A monoclonal antibody to an antigen present on the microvillous membrane of the trophectoderm of the preimplantation blastocyst of the pig

Whyte, A.; Bacon, Martha; Ellis, Shirley A.

doi:10.1530/jrf.0.0710599

Cited by 16 publications

(7 citation statements)

References 16 publications

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“…The (Fig 7b)c and crude ultrastructural observations with respect to the apical, lat-*7c), which contains eral, and basal surfaces, the cell-cell communication, and ongly stimulate por the organization and types of cytoplasmic organelles in Jagborcine macrophages 1 cells correspond closely to those in the trophectoderm subsets as do mouse of preimplantation blastocysts [23][24][25]41] and trophoblastic tion show a striking vesicles [33] and even in the postimplantation placenta [21,22]. A monoclonal antibody (SN1/38) that reacts specifically to an antigen on the surface of trophectoderm cells of the e pig blastocyst, the pig was produced by Whyte et al [42]. Approximately 20% plete layer into the of the monolayer cells derived from trophoblastic vesicles ds do not become produced by Whyte et al [33] expressed the trophectoder- Maximum proliferation occurred at 50% Jag-1 CM.…”

Section: Discussionmentioning

confidence: 98%

A Porcine Trophoblast Cell Line that Secretes Growth Factors Which Stimulate Porcine Macrophages1

Ramsoondar

Christopherson

Guilbert

et al. 1993

Biology of Reproduction

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We have previously described experiments in both the mouse and the human indicating that cytokines capable of activating macrophages (colony-stimulating factor-1 [CSF-1], granulocyte-macrophage colony-stimulating factor [GM-CSF], and interleukin-3 [IL-3]) are produced by, and/or stimulatory of, trophoblast cells in these species. In contrast to the complex hemochorial placenta of the mouse and humans, the pig has a simple diffuse type of placenta, designated as epitheliochorial. To determine whether similar phenomena might not apply to the porcine pregnancy, we have isolated a cell line, designated Jag-1, from the trophoblastic tips of Day 14 porcine embryos. We report here that this cell line is cytokeratin-positive, vimentin-negative, and therefore of epidermal origin. It also shares various morphological characteristics with porcine trophoblast as demonstrated at both the light and electron microscopic levels. In addition, Jag-1 cells and primary trophoblast tissue from Day 14 blastocyst do not express classical major histocompatibility (MHC) class I and class II antigens, a unique feature of trophoblast in many species. To determine the ability of this cell line to produce cytokines, we have developed an assay for porcine macrophage growth factors that utilizes uptake of tritiated thymidine. This assay responds positively to recombinant bovine GM-CSF and, more importantly, detects a similar activity in supernatants of the porcine trophoblast cell line and of Day 14 blastocysts. Thus porcine trophoblast cells, like their murine and human counterparts, produce and potentially interact with lymphohematopoietic cytokines that are traditionally associated with macrophages.

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Section: Discussionmentioning

confidence: 98%

A Porcine Trophoblast Cell Line that Secretes Growth Factors Which Stimulate Porcine Macrophages1

Ramsoondar

Christopherson

Guilbert

et al. 1993

Biology of Reproduction

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“…This approach has also been successfully used in the pig [6,20,21,23,28,29,37,38]. Wianny et al [25] developed another immunosurgical method when using a monoclonal antibody SN1/38 specifically raised against porcine trophectodermal cells [50]. Other methods used include treatment with calcium ionophore [51], microsurgery [22,24,32,48] and a digestive method using 0.25% Trypsin/EDTA [30,31].…”

Section: Icm Isolationmentioning

confidence: 99%

Putative Embryonic Stem Cell Lines from Pig Embryos

Vacková

Ungrova

Lopes

2007

Journal of Reproduction and Development

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Abstract. Embryonic stem cells (ES cells)were first established in the mouse, and they represent a population of pluripotent, undifferentiated cells derived from early embryos that is capable of proliferating without any limitation in an undifferentiated state. These cells retain the ability to differentiate in vitro or in vivo into derivates of all three germ layers, and when injected into blastocysts, they can participate in the formation of all tissues, including gonads (germ-line chimeras). It is possible to transfect them with a gene of interest, and the resulting transgenic cell lines can also be used for production of chimeras. Unfortunately, mammalian germ-line chimeras that can carry an inserted gene into their progeny have only been produced in the mouse. Logically, before application of stem cell therapies into a human medicine, it is necessary to verify the efficiency and safety of these methods with an acceptable animal model. The pig is currently used as a very convenient animal for pre-clinical applications, and therefore establishment of porcine ES cell lines is highly needed; unfortunately, no convincing ES cell lines have been produced in this species (and other domestic animals) to date. In this article, we discuss the recent advances in this field, especially oriented on possible reasons and obstacles why derivation of porcine ES cell lines is still unsuccessful.

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“…The embryos were washed three times in DMEM. Day 7 ICMs were isolated by immunosurgery, using a monoclonal antibody (SN1/38) specifically raised against porcine trophectodermal cells (kindly provided by A. Whyte, Cambridge, UK [21]). Blastocysts were incubated for 1 h at 38 0 C in the presence of SN1/38 diluted 1: 20 in DMEM.…”

Section: Isolation Of Icms and Epiblastsmentioning

confidence: 99%

Proliferation and Differentiation of Porcine Inner Cell Mass and Epiblast in Vitro1

Wianny

Perreau

Reviers

1997

Biology of Reproduction

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The proliferation rate and differentiation state were investigated in porcine inner cell masses (ICMs) and epiblasts in vitro. ICMs isolated from early blastocysts (Day 7 of pregnancy) and epiblasts isolated from preelongated blastocysts (Day 11 of pregnancy) were cultured for up to 5 days in the presence of human leukemia inhibitory factor (hLIF) (1000 U/ml). The proliferation rate was evaluated by determination of the percentage of cells in S-phase. The differentiation state was determined by studying the expression of the stage-specific embryonic antigen-1 (SSEA-1), a marker for undifferentiated murine embryonic stem (ES) cells, and the expression of laminin and cytokeratins 8/18, markers of ES cell differentiation. The staining pattern showed that freshly collected Day 11 epiblasts appeared undifferentiated but rapidly lost this characteristic in vitro. A decrease in the proliferation rate was also observed during culture. This decrease was reduced in the presence of high concentrations of hLIF (optimal concentrations: 5000 U/ml). Conversely, treatment of Day 11 epiblast cells with retinoic acid, an agent known to induce differentiation in murine ES cells, caused a dramatic decrease in the proliferation rate in vitro. In contrast to Day 11 epiblasts, Day 7 ICMs expressed SSEA-1 in vitro and showed a higher proliferation rate (p < 0.01). However, their proliferation rate also decreased during culture and following trypsinization. These results indicate that the undifferentiated characteristics of Day 7 ICMs are more likely to be maintained in vitro than are those of Day 11 epiblasts, which are rapidly committed into early differentiation.

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A monoclonal antibody to an antigen present on the microvillous membrane of the trophectoderm of the preimplantation blastocyst of the pig

Cited by 16 publications

References 16 publications

A Porcine Trophoblast Cell Line that Secretes Growth Factors Which Stimulate Porcine Macrophages1

A Porcine Trophoblast Cell Line that Secretes Growth Factors Which Stimulate Porcine Macrophages1

Putative Embryonic Stem Cell Lines from Pig Embryos

Proliferation and Differentiation of Porcine Inner Cell Mass and Epiblast in Vitro1

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