A molecular transport medium for collection, inactivation, transport, and detection of &lt;I&gt;Mycobacterium tuberculosis&lt;/I&gt;

Daum, Luke T.; Choi, Young Wook; Worthy, Sue A.; Rodriguez, J. D.; Chambers, James P.; Fischer, Gerald W.

doi:10.5588/ijtld.13.0021

Cited by 17 publications

(21 citation statements)

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“…The rapid inactivation is in line with findings from Daum et al (2014), which showed that PS-MTM is an effective medium to inactivate a pure culture of M. tuberculosis at ~10 6 CFU/ml at an exposure time of 30 minutes. This is a similar effect as achieved by the Xpert SR buffer where an 8 and 9 log reduction was demonstrated at 15 minutes and 2 hours respectively (Helb, et al, 2010).…”

Section: Discussionsupporting

confidence: 87%

“…Expanding potential use of PS-MTM to TB diagnostics, a recent study of PS-MTM showed inactivation within 30 min of M. tuberculosis at a bacillary load of 10 6 CFU/ml (Daum et al, 2014). Based on the relatively scant information on the use of PS-MTM for TB diagnosis and research, as indicated above, insight into its potential application in routine diagnostics of TB is warranted.…”

Section: Introductionmentioning

confidence: 99%

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Laboratory evaluation of a specimen transport medium for downstream molecular processing of sputum samples to detect Mycobacterium tuberculosis

Omar

Peters

Ismail

et al. 2015

Journal of Microbiological Methods

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Background: Modern molecular-based approaches for the detection of M. tuberculosis in sputum samples promise quicker and more accurate detection of cases. However, processing sputum samples at central diagnostic facilities provides a diagnostic approach, but requires a safe and efficient system that is not affected by transport delays and ambient temperature to be feasible. We evaluated the technical properties of PrimeStore® -Molecular Transport Medium (PS-MTM) for its ability to inactivate mycobacteria, ensuring stability of DNA over time at ambient temperatures and to assess the compatibility of the transport medium with DNA extraction systems. Conclusions: Collecting and transporting sputum from TB suspects in PS-MTM offers safe transport at ambient temperature, DNA stability for extended periods without cooling and specimens directly suitable for molecular testing. This novel approach may support introduction and further scale-up of molecular diagnostics for TB in resource-limited settings.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Laboratory evaluation of a specimen transport medium for downstream molecular processing of sputum samples to detect Mycobacterium tuberculosis

Omar

Peters

Ismail

et al. 2015

Journal of Microbiological Methods

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show abstract

“…We have previously shown that this is a safe way of transporting sputum specimens as PS‐MTM inactivates the bacilli, thereby making it safe from an infection control perspective, and that stability of DNA is achieved over time at ambient temperature . These findings concurred with previous studies evaluating these technical properties . In our laboratory evaluation, we further demonstrated good sensitivity and specificity of IS6110 real‐time PCR detection of M. tuberculosis from clinical samples inoculated into PS‐MTM .…”

Section: Discussionsupporting

confidence: 88%

Field evaluation of a novel preservation medium to transport sputum specimens for molecular detection of Mycobacterium tuberculosis in a rural African setting

Omar

Peters

Ismail

et al. 2016

Tropical Med Int Health

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Abstractobjectives To assess the performance of an innovative method of transporting sputum to centralised facilities for molecular detection of Mycobacterium tuberculosis: using a swab to inoculate sputum in a transport medium, PrimeStore â Molecular Transport Medium (PS-MTM).methods Two sputum specimens were obtained from suspected patients with tuberculosis (TB) at rural healthcare facilities in South Africa. A swab was taken from each specimen and placed into PS-MTM, prior to it being processed by either liquid culture or Xpert MTB/RIF assay (Xpert).results A total of 141 patients (including 47 with laboratory-confirmed TB) were included in this analysis. M. tuberculosis was detected at 29% by culture and 29% by Xpert, whereas 31% tested positive by IS6110 real-time PCR of PS-MTM from the culture and 36% from the Xpert-paired specimen. Concordance between the method under evaluation with culture was 82% (McNemar, P = 0.55) and 84% (McNemar, P = 0.05) for Xpert. Stratified by culture result, the detection rate by IS6110 real-time PCR of PS-MTM was similar to Xpert for patients with positive culture (P = 0.32), but significantly higher if culture was negative (P = 0.008).conclusions These results suggest that swab collection of sputum into PS-MTM for transport is a promising method for diagnosis of TB in rural healthcare settings, thereby potentially improving the options available for molecular diagnosis of TB in countries incapable of applying decentralised high-tech molecular testing.

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“…The sputum samples were placed on ice immediately after collection and within an hour of collection.~0.5 ml of sputum was transferred to Primestore Molecular Transport Media (Longhorn Vaccines and Diagnostics) vial already containing 1.5 ml of a proprietary mixture of components meant to kill and lyse M . tb in the sputum and to retain the integrity of the released nucleic acid [14,15] as per the PrimeStore manufacturer’s instructions. For optimization of RNA isolation techniques from sputum samples in Primestore, M .…”

Section: Methodsmentioning

confidence: 99%

Transcriptome analysis of mycobacteria in sputum samples of pulmonary tuberculosis patients

et al. 2017

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Pulmonary tuberculosis, the disease caused by Mycobacterium tuberculosis, still retains a top rank among the deadliest communicable diseases. Sputum expectorated during the disease continues to be a primary diagnostic specimen and also serves as a reservoir of bacteria. The expression pattern of mycobacteria in sputum will lead to an insight into bacterial adaptation at the most highly transmissible stage of infection and can also help in identifying newer diagnostic as well as drug targets. Thus, in the present study, a whole genome microarray of Mycobacterium tuberculosis was used to elucidate the transcriptional profile of mycobacteria in the sputum samples of smear positive pulmonary tuberculosis patients. Overall, the mycobacteria in sputum appeared to be in a low energy and low replicative state as compared to in vitro grown log phase M. tb with downregulation of genes involved in ATP synthesis, aerobic respiration and translational machinery. Simultaneously, downregulation was also seen in the genes involved in secretion machinery of mycobacteria along with the downregulation of genes involved in the synthesis of phthiocerol dimycocerosate and phenol glycolipids. In contrast, the majority of the genes which showed an upregulation in sputum mycobacteria were of unknown function. Further identification of these genes may provide new insights into the mycobacterial behavior during this phase of infection and may help in deciphering candidates for development of better diagnostic and drug candidates.

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A molecular transport medium for collection, inactivation, transport, and detection of <I>Mycobacterium tuberculosis</I>

Cited by 17 publications

References 0 publications

Laboratory evaluation of a specimen transport medium for downstream molecular processing of sputum samples to detect Mycobacterium tuberculosis

Laboratory evaluation of a specimen transport medium for downstream molecular processing of sputum samples to detect Mycobacterium tuberculosis

Field evaluation of a novel preservation medium to transport sputum specimens for molecular detection of Mycobacterium tuberculosis in a rural African setting

Transcriptome analysis of mycobacteria in sputum samples of pulmonary tuberculosis patients

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