A morphological, morphometric and histochemical study of the oviduct in pregnant and non‐pregnant females of the plains viscacha (<i><scp>L</scp>agostomus maximus</i>)

Flamini, Mirta Alicia; Barbeito, Claudio Gustavo; Portiansky, Enrique Leo

doi:10.1111/azo.12018

Cited by 14 publications

(10 citation statements)

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“…It is impossible to compare our results with others as the cycle effect on the oviductal infundibulum in other primates has not been investigated. The absence of fucose residues has also been observed in the oviductal infundibulum of porcine [ 49 ] and plains viscacha ( Lagostomus maximus ) [ 50 ]. Distinct OGP has been detected in the infundibulum of several mammals [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Modification of Morphology and Glycan Pattern of the Oviductal Epithelium of Baboon Papio hamadryas during the Menstrual Cycle

Desantis

Albrizio

Lacitignola

et al. 2022

Animals

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The mammalian oviduct is a highly specialized structure where fertilization and early embryonic development occur. Its mucosal epithelium is involved in maintaining and modulating a dynamic intraluminal fluid. The oviductal epithelium consists of ciliated and non-ciliated (secretory) cells whose differentiation and activity are sex hormone-dependent. In this study, we investigated for the first time both the morphology and the glycan composition of baboon oviductal epithelium during the menstrual cycle. Oviducts were laparoscopically removed from 14 healthy adult female Papio hamadryas whose menstrual cycle phase was assessed based on the sex hormone levels and the vaginal cytology features. Histological investigations were carried out on fimbriae, infundibulum, ampulla, and isthmus separately fixed in 4% (v/v) paraformaldehyde, embedded in paraffin wax, and stained with hematoxylin-eosin for morphological analyses and using a panel of nine fluorescent lectins for glycoconjugate characterization. The histomorphological analysis revealed that in the entire oviduct (i) the ciliated and non-ciliated cells were indistinguishable during the follicular and luteal phases, whereas they were highly differentiated during the preovulatory phase when the non-ciliated cells exhibited apical protrusions, (ii) the epithelium height was significantly higher in the preovulatory phase compared to other menstrual phases, and (iii) the number of ciliated cells significantly (p ≤ 0.05) increased from the fimbriae to the infundibulum and progressively reduced in the other oviductal segments with the lower presence of ciliated cells in the isthmus. The glycan characterization revealed a complex and region-specific composition during the different phases of the menstrual cycle. It can be summarized as follows: (i) high-mannosylated N-linked glycans (Con A reactivity) were present throughout the oviductal epithelium during the entire menstrual cycle and characteristically in the apical protrusions of non-ciliated cells of the ampulla during the preovulatory phase; (ii) sialoglycans with α2,3-linked sialic acids (MAL II binding) were expressed along the entire oviductal surface only during the preovulatory phase, whereas α2,6-linked ones (SNA affinity) were also detected in the surface of the luteal phase, although during the preovulatory phase they were characteristically found in the glycocalyx of the isthmus cilia, and O-linked sialoglycans with sialic acids linked to Galβl,3GalNAc (T antigen) (KsPNA) and terminal N-acetylgalactosamine (Tn antigen) (KsSBA) were found in the entire oviductal surface during all phases of the menstrual cycle; (iii) GalNAc terminating O-linked glycans (HPA staining) were mainly expressed in the entire oviducts of the luteal and preovulatory phases, and characteristically in the apical protrusions of the isthmus non-ciliated cells of the preovulatory phase; and (iv) fucosylated glycans with α1,2-linked fucose (LTA reactivity) occurred in the apical surface of fimbriae during the luteal phase, whereas α1,3/4-linked fucose (UEA I binders) were present in the apical protrusions of the ampulla non-ciliated cells and in the apical surface of isthmus during the preovulatory phase as well as in the isthmus apical surface of follicular-phase oviducts. These results demonstrate for the first time that morphological and glycan changes occur in the baboon oviductal epithelium during the menstrual cycle. Particularly, the sex hormone fluctuation affects the glycan pattern in a region-specific manner, probably related to the function of the oviductal segments. The findings add new data concerning baboons which, due to their anatomical similarity to humans, make an excellent model for female reproduction studies.

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Section: Discussionmentioning

confidence: 99%

Modification of Morphology and Glycan Pattern of the Oviductal Epithelium of Baboon Papio hamadryas during the Menstrual Cycle

Desantis

Albrizio

Lacitignola

et al. 2022

Animals

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“…El mismo mostró características similares a las descriptas para otros mamíferos, pudiendo diferenciarse sus regiones anatómicas (infundíbulo, ampolla, istmo y porción intramural). La presencia de numerosas flexuras lo asemejó al de los roedores múridos, rumiantes, équidos y suidos (Beck et al), así como a histricomorfos como la vizcacha de llanura (Lagostomus maximus) (Flamini et al, 2014), el puercoespín de cola de pincel (Atherurus africanus) (Mayor et al, 2003), el puercoespín crestado (Hystrix cristata) (Ozdemir, 2004) y la paca (Cuniculus paca) (Goncalves dos Reis et al, 2011).…”

