, J. obscurella) DNA was amplified.…”
Section: Discussionsupporting
confidence: 64%
“…A list of primers (Table 1) is provided. A ~1000 bp fragment of the COI gene was amplified with primers Ron and Calvin [37] and used as a control for DNA quality. Another PCR protocol was used for amplification of a ~1000 bp fragment of the COI region from R. quinquecostatus and H. obsoletus with primers UEA3 and UEA8 according to Lunt et al [38].…”
Section: Primer Design and Pcr Conditionsmentioning
confidence: 99%
“…Morphological keys are available for specific identification (Wilson and Claridge, 1991). However, no taxonomic keys for the nymphal stage are available (Caro et al, 2015).…”
Section: Identificationmentioning
confidence: 99%