A molecular scheme for the reaction between γ-aminobutyric acid and the most abundant chloride channel on crayfish deep extensor abdominal muscle

Adelsberger, Helmuth; Beckerath, Nicolas von; Parzefall, Franz; Dudél, J.

doi:10.1007/bf02253830

Cited by 10 publications

(1 citation statement)

References 17 publications

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“…The present investigation was performed with a chloride channel expressed after axotomy on crayfish deep extensor abdominal muscle. In comparison to the chloride channel on non-axotomized muscles (Von Beckerath et al, 1995;Adelsberger et al, 1996b), the postulated molecular reaction scheme [Scheme (1)] of this channel is less complicated and the channel density is about hundredfold higher (Adelsberger et al, 1996a), increasing the chance to obtain patches containing a large number of channels which is useful when blocks are investigated.…”

Section: Introductionmentioning

confidence: 99%

Block by picrotoxin of a GABAergic chloride channel expressed on crayfish muscle after axotomy

Adelsberger¹,

Brunswieck²,

Dudél³

1998

Eur J of Neuroscience

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Outside-out patches containing a gamma-aminobutyric acid (GABA)-activated chloride channel expressed after axotomy on crayfish deep extensor abdominal muscle were excised. GABA and the blocker picrotoxin (PTX) were applied to the patches using a liquid filament switch to study the effects of picrotoxin on the GABA-elicited currents. Coapplication of GABA and PTX resulted in a reduction of the current amplitude compared with that elicited by the same GABA concentration alone. This reduction of the amplitude was dependent on both the GABA and PTX concentrations. The rise time of the current decreased after coapplication of GABA and PTX. Evaluation of the single channel currents and off-currents in the presence of GABA and PTX showed a dramatic shortening of the burst duration of the channel. The open time distributions were not altered, whereas in the closed time distributions a new closed time was apparent in presence of PTX. Preincubation with PTX prior to the GABA pulse resulted in an increase of the rise time. This effect was dependent on the PTX concentration only. Possible mechanisms are discussed to explain the effects of PTX and are implemented into the existing molecular reaction scheme of the channel.

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Section: Introductionmentioning

confidence: 99%

Block by picrotoxin of a GABAergic chloride channel expressed on crayfish muscle after axotomy

Adelsberger¹,

Brunswieck²,

Dudél³

1998

Eur J of Neuroscience

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Crustacean Neuromuscular Glutamatergic and GABAergic Channels with Some Comparison to Drosophila Channels

Dudél¹,

Adelsberger²,

Heckmann³

2002

The Crustacean Nervous System

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Neuromuscular glutamatergic and GABAergic channels

Dudél¹,

Adelsberger²,

Heckmann³

1997

Invertebrate Neuroscience

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Our laboratory has worked extensively on glutamatergic and GABA-ergic channels, predominantly in crayfish, but also in locust, Drosophila and recently Ascaris. Channel currents were recorded in the different modes of the patch-clamp technique (Hamill et al., 1981). The opening kinetics of the channels were derived from open and closed time histograms obtained from single channel recordings. From these, channel conductances could also be evaluated. The most relevant data were obtained by very rapidly rising and falling pulses (time of change about 0.1 ms) of agonists applied to outside-out patches containing the respective channels (Franke et al., 1987). From such recordings we constructed dose-response curves for peak and steady-state currents, for the rise times of the currents and for the time constants of desensitization. In double-pulse experiments we measured recovery from desensitization and predesensitization due to low agonist concentrations. For most of the channel types, we succeeded in constructing a reaction scheme which in computer simulations mimicked channel behaviour to a good approximation.

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A molecular scheme for the reaction between γ-aminobutyric acid and the most abundant chloride channel on crayfish deep extensor abdominal muscle

Cited by 10 publications

References 17 publications

Block by picrotoxin of a GABAergic chloride channel expressed on crayfish muscle after axotomy

Block by picrotoxin of a GABAergic chloride channel expressed on crayfish muscle after axotomy

Crustacean Neuromuscular Glutamatergic and GABAergic Channels with Some Comparison to Drosophila Channels

Neuromuscular glutamatergic and GABAergic channels

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