A morphometric study on the longitudinal and lateral symmetry of the sural nerve in mature and aging female rats

Jeronimo, André; Jeronimo, Cláudia Além Domingues; Filho, Omar Andrade Rodrigues; Sanada, Luciana Sayuri; Fazan, Valéria Paula Sassoli

doi:10.1016/j.brainres.2008.05.055

Cited by 43 publications

(62 citation statements)

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“…The present data on fascicle size, and myelinated fibers number and density from both nerves are similar with other studies that used animals with distinct ages or other rat strains (Campos et al;Jeronimo et al, 2005Jeronimo et al, , 2008Knox et al, 1989;Saitua & Alvarez, 1988). For the Schwann cells, it is well known that they participate in the regeneration processes after a nerve injury.…”

Section: Discussionsupporting

confidence: 89%

“…All myelinated fibers present in the endoneural space were counted. Morphometric parameters of the fascicles and myelinated fibers of sural and saphenous nerves segments were obtained as described previously (Campos et al;Jeronimo et al, 2008).The total number of myelinated fibers and the total number of Schwann cell nuclei present in each transverse section of the fascicles were counted. The area and lesser diameter of each fascicle (excluding the perineurium) as well as each myelinated fiber (defined by the axon and its respective myelin sheath, excluding the Schwann cell nucleus when present) and respective axon (fiber excluding the myelin sheath) were measured with image analysis software (KS 400, Kontron 2.0, Eching Bei München, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…The myelinated fibers and Schwann cell nuclei densities were then calculated. The percentage of the total cross-sectional area of the endoneural space occupied by the myelinated fibers was calculated, and hereafter referred as the percentage of occupancy (Jeronimo et al, 2005(Jeronimo et al, , 2008Campos et al). For myelinated fibers, both axonal diameter and total fiber diameter were automatically measured.…”

Section: Methodsmentioning

confidence: 99%

“…In the more distal areas of the foot, the sural nerve is responsible for the sensory innervation of the lateral side and dorsal central area (Cobianchi et al, 2014) but it also contains a small percentage (4%) of motor fibers (Swett et al). The extensive use of the sural nerve in experimental investigations about degeneration and regeneration following nerve injury is described mainly from male rats (Unger et al, 1998) while the sural nerves from female rats was less investigated (Jeronimo et al, 2005(Jeronimo et al, , 2008.…”

Section: Introductionmentioning

confidence: 99%

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Hind Limb Sensory Innervation in Rats: Comparison between Sural and Saphenous Nerve Morphometry

et al. 2015

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NERI, L. O.; DE AMORIM, M. M.; CAMPOS, S. A. R.; SANADA, L. S. & FAZAN, V. P. S. Hind limb sensory innervation in rats:Comparison between sural and saphenous nerve morphometry. Int. J. Morphol., 33(2):743-750, 2015. SUMMARY:Although numerous studies investigate sensory recovery of the hind paw of the rat after nerve damage, still no comprehensive overview of its normal innervation is present in the literature. We investigated the morphometry of myelinated fibers in the sural and saphenous nerves and analyzed their size distributions in young rats. Six 30-day-old female Wistar rats were perfused with 2.5% glutaraldehyde and their right and left sural and saphenous nerves were prepared for light microscopy and morphometry. Morphometric data were compared between segments (proximal versus distal) and sides (right versus left) for the same nerves. Also, segments from right or left sides were compared between nerves (sural versus saphenous). Both, the sural and saphenous nerves, exhibited proximal to distal symmetry on both sides as well as left-right symmetry. Histograms of the diameter of the myelinated fibers were unimodal in both nerves, regardless of segments or sides with the peaks of the fibers size occurring between 2.5 and 4.0 µm. The axon distributions reflected the myelinated fiber distributions, with the sural and saphenous nerves peaking between 1.5 and 2.0 µm. The G ratio (the ratio between the axon and fiber diameters) distributions were also unimodal, with peaks at 0.6 for both nerves. This study contributes to the literature with information on the myelinated fibers morphometry from the two sensory nerves responsible for the rat hind limb innervation. This information is valuable for a better understanding of the possible contribution of collateral sprouting from the sural or saphenous nerves on the paw sensory territory recovery observed after one of these nerves is damaged.

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Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hind Limb Sensory Innervation in Rats: Comparison between Sural and Saphenous Nerve Morphometry

et al. 2015

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“…All myelinated fibers present in the endoneural space were counted. Morphometric parameters of the fascicles and myelinated fibers of sural nerve segments were obtained as described previously 6,8 . Briefly, the total number of myelinated fibers and the total number of Schwann cell nuclei present in each fascicle were counted.…”

Section: Methodsmentioning

confidence: 99%

Can Wistar rats be used as the normotensive controls for nerve morphometry investigations in spontaneously hypertensive rats (SHR)?

