A Molecular Phylogeny of the Aphidiinae (Hymenoptera: Braconidae)

Belshaw, Robert; Quicke, Donald L. J.

doi:10.1006/mpev.1996.0400

Cited by 219 publications

(178 citation statements)

References 44 publications

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“…Elongation factor-1␣ (EF-1␣) has been widely used to infer phylogenies (e.g., Cho et al, 1995;Belshaw and Quicke, 1997;Friedlander et al, 1998). It is one of the most abundant proteins in eukaryotic cells, in which it catalyzes the binding of charged tRNAs to the ribosome during translation.…”

Section: Utility Of Molecular Markersmentioning

confidence: 99%

Evidence for Three Major Clades within the Snapping Shrimp Genus Alpheus Inferred from Nuclear and Mitochondrial Gene Sequence Data

Williams

Knowlton

Weigt

et al. 2001

Molecular Phylogenetics and Evolution

101

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Section: Utility Of Molecular Markersmentioning

confidence: 99%

Evidence for Three Major Clades within the Snapping Shrimp Genus Alpheus Inferred from Nuclear and Mitochondrial Gene Sequence Data

Williams

Knowlton

Weigt

et al. 2001

Molecular Phylogenetics and Evolution

101

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“…Protocolo 5 -modifi cado a partir de Belshaw & Quicke (1997): Insetos inteiros foram macerados em 700 l de NaCl 5 M. Em seguida adicionou-se 1 l de proteinase K (20 mg ml -1 ) e incubou-se as amostras por 18h a 37ºC. Posteriormente, estas foram centrifugadas por 7 min a 12484 g e o pellet foi lavado com 500 l de acetato de sódio 0,2 M em etanol 76%.…”

Section: Methodsunclassified

Diversidade genética de Tibraca limbativentris Stål (Hemiptera: Pentatomidae) de Santa Catarina e do Rio Grande do Sul, usando marcadores RAPD

Rampelotti¹,

Ferreira²,

Tcacenco³

et al. 2008

Neotrop. entomol.

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-The work was carried out to test DNA extraction protocols and to characterize populations of Tibraca limbativentris Stål, an important rice insect-pest. Insects were collected in Joinville, Rio do Oeste and Turvo, in Santa Catarina State, and Agudo, Uruguaiana, Pelotas and Palmares do Sul, in Rio Grande do Sul State, and six literature-referenced protocols, besides a new one, were tested. DNA from ten individuals of each population was extracted using the best protocol and RAPD reactions were carried out with ten initiators. The new protocol showed the best results and was used in the PCR reactions, that generated 151 polymorphic bands, allowing to access genetic differences among all the populations; no individuals from one population were clustered with individuals from another. The largest intrapopulacional similarity was found in Uruguaiana (22%), and the smallest in Palmares do Sul (50%), which was also the most divergent population in relation to the others. The Gst was 0.5215, and the Nm was 0.4588; these values refl ect the low similarity between the populations. The smallest genic fl ow was obtained when Palmares do Sul and Pelotas were included in the comparisons, in accordance with the largest divergence of these two populations in relation to the others. There was no signifi cant relation between geographic distance and genetic similarity, which can refl ect unknown model of dispersion of T. limbativentris. New studies exploring the species dispersion strategies may help to understand the insect distribution and to unveil the main factors linked to the genetic variability within and between populations.KEY WORDS: Insecta, rice stem bug, irrigated rice, molecular marker RESUMO -O trabalho objetivou testar protocolos de extração de DNA e caracterizar populações de Tibraca limbativentris, Stål, importante inseto-praga do arroz. Os insetos foram coletados em Joinville, Rio do Oeste e Turvo, em Santa Catarina, e Agudo, Uruguaiana, Pelotas e Palmares do Sul, no Rio Grande do Sul. Testaram-se seis protocolos de extração de DNA citados na literatura, e um novo protocolo adequado à espécie em questão. DNA de dez indivíduos de cada população foi extraído usando o melhor protocolo e reações de RAPD foram realizadas com dez iniciadores. O novo protocolo mostrou os melhores resultados e foi utilizado nas reações de PCR, que geraram 151 bandas polimórfi cas, permitindo acessar diferenças genéticas entre todas as populações; não ocorreram indivíduos de uma população agrupados com os de outra. A maior similaridade intrapopulacional foi encontrada em Uruguaiana (22%), e a menor em Palmares do Sul (50%), também a população mais divergente das demais. O valor Gst foi 0,5215, e de Nm 0,4588; esses valores refl etem a pouca similaridade entre as populações. O menor Nm foi apresentado quando Palmares do Sul e Pelotas foram incluídos nas comparações, em consonância com a maior divergência apresentada por essas populações em relação às outras. Não se observou relação entre a distância geográfi ca e a similaridade ...

