A monoclonal antibody recognizing a determinant common to HLA—A3 and All

Billing, Ronald; Clark, Bill; Lucero, K.; Shi, Bing; Terasaki, Paul I.

doi:10.1111/j.1399-0039.1982.tb01427.x

Cited by 10 publications

(4 citation statements)

References 13 publications

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“…One of these involved immunization of mice using B-lymphoblastoid cell line typed HLA-A3, B7, DR4 [14] and the other, using acute myelogenous leukemia (AML) cells expressing HLA-A3 [10]. The present investigation was conducted to produce MAbs against HLA-A3 utilizing a third approach in which HLA-A3 antigen was puri- fied from human HLA-A3 positive leukocytes and used for the generation of specific antibodies.…”

Section: Discussionmentioning

confidence: 99%

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Production of mouse monoclonal antibodies against human HLA-A3 for use in microlymphocytotoxicity technique

Yari

Sareh

Sadri

et al. 2009

HAB

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Monoclonal antibody production using the hybridoma technology is one of the attractive areas of research. The aim of this study was to investigate the production of monoclonal antibodies to human HLA-A3 antigen for use in microlymphocytotoxicity technique. HLA-A3 antigen was purified from human leukocytes and injected to several mice. The spleen-derived cells of immunized animal were fused with P3-X63-Ag8 myeloma cells. Obtained hybridoma cells were grown in HAT medium and the supernatant of the cells were screened for antibodies using ELISA and microlymphocytotoxicity technique. Produced monoclonal antibody from the hybridoma clone, B1F10 showed the specificity for human HLA-A3. Furthermore, in this study, we encountered with clones of immortal hybrid cells with completely different features, they were adherent and non-producers for antibodies. This observation could imply the possible role of the antigen (HLA) and its nature in the expansion of cell types other than B cells in the spleen of mouse during immunization.

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Section: Discussionmentioning

confidence: 99%

“…For A3, the alpha, "A", chain is encoded by the HLA-A*03 allele group. Production of monoclonal antibodies to A3 antigen is of basic and clinical importance [10][11][12][13][14]. This antibody is among several antibodies that are used in serologic typing of HLA class I alleles.…”

Section: Introductionmentioning

confidence: 99%

Production of mouse monoclonal antibodies against human HLA-A3 for use in microlymphocytotoxicity technique

Yari

Sareh

Sadri

et al. 2009

HAB

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“…shared by several different allelic products , Legrand & Dausset 1973, 1974. The concept of shared or "public" determinants has been substantiated immunochemically (Schwartz et al 1979(Schwartz et al , 1980a(Schwartz et al , 1980b and by the use of monoclonal antibodies (Parham 1981, Parham & Brodsky 1981, Billing et al 1982, Ellis et al 1982, Haynes et al 1982). In addition, the determinants of the HLA-Bw4/ Bw6 antigen system are present on the same polypeptide as their associated HLA-B specificities (Ayres & Cresswell 1976, Ferrone et al 1978, Barnstable et al 1979.…”

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confidence: 99%

A F(ab')2 fragment blocking study of the HLA‐B14 antigen

Darke¹

1985

Tissue Antigens

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The HLA‐B14 antigen exists in two serologically distinct forms termed Bw64 and Bw65. These two B14 subgroups were studied using the ability of F(ab')2 fragments from HLA‐B14 and Bw6 antisera to block the binding of cytotoxic HLA antibodies directed towards the same or an adjacent antigenic site. The findings suggest that the B14 antigen consists of several antigenic determinants some of which are spatially distinct and others which are closely associated. A “common” determinant possessed by both B14 subtypes is proposed which is separate from a Bw64 (or Bw65) site. Additional determinants that B14 shares with B8, B18 and B38/B39 appear to be distinct from the Bw64/Bw65 site, variably associated with the B14 “common” determinant but closely adjoining the Bw6 antigen.

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“…Several miirine monoclonal antibodies recognizing polymorphic H L A determinants have been described (Betts & McKenzie 1982, Colornbani et al 1982. A number of these antibodies detect "crosc-reactive" determinants in that they recognize more than one HLA polymorphic specificity, such as anti-A2/B17 (McMichael et al 1980) and anti-A3/All (Billing et al 1982). However, several monoclonal antibodics have been described which exactly recognize specificities defined by conventional alloanti-HLA sera, including A 2 (Parham & Bodmer 1978, Parham & Brodsky 198 l), B7 (Brodsky et al 1979), B27 (Grumct et al 1981, Trapani ct al.…”

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confidence: 99%

A new polymorphic determinant distinct from but expressed concomitantly with an HLA‐Bw44 specificity

SPARROW,

MCKENZIE

1984

Tissue Antigens

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A new antigenic determinant defined by a monoclonal antibody (6.7) is described which displays restricted expression being present only o n the cells of the immunizing donor and his father and not on a panel of Over one hundred unrelated IILA-typed cells. The determinant segregated with the HLA haplotypes of the immunizing donor's family and was shown by immunochemical and capping studies to be located on a subpopulation of HLA molecules. Further capping and antibody-blocking studies using HLA-typing alloantisera revealed that the 6.7-reactive determinant was located on the same molecules that expressed the HLA-Bw41 specificity but was a separate epitope to the polymorphic HLA specificity.

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A monoclonal antibody recognizing a determinant common to HLA—A3 and All

Cited by 10 publications

References 13 publications

Production of mouse monoclonal antibodies against human HLA-A3 for use in microlymphocytotoxicity technique

Production of mouse monoclonal antibodies against human HLA-A3 for use in microlymphocytotoxicity technique

A F(ab')2 fragment blocking study of the HLA‐B14 antigen

A new polymorphic determinant distinct from but expressed concomitantly with an HLA‐Bw44 specificity

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