A monoclonal antibody and an enzyme immunoassay for human Ala-IL-877

Nashkevich, Natalia; Akalovich, Svetlana; Louneva, Natalia; Heavner, George A.; Voitenok, Nikolai N.

doi:10.1016/s0022-1759(02)00279-x

Cited by 5 publications

(3 citation statements)

References 43 publications

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“…Concentration of IL-8 77 was measured as previously described [34] , except samples were detected by the detection-antibody from the commercial IL-8 ELISA described above. Lower limit of sensitivity of the assay was 3.125 pg/ml (Figure S2 in File S1 ).…”

Section: Methodsmentioning

confidence: 99%

Role of Serine Proteases in the Regulation of Interleukin-877 during the Development of Bronchopulmonary Dysplasia in Preterm Ventilated Infants

et al. 2014

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RationaleThe chemokine interleukin-8 is implicated in the development of bronchopulmonary dysplasia in preterm infants. The 77-amino acid isoform of interleukin-8 (interleukin-877) is a less potent chemoattractant than other shorter isoforms. Although interleukin-877 is abundant in the preterm circulation, its regulation in the preterm lung is unknown.ObjectivesTo study expression and processing of pulmonary interleukin-877 in preterm infants who did and did not develop bronchopulmonary dysplasia.MethodsTotal interleukin-8 and interleukin-877 were measured in bronchoalveolar lavage fluid from preterm infants by immunoassay. Neutrophil serine proteases were used to assess processing. Neutrophil chemotaxis assays and degranulation of neutrophil matrix metalloproteinase-9 were used to assess interleukin-8 function.Main ResultsPeak total interleukin-8 and interleukin-877 concentrations were increased in infants who developed bronchopulmonary dysplasia compared to those who did not. Shorter forms of interleukin-8 predominated in the preterm lung (96.3% No-bronchopulmonary dysplasia vs 97.1% bronchopulmonary dysplasia, p>0.05). Preterm bronchoalveolar lavage fluid significantly converted exogenously added interleukin-877 to shorter isoforms (p<0.001). Conversion was greater in bronchopulmonary dysplasia infants (p<0.05). This conversion was inhibited by α-1 antitrypsin and antithrombin III (p<0.01). Purified neutrophil serine proteases efficiently converted interleukin-877 to shorter isoforms in a time- and dose-dependent fashion; shorter interleukin-8 isoforms were primarily responsible for neutrophil chemotaxis (p<0.001). Conversion by proteinase-3 resulted in significantly increased interleukin-8 activity in vitro (p<0.01).ConclusionsShorter, potent, isoforms interleukin-8 predominate in the preterm lung, and are increased in infants developing bronchopulmonary dysplasia, due to conversion of interleukin-877 by neutrophil serine proteases and thrombin. Processing of interleukin-8 provides an attractive therapeutic target to prevent development of bronchopulmonary dysplasia.

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Section: Methodsmentioning

confidence: 99%

Role of Serine Proteases in the Regulation of Interleukin-877 during the Development of Bronchopulmonary Dysplasia in Preterm Ventilated Infants

et al. 2014

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“…Interleukin 8 (IL-8/CXCL8) is a protein belonging to the chemokine family with a molecular weight of 8-9 kDa [38,39]. It consists of two polypeptides: Ser-IL-8 composed of 72 amino acids and Ala-IL-8 composed of 77 amino acids [40]. The gene encoding IL-8 is located on chromosome 4, on the 4q13.3 region.…”

Section: Snps Of Il-8 In Ramentioning

confidence: 99%

Proinflammatory Cytokines (IL-1, -6, -8, -15, -17, -18, -23, TNF-α) Single Nucleotide Polymorphisms in Rheumatoid Arthritis—A Literature Review

Koper-Lenkiewicz

Sutkowska

Wawrusiewicz‐Kurylonek

et al. 2022

IJMS

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Conducted studies highlight that a mixture of genetic and environmental factors is responsible for rheumatoid arthritis (RA) development. This study aimed to analyze the available literature for the relationship between, on the one hand, single-nucleotide polymorphisms (SNPs) in the proinflammatory cytokines genes interleukin-1 (IL-1), -6, -8, -15, -17, -18, and -23, and tumor necrosis factor-alpha (TNF-α), and on the other hand, RA susceptibility, severity, and patients’ response to applied treatment. The PubMed database was searched for sources. Preference was given to articles which were published within the past 20 years. Data indicate that the relationship between selected SNPs in proinflammatory cytokines genes and susceptibility to developing RA is inconclusive, and it depends on the ethnicity of the population. Although the allelic and genotypic frequencies of many SNPs in proinflammatory cytokines genes analyzed did not differ between RA patients and healthy controls, deeper analysis showed that these polymorphisms have a relationship with clinicopathological features of RA. SNPs in proinflammatory cytokines genes also “modify patients’ response” to applied treatment. Further studies, on larger cohorts of subjects and in different populations, should be conducted to elucidate the role of SNPs in IL-1, -6, -8, -15, -17, -18, and -23, and TNF-α genes in RA patients.

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“…In general, ELISA was performed as described previously (Nashkevich et al, 2002). Mouse monoclonal antibodies (B5 and A11) specific to natural human TNF-R p55 antigen, as well as mouse monoclonal antibodies N11 (specific to IL-877) and 4C (for use in the capture steps of the assay), and the clone products 3A and H6 (used for the latter detection steps) were provided by Nikolai N. Voitenok (Fund for Molecular Hematology & Immunology, Moscow, Russia).…”

Section: Elisa For Quantification Of Soluble Receptor Tnf P55 and Isomentioning

confidence: 99%

TNF receptor p55 and IL-8₇₂and IL-8₇₇isoforms: blood and urine levels in breast cancer patients

Shichkin¹,

Lon

Yugrinova

et al. 2009

Journal of Immunotoxicology

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A monoclonal antibody and an enzyme immunoassay for human Ala-IL-877

Cited by 5 publications

References 43 publications

Role of Serine Proteases in the Regulation of Interleukin-877 during the Development of Bronchopulmonary Dysplasia in Preterm Ventilated Infants

Role of Serine Proteases in the Regulation of Interleukin-877 during the Development of Bronchopulmonary Dysplasia in Preterm Ventilated Infants

Proinflammatory Cytokines (IL-1, -6, -8, -15, -17, -18, -23, TNF-α) Single Nucleotide Polymorphisms in Rheumatoid Arthritis—A Literature Review

TNF receptor p55 and IL-8₇₂and IL-8₇₇isoforms: blood and urine levels in breast cancer patients

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A monoclonal antibody and an enzyme immunoassay for human Ala-IL-877

Cited by 5 publications

References 43 publications

Role of Serine Proteases in the Regulation of Interleukin-877 during the Development of Bronchopulmonary Dysplasia in Preterm Ventilated Infants

Role of Serine Proteases in the Regulation of Interleukin-877 during the Development of Bronchopulmonary Dysplasia in Preterm Ventilated Infants

Proinflammatory Cytokines (IL-1, -6, -8, -15, -17, -18, -23, TNF-α) Single Nucleotide Polymorphisms in Rheumatoid Arthritis—A Literature Review

TNF receptor p55 and IL-872and IL-877isoforms: blood and urine levels in breast cancer patients

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TNF receptor p55 and IL-8₇₂and IL-8₇₇isoforms: blood and urine levels in breast cancer patients