A monoclonal antibody directed against the neurokinin-1 receptor contains a peptide sequence with similar hydropathy and functional properties to substance P, the natural ligand for the receptor

Antibody Complementarity-Determining Regions (CDRs) Can Display Differential Antimicrobial, Antiviral and Antitumor Activities

“…Several peptides including amino acids from the CDRs of anti-CD4 mAb ST40 and framework residues flanking the CDRs bound to soluble CD4 and displaced Ab binding [28]. Bioactive paratope-derived peptides of potential pharmacological interest were also deduced by hydropathic complementarity [29].…”

Hydropathic Complementarity Determines Interaction of Epitope 869HITDTNNK876 in Manduca sexta Bt-R1 Receptor with Loop 2 of Domain II ofBacillus thuringiensis Cry1A Toxins

“…Isolation of peptides that mimic natural ligands by phage display have been successful in mapping protein epitopes by searching amino acid sequence similarities (53). Nevertheless, as was pointed out before (39), the most reliable way to map binding epitopes is to search hydropathic profile similarities rather than amino acid sequences similarities. We propose that amino acid sequence similarities could also be searched considering both amino acid sequences of the coding and noncoding DNA strands of the interacting epitopes.…”

“…Among more than 40 examples (for review, see Ref. 34) hydropathic complementarity has been successfully applied to produce biologically active synthetic analogs of receptor binding sites (33,35,36) and ligands (37) and to map binding epitopes of natural ligands with their receptors (38,39). Also, hydropathic complementarity has been shown to determine several peptide-antibody interactions (40).…”

Role of complementary proteins in autoimmunity: an old idea re-emerges with new twists

“…Three main theories, proposed by Root-Bernstein and Holsworth [10], Siemion et al [11] and Smith et al [6], have been put forward to explain this proposed symmetry [5,[10][11][12][13][14][15][16]. All three hypotheses use ideas regarding amino acid interactions developed by Meckler [17] and all three have been exploited to design complementary peptides that can be used to block protein-ligand interactions [15,[18][19][20][21] or to design vaccines [5,9].…”