Section: Discussionunclassified

Observaciones sobre la Morfología del Oviducto del Coipo (Myocastor coypus)

et al. 2015

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RESUMEN: Se efectuaron los análisis anatómico e histoarquitectónico del oviducto del coipo (Myocastor coypus) en la madurez sexual. Se trabajó con 34 oviductos que fueron segmentados en infundíbulo, ampolla, unión ámpulo-ístmica, istmo cefálico, medio y caudal y procesados con técnicas histológicas de rutina. Los oviductos se observaron como órganos tubulares y tortuosos, con amplia superficie infundibular, marcada flexura en la unión con los cuernos uterinos y el desarrollo de un colículo en la unión útero-tubárica. La mucosa presentó plegamientos que disminuyeron en número, grado de ramificación y altura desde la ampolla hasta los comienzos de la unión útero-tubárica. El epitelio de revestimiento estuvo compuesto por células ciliadas y células secretoras. En el infundíbulo y el istmo fue cilíndrico simple, en tanto que en la ampolla fue seudoestratificado cilíndrico. En las regiones caudales del istmo se observaron criptas revestidas por un epitelio cilíndrico de aspecto secretor. La lámina propia fue de tejido conectivo laxo y muy vascularizado. La túnica muscular incrementó su grosor y complejidad desde el infundíbulo a la unión útero-tubárica.PALABRAS CLAVE: Oviducto; Histología; Anatomía; Myocastor coypus. INTRODUCCIÓNEl coipo (Myocastor coypus) es un roedor neotropical perteneciente al suborden Histricomorpha, utilizado con finalidades zootécnicas en los países del MERCOSUR y de la Unión Europea. Existen investigaciones sobre la morfología general del aparato reproductor de M. coypus (Felipe et al., 1999), pero se carece de información específica sobre el oviducto. Las características morfológicas del oviducto han sido estudiadas en diferentes especies (Beck et al., 1974), lo cual favorece el desarrollo de investigaciones de su fisiología en distintas condiciones reproductivas y experimentales. El oviducto constituye un órgano de importancia anatómica y funcional para la reproducción y el desarrollo. Es el órgano que provee un ambiente óptimo en el cual las gametas son transportadas, ocurre la fecundación y comienza el desarrollo embrionario (Besenfelder et al., 2012). El objetivo del presente trabajo fue realizar el análi-sis morfológico, con perspectivas anatómica e histológica, del oviducto del coipo en la madurez sexual. MATERIAL Y MÉTODOSe trabajó con 34 oviductos de hembras vírgenes y sexualmente maduras de M. coypus obtenidos de un criadero con sistema a corral. Los animales fueron sacrificados por el método de zafra. El peso promedio de las mismas fue de 4,6±0,1 kg, con una edad de 8,1±1,3 meses. Previo a su fijación en líquido de Bouin, cada órgano fue segmentado en las siguientes regiones: infundíbulo, ampolla cefálica, media y caudal, unión ámpulo-ístmica, e istmo cefálico, medio, caudal e intramural. El procesamiento histológico se realizó con técnicas de rutina hasta su inclusión en parafina. Para determinar la histoarquitectura general se realizaron cortes seriados de 5 µm que fueron teñidos con hematoxilina y eosina, tricrómica de Mallory e impregnación argéntica. Para cada sector oviductal...

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“…The uterine tube is a part of female reproductive system which receives the Oocyte and provide a good situation for fertilization. The results showed that the uterine tube was tortuous, whitish to fleshy color and flexible organ with narrow lumen [7]. The uterine tube tortuousness is related to pre and reproductive life [8].…”

Section: A Morphological Measurementsmentioning

confidence: 99%

Histomorphological features of Rabbit Uterine Tube During Estrous Phases

Abdullah¹

2019

Tikrit J. Pure Sci.

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Microscopic features of the adult rabbit uterine tube during estrous cycle were studied. Twenty rabbit uterine tube were used. Rabbits were collected from local market. Sections from uterine tube infundibulum, ampulla and isthmus were prepared for histological study. Lengths and widths of various parts were obtained from the right tube. Ampulla formed the longest part, isthmus was narrowest segment and connected to the uterus. The infundibulum was with fimbrae. Epithelial liming was with few types of cells, and were; ciliated; non-ciliated secretory (peg cells) and basal cells were also demonstrated. The type of epithelial cells was pseudostratified epithelium. In the ampullary mucosa, large number of primary branches at the follicular phase was observed. Ciliation was more at the follicular phase than luteal phase. While secretory cells during follicular was less than that at luteal phase

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A morphological, morphometric and histochemical study of the oviduct in pregnant and non‐pregnant females of the plains viscacha (Lagostomus maximus)

Cited by 14 publications

References 31 publications

Modification of Morphology and Glycan Pattern of the Oviductal Epithelium of Baboon Papio hamadryas during the Menstrual Cycle

Modification of Morphology and Glycan Pattern of the Oviductal Epithelium of Baboon Papio hamadryas during the Menstrual Cycle

Observaciones sobre la Morfología del Oviducto del Coipo (Myocastor coypus)

Histomorphological features of Rabbit Uterine Tube During Estrous Phases

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