A Monoclonal Antibody That Reacts with Nonallelic Enzyme Glycoproteins

Abstract

One of six monoclonal antibodies raised against purified human placental alkaline phosphatase cross-reacts with the adult and fetal forms of intestinal alkaline phosphatase. The placental and intestinal enzymes are nonallelic. A new electrophoretic titration procedure was used to assess the relative reactivities of the different enzymes with the antibody. The placental enzyme was the most reactive. However, the adult intestinal enzyme showed greater reactivity than the fetal enzyme. The determinants to which the antibody binds on these three forms of alkaline phosphatase presumably differ in their detailed molecular configurations.

Human placental alkaline phosphatase in liver and intestine.

“…E 1. (12,13) may be due to the presence of the placental enzyme in purified preparations of intestine and liver ALPase. It will be interesting to see whether the other isozymes are also widely expressed.…”

Structural analysis of human adult and fetal alkaline phosphatases by cyanogen bromide peptide mapping.

“…In other studies, 11 monoclonal antibodies that react with both intestinal ALPases have been produced in this laboratory: 6 were raised to fetal intestinal ALPase (18), 1 to placental ALPase (19), and 4 to an intestinal-like ALPase found in the human cell line D98/AH-2 (20). Of these, 9 can discriminate between adult and fetal intestinal ALPases in binding studies.…”

Characterization of human foetal intestinal alkaline phosphatase. Comparison with the isoenzymes from the adult intestine and human tumour cell lines

“…Other investigations have also confirmed the existence of a foetal intestinal alkaline phosphatase that is distinctively different from the adult intestinal enzyme (Suzuki et al, 1975;Mulivor et al, 1978a;Miki et al, 1978;Gogolin et al, 1982). An alkaline phosphatase with electrophoretic mobility similar to that of foetal intestinal alkaline phosphatase has been observed in hepatoma cells (Suzuki Vol.…”