A morphometric study of early ovarian development in pouch young of the brushtail possum (Trichosurus vulpecula)

Shackell, G. H.; Norman, N.G.; McLeod, B. J.; Hurst, Philip

doi:10.1002/(sici)1097-0185(199610)246:2<224::aid-ar9>3.0.co;2-0

Cited by 6 publications

(2 citation statements)

References 15 publications

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“…This wide range of ages reflects the high degree of variation in the number of germ cells between animals and the method of estimating the ages of pouch young. Similar variation in the number of germ cells has been reported for possums [9] and other species [22,33]. The degree of variation was greatly reduced, however, when total germ cell numbers were normalized to ovarian volume.…”

Section: Discussionsupporting

confidence: 76%

“…Aspects of early gonadal development and follicular growth have been described in the tammar wallaby (Macropus eugenii) [2], bandicoots (Perameles nasuta and Isoodon macrourus) [3], gray short-tailed opossum (Monodelphis domestica) [4][5][6], and Virginia opossum (Didelphis virginiana) [7]. In the brushtail possum (Trichosurus vulpecula), various aspects of ovarian development have been reported [8][9][10][11]; however, these studies were limited by small numbers of animals and gaps in the ages studied. Therefore, one of the aims of the present study was to provide a more comprehensive account of key events during ovarian development in the brushtail possum.…”

Section: Introductionmentioning

confidence: 99%

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Gene Expression of the Tyrosine Kinase Receptor c-kit During Ovarian Development in the Brushtail Possum (Trichosurus vulpecula)1

Eckery

Lawrence

Juengel

et al. 2002

Biology of Reproduction

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Ovarian development and function have been extensively studied in eutherian species, with stem cell factor and its receptor, c-kit, having been shown to play key roles at various stages of these processes. In contrast, relatively little is known regarding ovarian development in marsupials. The aims of this study were, first, to establish the timing of key events during germ cell maturation and follicular development and, second, to determine the timing and cellular localization of gene expression for c-kit in the ovaries of a marsupial, the brushtail possum (Trichosurus vulpecula). For this study, ovaries were collected from possums ranging in age from Day 1 after birth to adult. Using stereology, the number of germ cells was found to increase rapidly during the first 60-100 days of life. This was followed by a sharp decline in number, wherein almost 90% of germ cells had disappeared by Day 180. From histological examinations, the time of initiation of meiosis, follicular formation, and follicular growth were determined to occur on Days 35, 50, and 60, respectively. Using in situ hybridization, c-kit gene expression was localized to germ cells and somatic cells during the first 15 days of life; however, after Day 30 and into adult life, c-kit expression was exclusive to germ cells. Results from this study suggest that the pattern of ovarian development is similar in marsupials to eutherians, and that c-kit may play a key role in germ cell development at various stages throughout life.

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Gene Expression of the Tyrosine Kinase Receptor c-kit During Ovarian Development in the Brushtail Possum (Trichosurus vulpecula)1

Eckery

Lawrence

Juengel

et al. 2002

Biology of Reproduction

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Ultrastructure of oogenesis in the brushtail possum

Frankenberg

Selwood

2001

Mol. Reprod. Dev.

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Marsupial ova are slightly larger on average than those of eutherians. They have often been described as “yolky”, although the nature and roles of their contents in later development are poorly understood. In the ova of all marsupial species studied, large quantities of electron‐lucent vesicles are present. This study describes the ultrastructure of oogenesis in the brushtail possum, Trichosurus vulpecula. Oocytes of primordial follicles contain a variety of organelles of unknown function, some of which have been described previously in bandicoots. Large electron‐lucent vesicles begin to accumulate after the transition from primordial follicle to primary follicle and are derived at least partly from the Golgi and endoplasmic reticulum. Other inclusions that are absent in some other marsupial species are present in growing possum oocytes in smaller quantities, including yolklike lipid bodies and electron‐dense granular bodies. Mol. Reprod. Dev. 58:297–306, 2001. © 2001 Wiley‐Liss, Inc.

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Stereological Assessment of Developing Mouse Ovarian Follicles in an in vitro Culture System

Chand¹,

Legge

2011

The Anatomical Record

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The aim of this project was to validate the use of ovarian follicular diameter measurement as an indicator of growth using stereology as an independent measure. A strong linear correlation was observed between follicular volume and diameter, and between follicular volume and the total number of granulosa cells. These results have provided a useful validation of a widely used technique for determining follicular growth in vitro.

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A morphometric study of early ovarian development in pouch young of the brushtail possum (Trichosurus vulpecula)

Cited by 6 publications

References 15 publications

Gene Expression of the Tyrosine Kinase Receptor c-kit During Ovarian Development in the Brushtail Possum (Trichosurus vulpecula)1

Gene Expression of the Tyrosine Kinase Receptor c-kit During Ovarian Development in the Brushtail Possum (Trichosurus vulpecula)1

Ultrastructure of oogenesis in the brushtail possum

Stereological Assessment of Developing Mouse Ovarian Follicles in an in vitro Culture System

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