A monoclonal antibody‐based indirect competitive enzyme‐linked immunosorbent assay to quantify swertiamarin and related compounds in <i>Swertia japonica</i> Makino

Nuntawong, Poomraphie; Horikawa, Tamaki; Ochi, Akihiro; Wada, Shinpei; Tsuneura, Yumi; Tanaka, Hiroyuki; Sakamoto, Seiichi; Morimoto, Satoshi

doi:10.1002/pca.2999

Cited by 3 publications

(1 citation statement)

References 32 publications

(35 reference statements)

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“…The enzyme-linked immunosorbent assay is applied to determine the serum CXCL10 and CXCL16 in the patient. [18][19][20] After admission, all MPP patients undergo bronchoscopy, and bronchoalveolar lavage fluid (BALF) is left to measure the MP-DNA replication rate. After imaging examination, based on the examination results, the patient's lung characteristics are recorded and observed for the presence of pleural effusion and other conditions.…”

Section: Methodsmentioning

confidence: 99%

Clinical characteristics and changes in serum CXCL10 and CXCL16 levels in patients with severe mycoplasma pneumonia

Zhang,

Ke,

Wang

et al. 2024

Medicine

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To explore the clinical characteristics and changes in serum CXCL10 and CXCL16 in patients with severe mycoplasma pneumonia, and to analyze the risk factors of severe mycoplasma pneumonia. About 258 children with acute mycoplasma pneumoniae pneumonia (MPP) admitted to the respiratory department of a certain hospital from January 2020 to December 2022 were selected as the study subjects. According to the severity of MPP, patients are divided into 2 groups, namely the mild illness group (Q group) and the severe illness group (Z group). The number of cases in these 2 groups of children is 167 and 91, respectively. The serum CXCL10, CXCL16, and other indicators of 2 groups are tested. Compared to group Q, patients in group Z have a higher proportion of extrapulmonary complications, longer cough time, longer shortness of breath, and longer wheezing time (P < .05). The serum CXCL16 is higher and the proportion of pleural effusion is higher (P < .01). There are more cases of fever, longer fever duration, longer hospital stay, higher serum CXCL10, and higher D-dimer levels (P < .001). The area under the curve of the probability curve for predicting severe mycoplasma pneumonia is 0.975 (P < .05). Children with severe mycoplasma pneumonia have significantly longer fever duration and hospital stay than those with mild symptoms. The serum levels of CXCL10 and CXCL16 are significantly elevated.

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Section: Methodsmentioning

confidence: 99%

Clinical characteristics and changes in serum CXCL10 and CXCL16 levels in patients with severe mycoplasma pneumonia

Zhang,

Ke,

Wang

et al. 2024

Medicine

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Development of a specific monoclonal antibody-based icELISA for detection of arecoline in traditional Chinese medicines and fresh areca nuts

Wang

Ding

et al. 2022

Food and Agricultural Immunology

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Activated Carbon-Based Immunochromatographic Strip Test for the Rapid Qualitative Analysis of Swertiamarin and Sweroside

Nuntawong

Horikawa

Tanaka

et al. 2022

Journal of AOAC INTERNATIONAL

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Background Swertia japonica (S. japonica) is a medicinal plant that belongs to the Gentianaceae family. Several reports confirm the biological effects of the S. japonica extract. This plant is mainly used as a digestive stimulant, appetite stimulant, and gastrointestinal disease remedy in Japan. Secoiridoid glycosides are a group of compounds related to the beneficial effects of this plant. Objective We developed an immunochromatographic strip test for major secoiridoid glycosides, such as swertiamarin (SM) and sweroside (SS) detection. Method We fabricated an immunoprobe using activated carbon as a reporter molecule and a monoclonal antibody against SM and SS (MAb D2) as a detection molecule. The test and control zones of the strip test contained SM-cBSA and Goat pAb anti-mouse IgM HRP conjugate, respectively. The immunoprobe reacted competitively with free SM and/or SS and immobilized SM-cBSA. The results were read and interpreted by the black spot intensity in the test zone. Results We succeeded in developing a strip test system with a detection limit (LOD) of 12.5 µg/mL. The selectivity and reliability evaluation revealed that the strip test is suitable for detecting SM and SS in S. japonica. The result was ready to be read in 30 min. Conclusion and highlights To the best of our knowledge, this is the first immunochromatographic strip test developed for the detection of SM and SS in S. japonica samples. This method can be a useful tool for the screening of biologically active S. japonica samples for further preparation of traditional medicine.

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A monoclonal antibody‐based indirect competitive enzyme‐linked immunosorbent assay to quantify swertiamarin and related compounds in Swertia japonica Makino

Cited by 3 publications

References 32 publications

Clinical characteristics and changes in serum CXCL10 and CXCL16 levels in patients with severe mycoplasma pneumonia

Clinical characteristics and changes in serum CXCL10 and CXCL16 levels in patients with severe mycoplasma pneumonia

Development of a specific monoclonal antibody-based icELISA for detection of arecoline in traditional Chinese medicines and fresh areca nuts

Activated Carbon-Based Immunochromatographic Strip Test for the Rapid Qualitative Analysis of Swertiamarin and Sweroside

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