A Monoclonal Antibody (HAN-PC1), Reacting with a Maturation Antigen on Plasma Cells

Human myeloma: Several subsets of circulating lymphocytes express plasma cell‐associated antigens

“…Suprisingly, at least 40% of this population was represented by T lympho- PCA-1, HAN-PC1 and TEC-TI0 MoAb have been tested by several authors in peripheral blood from healthy and pathological subjects. A cross-reaction with other hemopoietic cells and T cell lines has been described (10,15,16). The positivity of circulating lymphocytes that we observed has never been reported.…”

“…Monoclonal antibodies B1, B4, and PCA-1 were purchased from Coulter Clone, (Luton, England); Leu2, Leu3, Transferrin Receptor, CALLA, and phycoerythrin conjugated Leu2, Leu3, Leul5, HLA-DR from Becton and Dickinson (Mountain View, CA); OKT3 from Ortho Diagnostic (Raritan, NJ); TEC-T10 (15), that shows a very similar reactivity to OKTlO, was obtained from Technogenetics (Turin, Italy); HAN-PCl (16), recognizing a plasma cell-associated antigen, was kindly provided by Dr. Tidelmans. Fluorescein conjugated goat antimouse Ig was from Becton and Dickinson.…”

Identification of malignant plasma cell precursors in the bone marrow of multiple myeloma.

“…MAB reactivity was defined by staining cells with two-color IF using the MAB plus G-anti-M-IgG-FITC or R-anti-M-IgM-FITC together with R-or G-anti-human Ig isotype-TRITC. The morphology of reacting cells was studied with the APAAP technique (22). The TdT staining was performed as previously described (24).…”

“…In the present paper, we provide evidence that in advanced forms of plasma cell malignancies such as MM, stages II and III (9), and in plasma cell leukemia (PCL), cells of lymphoid morphology are present that express common acute lymphoblastic leukemia antigen (CALLA, CD10) and HLA-DR, but do not contain terminal deoxynucleotidyl transferase (TdT-) and are negative for both surface Ig (slg) and cytoplasmic Ig (cylg) as well as for B cell antigens CD [19][20][21][22]. When these CALLA',Igcells are separated with a fluorescence-activated cell sorter (FACS), and stimulated with TPA in vitro, the induced cells transform into plasma cells that synthesize the heavy and light chains displayed by the patients' myeloma cells.…”

Activation of murine 'T' lymphomas in the presence of a human myeloma cell line, RPMI-8226, in vivo

“…Electron micrographs of the cells show that they are plasmablastoid with a well developed endoplasmic reticulum (Figure la). They are hyperdiploid, exhibit lambda immunoglobulin on the cell surface as well as HAN PCI, which is characteristic of plasma cells (Mertens et al, 1985), and have a doubling time of 40 h in vitro (Miller & Bell, 1987).…”