2012
DOI: 10.1016/j.jchromb.2011.10.044
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A monolith purification process for virus-like particles from yeast homogenate

Abstract: Monoliths are an alternative stationary phase format to conventional particle based media for large biomolecules. Conventional resins suffer from limited capacities and flow rates when used for viruses, virus-like particles (VLP) and other nanoplex materials. The monolith structure provides a more open pore structure to improve accessibility for these materials and better mass transport from convective flow and reduced pressure drops. To examine the performance of this format for bioprocessing we selected the … Show more

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Cited by 53 publications
(47 citation statements)
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“…On the other hand, monoliths display a higher accessible surface area for large molecules and hence greater binding capacity [6,7]. Among the large molecules of interest viruses [8][9][10], virus-like particles [11], nucleic acids, and plasmid DNA [12][13][14] have been successfully isolated and purified by several research groups demonstrating the advantages of monolithic supports for these applications.…”
Section: Introductionmentioning
confidence: 99%
“…On the other hand, monoliths display a higher accessible surface area for large molecules and hence greater binding capacity [6,7]. Among the large molecules of interest viruses [8][9][10], virus-like particles [11], nucleic acids, and plasmid DNA [12][13][14] have been successfully isolated and purified by several research groups demonstrating the advantages of monolithic supports for these applications.…”
Section: Introductionmentioning
confidence: 99%
“…Nowadays, these columns can be replaced by monolithic chromatographic supports, whose main characteristics are extreme permeability that allows a very efficient mass transport at low back pressures, good separation efficiency that decreases relatively slowly with increasing flow velocity and separation at high flow rates. Due to such characteristics, monoliths are frequently used in chromatographic separations of biomolecules, organic acids and purification of viruses [28][29][30][31] while their use in speciation analysis is scarce. Examples of successful application of monolithic chromatography in speciation analysis are the investigations on Al speciation in human serum [32,33], Zn in human milk [34], Ni in tea infusions [35], the study of the distribution of cisplatin [36] in serum of cancer patients and the investigation of the kinetics of the interaction and distribution of Ptbased chemotherapeutics with proteins in blood serum [37].…”
Section: Introductionmentioning
confidence: 99%
“…Another main advantage of monoliths is their flow-independent DBC allowing straight-forward scalability [3,[26][27][28]. A direct comparison between monoliths and particle-based resins for the purification of virus-like particles from yeast homogenate has been conducted by Burden et al The authors concluded that the monoliths are three-fold superior in terms of DBC with equivalent recovery in yield (90%) compared to particle-based resins [29].…”
Section: Adsorption Area and Dynamic Binding Capacitymentioning
confidence: 99%
“…Burden et al tackled lipid fouling by sample pretreatment, using Amberlite/XAD-4 beads for lipid removal, which doubled the dynamic binding capacity of a monolithic disk column [29].…”
Section: Limitations Of Monolithic Columnsmentioning
confidence: 99%