A molecular marker-based linkage map of diploid bananas (Musa acuminata)

Fauré, Sabine; Noyer, Jean‐Louis; Horry, Jean-Pierre; Bakry, Frédéric; Lanaud, Claire; León, Diego González De

doi:10.1007/bf00215098

Cited by 136 publications

(73 citation statements)

References 34 publications

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“…From those libraries a number of molecular markers have been identified and sequenced Miller et al 2008), but their chromosomal positions have not yet been established. In addition, at least one molecular marker based linkage group map has been constructed (Fauré et al 1993).…”

Section: Introductionmentioning

confidence: 99%

The potential of high-resolution BAC-FISH in banana breeding

et al. 2008

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The genetic complexity in the genus Musa has been subject of study in many breeding programs worldwide. Parthenocarpy, female sterility, polyploidy in different cultivars and limited amount of genetic and genomic information make the production of new banana cultivars difficult and time consuming. In addition, it is known that part of the cultivars and related wild species in the genus contain numerous chromosomal rearrangements. In order to produce new cultivars more effectively breeders must better understand the genetic differences of the potential crossing parents for introgression hybridization, but extensive genetic information is lacking.As an alternative to achieve information on genetic collinearity we make use of modern chromosome map technology known as high-resolution fluorescent in situ hybridization (FISH). This article presents the technical aspects and applications of such a technology in Musa species. The technique deals with BAC clone positioning on pachytene chromosomes of Calcutta 4 (Musa acuminata ssp. burmanicoides, A genome group, section Eumusa) and M. velutina (section Rodochlamys). Pollen mother cells digestion with pectolytic enzymes and maceration with acetic acid were optimized for making cell spread preparations appropriate for FISH. As an example of this Euphytica (2009) 166:431-443 DOI 10.1007 approach we chose BAC clones that contain markers to known resistance genes and hybridize them for establishing their relative positions on the two species. Technical challenges for adapting existing protocols to the banana cells are presented. We also discuss how this technique can be instrumental for validating collinearity between potential crossing parents and how the method can be helpful in future mapping initiatives, and how this method allows identification of chromosomal rearrangements between related Musa species and cultivars.

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Section: Introductionmentioning

confidence: 99%

The potential of high-resolution BAC-FISH in banana breeding

et al. 2008

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“…the average loci per group was 12.8. Among the 19 lGs, DWY3 held the most loci (28) and DWU14 and DWY16 the least (7). the longest lG DWY1 covered 146.2 cM, whereas the shortest lG DWY16 covered 29.7cM.…”

Section: Resultsmentioning

confidence: 99%

QTLS Analysis of Several Traits in Longan

Guo

Zhao

Liu

2011

Biotechnology & Biotechnological Equipment

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“…The construction of genetic maps in fruit started 1994 [4]. With the rapid development of molecular biology techniques, great progress has been made in recent years, resulting in genetic maps being constructed for numerous fruit trees, including peach [20,21], pear [22,23], citrus [24], and others [25][26][27]. These days, genetic maps for F1 hybrids of apple are being constructed using various cultivated varieties for both parents, as the closer genetic relationship could highlight more discrete differences.…”

Section: Discussionmentioning

confidence: 99%

Construction of a Genetic Linkage Map and QTL Analysis of Fruit-related Traits in an F1 Red Fuji x Hongrou Apple Hybrid

et al. 2016

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A genetic linkage map of the apple, composed of 175 SSR and 105 SRAP markers, has been constructed using 110 F1 individuals obtained from a cross between the ‘Red Fuji’ Malus domestica and ‘Hongrou’ Malus sieversii cultivars, which have relatively high levels of DNA marker polymorphism and differ remarkably in fruit-related traits. The linkage map comprised 17 linkage groups, covering 1299.67 cM with an average marker distance of 4.6 cM between adjacent markers, or approximately 91% of Malus genome. Linkage groups were well populated and, although marker density ranged from 2.1 to 9.5 cM, just 10 gaps of more than 15 cM were observed. Moreover, just 12.5% of markers displayed segregation distortion. The present genetic linkage map was used to identify quantitative trait loci (QTLs) affecting fruit-related traits. 23 QTLs for ten fruit traits were detected by multiple interval mapping: 3 QTLs for Vc content, One QTL for single fruit weight, 2 QTLs for peel-phenols content, 2 QTLs for flesh-hardness, 2 QTLs for diameter, 6 QTLs for acid content, 1 QTL for sugar content, 2 QTLs for soluble solids content, 2 QTLs for flesh-phenols and 2 QTLs for brittleness. These QTLs were located on linkage groups C1, C2, C3, C5, C6, C7, C9, C10, C14 and C17, respectively. The phenotypic variations exhibited by each QTL ranged from 2% to 72%, and their LOD values varied from 2.03 to 8.93, of which five QTLs were major effect genes (R2 ≥ 10%). The tight linkage markers (*me2em7-460f, *MS01a03-180m, *me1em6-307m, *CH05c06-102f, *me1em8-423f) would be helpful to elucidate the molecular mechanisms of apple domestication and breeding in the future.

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A molecular marker-based linkage map of diploid bananas (Musa acuminata)

Cited by 136 publications

References 34 publications

The potential of high-resolution BAC-FISH in banana breeding

The potential of high-resolution BAC-FISH in banana breeding

QTLS Analysis of Several Traits in Longan

Construction of a Genetic Linkage Map and QTL Analysis of Fruit-related Traits in an F1 Red Fuji x Hongrou Apple Hybrid

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