Sanada¹,

Tavares

Neubern³

et al. 2011

Acta Cir. Bras.

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PURPOSE:We compared the sural nerve morphology among Wistar (WR), Wistar-Kyoto (WKY) and Spontaneously hypertensive (SHR) rats, including the nerve fascicles and myelinated fibers morphometry. METHODS: Age matched (20 weeks) female WR (N=6), WKY (N=6) and SHR (N=7) had their right and left sural nerves removed, embedded in epoxy resin, and observed by light microscopy. Morphometric analysis was performed with the aid of computer software. RESULTS: Despite presenting the same age, WR were heavier than WKY and SHR, as were SHR compared to WKY. Systolic arterial pressure was higher in SHR compared to WR, but no differences between SHR and WKY or WR and WKY were observed. The sural nerves were morphometrically symmetric between proximal and distal segments on the same side and between sides in all strains with no differences in the myelinated fiber number. Schwann cell number and density were smaller in SHR and G ratio was larger in SHR, indicating that SHR have thinner myelinated fibers. CONCLUSION: Sural nerve morphology is similar between WKY and WR, allowing the use of WR as the SHR controls in morphological investigations involving peripheral neuropathies. Keywords: Sural Nerve. Rats, Inbred SHR. Rats, Inbred WKY. Nerve Fibers. Rats. RESUMO OBJETIVO:Comparar a morfologia do nervo sural em ratos Wistar (WR), Wistar Kyoto (WKY) e espontanemanete hipertensos (SHR), incluindo a morfometria dos fascículos e fibras mielínicas. MÉTODOS: Os nervos surais direito e esquerdo de WR (N=6), WKY (N=6) e SHR (N=7), com 20 semanas de idade foram removidos e preparados para inclusão em resina epóxi e microscopia de luz. A morfometria foi realizada com o auxílio de um programa de computador. RESULTADOS: Apesar de apresentarem a mesma idade, WR são mais pesados que os WKY e SHR. Ainda, SHR são mais pesados que os WKY. A pressão arterial sistólica foi significativamente maior nos SHR comparados aos WR, sem diferença entre WKY e SHR ou WR e WKY. Os nervos surais são morfometricamente simétricos entre segmentos proximal e distal e entre lados direto e esquerdo nas três diferentes linhagens, sem diferença no número de fibras mielínicas. O número e a densidade de células de Schwann foram menores e a razão G foi maior nos SHR, indicando a presença de fibras mielínicas com bainha mais fina. CONCLUSÃO: A morfologia do nervo sural é semelhante ente WR e WKY, permitindo o uso de WR como controles dos SHR nas investigações envolvendo neuropatias periféricas. Descritores: Nervo Sural. Ratos Endogâmicos SHR. Ratos Endogâmicos WKY. Fibras Nervosas. Ratos. Can Wistar rats be used as the normotensive controls for nerve morphometry investigations in spontaneously hypertensive rats (SHR)?Acta Cirúrgica Brasileira -Vol. 26 (6) 5011 -515

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Ultrastructural Morphology and Morphometry of Phrenic Nerve in Rats

Fazan

Filho

Jordão

et al. 2008

The Anatomical Record

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Despite numerous literature reports on the morphometry of the myelinated fibers of phrenic nerves in rats, a systematic study of the longitudinal and lateral symmetry of the unmyelinated fibers morphometry is not available. In this study, we have undertaken ultrastructural and morphometric studies of the phrenic nerve in adult rats, assessing two different levels (proximal and distal) from both right and left sides. Phrenic nerves of adult male Wistar rats were prepared for epoxy resin embedding and transmission electron microscopy. Morphometric analysis was performed with the aid of computer software, which took into consideration the unmyelinated fiber number, density, area, and diameter, as well as ratio between myelinated and unmyelinated fibers, and the percentage of the fascicular area occupied by the myelinated and unmyelinated fibers. Comparison of data from proximal and distal segments on the same side and from the same levels between sides was performed. Differences were considered significant when P < 0.05. The most important finding is that morphometric parameters of the phrenic nerve unmyelinated fibers in adult rats are both longitudinally and laterally symmetric. This study adds important morphometric information about the unmyelinated fibers of the phrenic nerves in adult rats for proximal and distal levels on both sides of the animal.

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A morphometric study on the longitudinal and lateral symmetry of the sural nerve in mature and aging female rats

Cited by 43 publications

References 22 publications

Hind Limb Sensory Innervation in Rats: Comparison between Sural and Saphenous Nerve Morphometry

Hind Limb Sensory Innervation in Rats: Comparison between Sural and Saphenous Nerve Morphometry

Can Wistar rats be used as the normotensive controls for nerve morphometry investigations in spontaneously hypertensive rats (SHR)?

Ultrastructural Morphology and Morphometry of Phrenic Nerve in Rats

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