A molecular phylogeny of Anopheles annulipes (Diptera: Culicidae) sensu lato: The most species-rich anopheline complex

Foley, Desmond H.; Wilkerson, Richard C.; Cooper, Robert D.; Volovsek, Miguel; Bryan, Joan H.

doi:10.1016/j.ympev.2006.10.008

Cited by 48 publications

(34 citation statements)

References 30 publications

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“…A 3% value is used as the speciation threshold, 73 however, a lower threshold was set to separate all of the Annulipes Complex species. 74 It is argued that the 3% assessment would minimizing false positives, but it may also generate false negatives, and that intraspecific variation is well constrained in the 2-3% range, 75 values that are comparable to the ones found for the NW and SE lineages.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Anopheles triannulatus s.l. (Diptera: Culicidae) from Northwestern and Southeastern Colombia

Rosero-García¹,

Jaramillo²,

Gutiérrez³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Anopheles triannulatus s.l. is a species complex, however in Colombia its taxonomic status is unclear. This study was conducted to understand the level of genetic differentiation or population structure of specimens of An. triannulatus s.l. from northwestern and southeastern Colombia. Cytochrome oxidase subunit I (COI) and internal transcribed spacer (ITS2) sequence analyses suggested high genetic differentiation between the NW and SE populations. A TCS network and Bayesian inference analysis based on 814 bp of COI showed two main groups: group I included samples from the NW and group II samples from the SE. Two main ITS2-polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) patterns were found. Pattern I is present in both the NW and SE, and pattern II is found in the SE specimens. To further elucidate the taxonomic status of An. triannulatus s.l. in Colombia and how these COI lineages are related to the Triannulatus Complex species, the evaluation of immature stages, male genitalia, and additional mitochondrial and nuclear markers will be needed.

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Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Anopheles triannulatus s.l. (Diptera: Culicidae) from Northwestern and Southeastern Colombia

Rosero-García¹,

Jaramillo²,

Gutiérrez³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…These loci are unlinked and evolve separately, thereby giving independent estimates of genetic lineages and relationships. Numerous mosquito barcoding studies have used both mitochondrial and nuclear markers to distinguish species (Foley et al 2007; Paredes-Esquivel et al 2009; Puslednik et al 2012; Bourke et al 2013). ITS2 is often used in mosquito studies as its high evolutionary rate makes it useful for investigating closely related species, such as those in the Anopheles genus (Wilkerson et al 2004; Marrelli et al 2006; Walton et al 2007; Sum et al 2014).…”

mentioning

confidence: 99%

Using Next-Generation Sequencing for DNA Barcoding: Capturing Allelic Variation in ITS2

Batovska

Cogan

Lynch

et al. 2017

G3 Genes|Genomes|Genetics

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Internal Transcribed Spacer 2 (ITS2) is a popular DNA barcoding marker; however, in some animal species it is hypervariable and therefore difficult to sequence with traditional methods. With next-generation sequencing (NGS) it is possible to sequence all gene variants despite the presence of single nucleotide polymorphisms (SNPs), insertions/deletions (indels), homopolymeric regions, and microsatellites. Our aim was to compare the performance of Sanger sequencing and NGS amplicon sequencing in characterizing ITS2 in 26 mosquito species represented by 88 samples. The suitability of ITS2 as a DNA barcoding marker for mosquitoes, and its allelic diversity in individuals and species, was also assessed. Compared to Sanger sequencing, NGS was able to characterize the ITS2 region to a greater extent, with resolution within and between individuals and species that was previously not possible. A total of 382 unique sequences (alleles) were generated from the 88 mosquito specimens, demonstrating the diversity present that has been overlooked by traditional sequencing methods. Multiple indels and microsatellites were present in the ITS2 alleles, which were often specific to species or genera, causing variation in sequence length. As a barcoding marker, ITS2 was able to separate all of the species, apart from members of the Culex pipiens complex, providing the same resolution as the commonly used Cytochrome Oxidase I (COI). The ability to cost-effectively sequence hypervariable markers makes NGS an invaluable tool with many applications in the DNA barcoding field, and provides insights into the limitations of previous studies and techniques.

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“…All these species discovered with molecular methods can help to define species boundaries and fos- ter further, targeted morphological studies, which can lead to the discovery of new morphological characters (Moritz & Cicero, 2004). Currently, it is not clear what level of divergence designates a significant difference within and between lineages and some authors employ a 2-3% threshold (Hebert et al, 2003;Meyer & Paulay, 2005;Meier et al, 2008;Foley et al, 2007). Hajibabaei et al (2006) suggested that 4.5-6.0% divergence in COI mtDNA sequences is enough to discriminate between congeneric species in Lepidoptera.…”

Section: Discussionmentioning

confidence: 99%

Identifying cryptic speciation across groundwater populations: first COI sequences of Bathynellidae (Crustacea, Syncarida)

Garcı́a-Parı́s¹,

Dorda²,

Madeira³

2011

GRAELLSIA

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The biodiversity of groundwater fauna remains poorly known and understood. Groundwater biodiversity studies are strongly affected by habitat inaccessibility and taxonomic crisis. The objective of this work was to investigate levels of genetic divergence across populations of Bathynellacea, a small crustacean group that lives exclusively in groundwater, in order to evaluate the extent of cryptic speciation in morphologically constrained clades. Partial sequences of cytochrome oxidase I (COI) have been obtained, for the first time in Bathynellidae. Specimens analyzed of the genus Vejdovskybathynella were obtained from six populations morphologically assignable to a single species; all of them are located in different areas of one of the largest karst systems (110 km of galleries topographied) known in Spain. The analyses of molecular data demonstrate the presence of three highly divergent genetic units, possibly corresponding to undescribed new species. The results of this study provide the first molecular data that complement morphological knowledge in order to address phylogenetic studies to try to resolve the relations between genera and species of the Bathynellidae family. We conclude that the evolutionary scenario of this special group of subterranean crustaceans cannot be revealed only by using morphological information due to the presence of very old lineages of cryptic species, as has been brought to light with the molecular data obtained here.

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A molecular phylogeny of Anopheles annulipes (Diptera: Culicidae) sensu lato: The most species-rich anopheline complex

Cited by 48 publications

References 30 publications

Genetic Diversity of Anopheles triannulatus s.l. (Diptera: Culicidae) from Northwestern and Southeastern Colombia

Genetic Diversity of Anopheles triannulatus s.l. (Diptera: Culicidae) from Northwestern and Southeastern Colombia

Using Next-Generation Sequencing for DNA Barcoding: Capturing Allelic Variation in ITS2

Identifying cryptic speciation across groundwater populations: first COI sequences of Bathynellidae (Crustacea, Syncarida)

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