1987
DOI: 10.1099/00222615-23-2-163
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A monoclonal antibody directed against a serotype-specific, outer-membrane protein of Haemophilus influenzae type b

Abstract: Summary. Monoclonal antibodies (Mabs) were produced against outer-membrane proteins (OMPs) of Haemophilus infuenzae type b. The clones were screened by ELISA with outer-membrane preparations of H . inJuenzae type b and untypable strains as coating antigens. Antibodies directed against the proteins of mol. wt (lo3) 43, 37 and 13 were identified by immunoblotting of SDS-PAGE patterns of OMPs. Proteolytic enzyme treatments of the OMPs resulted in reduction of Mab reactivity as measured by ELISA. Furthermore, the … Show more

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Cited by 35 publications
(42 citation statements)
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“…pertussis S M E or LOS. Digestion of B. pertussis SME or LOS with proteinase K was performed as previously described (Hamel et al, 1987). The immune reactivity of mAbs with the enzyme-treated SME or LOS were thereafter tested by the dot-enzyme immunoassay as described above.…”
Section: Methodsmentioning
confidence: 99%
“…pertussis S M E or LOS. Digestion of B. pertussis SME or LOS with proteinase K was performed as previously described (Hamel et al, 1987). The immune reactivity of mAbs with the enzyme-treated SME or LOS were thereafter tested by the dot-enzyme immunoassay as described above.…”
Section: Methodsmentioning
confidence: 99%
“…The mouse mAb Hb-2 was previously characterized as recognizing a conformational, surface-exposed determinant on Hib porin [24]. To determine whether refolded Bac porin regenerated this conformational epitope, Hib porin, refolded Bac porin and misfolded Bac porin were tested by ELISA for their reactivity with mAb Hb-2 ( Fig.…”
Section: Reactivity Of Hb-2 To Refolded Bac Porinmentioning
confidence: 99%
“…influenzae antigens were prepared for immunization and immunoassays. The extraction of outer membranes from all H. influenzae isolates was performed by the lithium chloride method as described previously (Hamel et al, 1987~). Sarcosyl-insoluble preparations enriched in the major outer membrane proteins from non-typable H. influenzae MTL6 were prepared by the method described by Barenkamp et al (1981).…”
Section: Antigen Preparationsmentioning
confidence: 99%
“…Three days before the fusion, the mice were boosted with 1Opg P2 protein from non-typable H. influenzae 12085. Hybridomas were produced by fusion of splenic lymphocytes from immunized mice with non-secreting Sp2/0 myeloma cells as previously described (Hamel et al, 1987~). P2-protein-specific hybridomas were cloned by sequential limiting dilutions, expanded and frozen in liquid nitrogen.…”
Section: Production Of Mabsmentioning
confidence: 99%