A Moloney Murine Leukemia Virus Driven by the Jaagsiekte Sheep Retrovirus Enhancers Shows Enhanced Specificity for Infectivity in Lung Epithelial Cells

McGee-Estrada, Kathleen; Palmarini, Massimo; Hallwirth, Claus V.; Fan, Hung

doi:10.1007/s11262-005-3239-y

Cited by 12 publications

(6 citation statements)

References 23 publications

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“…The tissue specificity of the JSRV LTR was further demonstrated when JSRV U3 enhancer sequences were inserted into a Moloney murine leukemia virus (M-MuLV) LTR lacking its own enhancers [134]. M-MuLV driven by this chimeric LTR showed enhanced infectivity in lung epithelial cells that otherwise were not permissive for M-MuLV infection [134].…”

Section: Transcriptional Specificity Of Jsrvmentioning

confidence: 99%

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Jaagsiekte Sheep Retrovirus Biology and Oncogenesis

Hofacre

Fan

2010

Viruses

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Jaagsiekte sheep retrovirus (JSRV) is the causative agent of a lung cancer in sheep known as ovine pulmonary adenocarcinoma (OPA). The disease has been identified around the world in several breeds of sheep and goats, and JSRV infection typically has a serious impact on affected flocks. In addition, studies on OPA are an excellent model for human lung carcinogenesis. A unique feature of JSRV is that its envelope (Env) protein functions as an oncogene. The JSRV Env-induced transformation or oncogenesis has been studied in a variety of cell systems and in animal models. Moreover, JSRV studies have provided insights into retroviral genomic RNA export/expression mechanisms. JSRV encodes a trans-acting factor (Rej) within the env gene necessary for the synthesis of Gag protein from unspliced viral RNA. This review summarizes research pertaining to JSRV-induced pathogenesis, Env transformation, and other aspects of JSRV biology.

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Section: Transcriptional Specificity Of Jsrvmentioning

confidence: 99%

“…M-MuLV driven by this chimeric LTR showed enhanced infectivity in lung epithelial cells that otherwise were not permissive for M-MuLV infection [134]. Two additional motifs in the JSRV LTR that bind C/EBP and NF-1 isoforms were later shown to be important for LTR activity as well [135].…”

Section: Transcriptional Specificity Of Jsrvmentioning

confidence: 99%

Jaagsiekte Sheep Retrovirus Biology and Oncogenesis

Hofacre

Fan

2010

Viruses

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“…The substituted enhancer within the modified U3 region is copied to the 5′ LTR during RT, which thereby disrupts the original 5′ LTR, and the inserted enhancer will instead drive gene expression ( Fig. S3 B2 and B3) (35)(36)(37)(38)(39)(40).…”

Section: Resultsmentioning

confidence: 99%

“…Here we show that PRVs infect sea urchin embryos, integrating genomically with a copy number of one per genome. We successfully used self-inactivation (SIN) strategies from gene therapy approaches (35)(36)(37)(38)(39)(40) to simultaneously insert a global, maternal-plus-zygotic sea urchin enhancer module and disable the endogenous viral enhancer in the viral backbone. The results demonstrate that SIN PRVs integrate genomically and drive global and persistent gene expression.…”

mentioning

confidence: 99%

Pantropic retroviruses as a transduction tool for sea urchin embryos

Core¹,

Reyna²,

Conaway³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Sea urchins are an important model for experiments at the intersection of development and systems biology, and technical innovations that enhance the utility of this model are of great value. This study explores pantropic retroviruses as a transduction tool for sea urchin embryos, and demonstrates that pantropic retroviruses infect sea urchin embryos with high efficiency and genomically integrate at a copy number of one per cell. We successfully used a self-inactivation strategy to both insert a sea urchin-specific enhancer and disrupt the endogenous viral enhancer. The resulting self-inactivating viruses drive global and persistent gene expression, consistent with genomic integration during the first cell cycle. Together, these data provide substantial proof of principle for transduction technology in sea urchin embryos.

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“…We previously studied the transcriptional specificity of the JSRV LTR in transient transfection assays (Palmarini et al, 2000), (McGee-Estrada, Palmarini, and Fan, 2002;McGee-Estrada et al, 2005). A reporter plasmid consisting of the JSRV LTR driving the firefly luciferase gene was tested in a variety of murine cell lines derived from different cell types.…”

Section: Introductionmentioning

confidence: 99%

Specific in vivo expression in type II pneumocytes of the jaagsiekte sheep retrovirus long terminal repeat in transgenic mice

Dakessian

Fan

2008

Virology

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Jaagsiekte sheep retrovirus (JSRV) is the causative agent of ovine pulmonary adenocarcinoma, a transmissible lung cancer in sheep. Previous experiments in differentiated murine tissue culture cell lines suggested that the disease specificity of JSRV for secretory lung epithelial cells (type II pneumocytes an Clara cells) reflects transcriptional specificity of the viral long terminal repeat (LTR) for these cells. To test this in vivo, transgenic mice carrying the bacterial beta-galactosidase (beta-Gal) gene driven by the JSRV LTR were generated. Two transgenic lines showed beta-Gal expression in the lungs but not other tissues of F1 animals, although transgene silencing in subsequent generations was a major problem. The cells expressing the transgene were identified by two- and three-color immunofluorescence for marker proteins of type II pneumocytes (surfactant protein C [SPC]) and Clara cells (CC10) as well as for a T7 gene 10 epitope present in the beta-Gal reporter. F1 animals from both lines showed transgene expression in type II pneumocytes, but somewhat surprisingly not in Clara cells. Expression was not detected in bronchiolo-alveolar stem cells (BASCs) either. These results indicate that the JSRV LTR is specifically active in type II pneumocytes in the mouse lung, which is consistent with the fact that JSRV-induced OPA tumors in sheep largely have phenotypic markers of type II pneumocytes.

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A Moloney Murine Leukemia Virus Driven by the Jaagsiekte Sheep Retrovirus Enhancers Shows Enhanced Specificity for Infectivity in Lung Epithelial Cells

Cited by 12 publications

References 23 publications

Jaagsiekte Sheep Retrovirus Biology and Oncogenesis

Jaagsiekte Sheep Retrovirus Biology and Oncogenesis

Pantropic retroviruses as a transduction tool for sea urchin embryos

Specific in vivo expression in type II pneumocytes of the jaagsiekte sheep retrovirus long terminal repeat in transgenic mice

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