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“…The forward primer (28S D2 f) from Belshaw and Quicke (1997) and the reverse primer (28S D2 r) from Campbell et al (1993) were used to obtain an ampliÞed fragment of 427Ð510 bp. The polymerase chain reaction (PCR) conditions were 30 cycles of 98ЊC denaturation (15 s), 49ЊC annealing (30 s), and 72ЊC elongation (40 s) with an initial denaturation of 3 min at 93ЊC and a Þnal elongation at 72ЊC for 3 min.…”

Section: Methodsmentioning

confidence: 99%

“…The wobble primers designed by Belshaw and Quicke (1997) were used to amplify a 231Ð 424-bp fragment. The PCR conditions were 35 cycles of 92ЊC denaturation (1 min), 53ЊC annealing (1 min), and 72ЊC extension (1 min), with an initial denaturation of 1 min 30 s at 94ЊC and a Þnal extension of 3 min at 72ЊC.…”

Section: Methodsmentioning

confidence: 99%

Phylogeography ofChelonus insularis(Hymenoptera: Braconidae) andCampoletis sonorensis(Hymenoptera: Ichneumonidae), Two Primary Neotropical Parasitoids of the Fall Armyworm (Lepidoptera: Noctuidae)

Jourdie

Virla

Murillo

et al. 2010

Annals of the Entomological Society of America

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In a previous study, we observed no spatial genetic structure in Mexican populations of the parasitoids Chelonus insularis Cresson (Hymenoptera: Braconidae) and Campoletis sonorensis Cameron (Hymenoptera: Ichneumonidae) by using microsatellite markers. In the current study, we investigated whether for these important parasitoids of the fall armyworm (Lepidoptera: Noctuidae) there is any genetic structure at a larger scale. Insects of both species were collected across the American continent and their phylogeography was investigated using both nuclear and mitochondrial markers. Our results suggest an ancient northÐsouth migration of C. insularis, whereas no clear pattern could be determined for C. sonorensis. Nonetheless, the resulting topology indicated the existence of a cryptic taxon within this later species: a few Canadian specimens determined as C. sonorensis branch outside a clade composed of the Argentinean Chelonus grioti Blanchard, the Brazilian Chelonus flavicincta Ashmead, and the rest of the C. sonorensis individuals. The individuals revealing the cryptic taxon were collected from Trichoplusia ni (Hü bner) (Lepidoptera: Noctuidae) on tomato (Lycopersicon spp.) and may represent a biotype that has adapted to the early season phenology of its host. Overall, the loosely deÞned spatial genetic structure previously shown at a local Þne scale also was found at the larger scale, for both species. Dispersal of these insects may be partly driven by wind as suggested by genetic similarities between individuals coming from very distant locations.

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A Molecular Phylogeny of the Aphidiinae (Hymenoptera: Braconidae)

Cited by 219 publications

References 44 publications

Evidence for Three Major Clades within the Snapping Shrimp Genus Alpheus Inferred from Nuclear and Mitochondrial Gene Sequence Data

Evidence for Three Major Clades within the Snapping Shrimp Genus Alpheus Inferred from Nuclear and Mitochondrial Gene Sequence Data

Diversidade genética de Tibraca limbativentris Stål (Hemiptera: Pentatomidae) de Santa Catarina e do Rio Grande do Sul, usando marcadores RAPD

Phylogeography ofChelonus insularis(Hymenoptera: Braconidae) andCampoletis sonorensis(Hymenoptera: Ichneumonidae), Two Primary Neotropical Parasitoids of the Fall Armyworm (Lepidoptera: Noctuidae)

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