A mouse mammary epithelial cell line permissive for highly efficient human adenovirus growth

Wu, Cheng Jun; Öberg, Daniel; Rashid, Asif; Gupta, Rajesh Kumar; Mignardi, Marco; Johansson, Staffan; Akusjärvi, Göran; Svensson, Christina

doi:10.1016/j.virol.2012.10.034

Cited by 5 publications

(13 citation statements)

References 19 publications

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“…2C). In agreement with our previous results (Wu et al, 2013), the burst size increased 3-4 fold between 48 and at 60 h pi (Fig. 2C).…”

Section: Efficient Virus Production In Hadv-2-infected Nmumg-t Cellssupporting

confidence: 94%

“…We have previously identified a mouse mammary epithelial cell line, NMuMG, that supports HAdV-2 replication to the same level as observed in commonly used and highly permissive human cell lines, like the lung carcinoma cancer cell line A549 (Wu et al, 2013). NMuMG cells form well-structured and contact-inhibited monolayers and are unable to establish tumours in immunodeficient mice (Hynes et al, 1985).…”

Section: Introductionmentioning

confidence: 82%

“…We have previously shown that HAdV-2 infected NMuMG cells are as efficient as human A549 cells in producing infectious viral progeny (Wu et al, 2013). PCR analysis of viral early (E1A) and late (L3) mRNA expression ( Fig.…”

Section: Efficient Virus Production In Hadv-2-infected Nmumg-t Cellsmentioning

confidence: 97%

“…We recently demonstrated that the Contents lists available at ScienceDirect journal homepage: www.elsevier.com/locate/yviro NMuMG cells in tissue culture exhibit an amazing capacity to support replication by a number of HAdV types (Wu et al, 2013). Since NMuMG cells are essentially non-tumorigenic after transplantation in mice (Hynes et al, 1985) the ability to analyse the oncolytic properties of HAdV required establishment of a tumorigenic NMuMG cell line.…”

Section: Establishment Of the Sv40 T-antigen-transformed Cell Line Nmmentioning

confidence: 99%

“…Preparation of mRNA from tissue culture cells, cDNA synthesis and PCR were performed as described before (Wu et al, 2013).…”

Section: Dna and Rna Preparation And Pcr Analysismentioning

confidence: 99%

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HAdV-2-suppressed growth of SV40 T antigen-transformed mouse mammary epithelial cell-induced tumours in SCID mice

Cao

et al. 2016

Virology

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Human adenovirus (HAdV) vectors are promising tools for cancer therapy, but the shortage of efficient animal models for productive HAdV infections has restricted the evaluation of systemic effects to mainly immunodeficient mice. Previously, we reported a highly efficient replication of HAdV-2 in a non-tumorigenic mouse mammary epithelial cell line, NMuMG. Here we show that HAdV-2 gene expression and progeny formation in NMuMG cells transformed with the SV40 T antigen (NMuMG-T cells) were as efficient as in the parental NMuMG cells. Injection of HAdV-2 into tumours established by NMuMG-T in SCID mice caused reduced tumour growth and signs of intratumoural lesions. HAdV-2 replicated within the NMuMG-T-established tumours, but not in interspersed host-derived tissues within the tumours. The specific infection of NMuMG-T-derived tumours was verified by the lack of viral DNA in kidney, lung or spleen although low levels of viral DNA was occasionally found in liver.

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“…2C). In agreement with our previous results (Wu et al, 2013), the burst size increased 3-4 fold between 48 and at 60 h pi (Fig. 2C).…”

Section: Efficient Virus Production In Hadv-2-infected Nmumg-t Cellssupporting

confidence: 94%

Section: Introductionmentioning

confidence: 82%

Section: Efficient Virus Production In Hadv-2-infected Nmumg-t Cellsmentioning

confidence: 97%

Section: Establishment Of the Sv40 T-antigen-transformed Cell Line Nmmentioning

confidence: 99%

“…Preparation of mRNA from tissue culture cells, cDNA synthesis and PCR were performed as described before (Wu et al, 2013).…”

Section: Dna and Rna Preparation And Pcr Analysismentioning

confidence: 99%

See 3 more Smart Citations

HAdV-2-suppressed growth of SV40 T antigen-transformed mouse mammary epithelial cell-induced tumours in SCID mice

Cao

et al. 2016

Virology

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Expression of human CD46 and trans-complementation by murine adenovirus 1 fails to allow productive infection by a group B oncolytic adenovirus in murine cancer cells

Lei-Rossmann¹,

Jacobus²,

Taverner³

et al. 2018

j. immunotherapy cancer

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BackgroundOncolytic viruses are currently experiencing accelerated development in several laboratories worldwide, with some forty-seven clinical trials currently recruiting. Many oncolytic viruses combine targeted cytotoxicity to cancer cells with a proinflammatory cell lysis. Due to their additional potential to express immunomodulatory transgenes, they are also often known as oncolytic viral vaccines. However, several types of oncolytic viruses are human-specific and the lack of suitable immune-competent animal models complicates biologically relevant evaluation of their vaccine potential. This is a particular challenge for group B adenoviruses, which fail to infect even those immunocompetent animal model systems identified as semi-permissive for type 5 adenovirus. Here, we aim to develop a murine cell line capable of supporting replication of a group B oncolytic adenovirus, enadenotucirev (EnAd), for incorporation into a syngeneic immunocompetent animal model to explore the oncolytic vaccine potential of group B oncolytic viruses.MethodsTransgenic murine cell lines were infected with EnAd expressing GFP transgene under replication-independent or -dependent promoters. Virus mRNA expression, genome replication, and late protein expression were determined by qRT-PCR, qPCR, and immunoblotting, respectively. We also use Balb/c immune-competent mice to determine the tumourogenicity and infectivity of transgenic murine cell lines.ResultsOur results show that a broad range of human carcinoma cells will support EnAd replication, but not murine carcinoma cells. Murine cells can be readily modified to express surface human CD46, one of the receptors for group B adenoviruses, allowing receptor-mediated uptake of EnAd particles into the murine cells and expression of CMV promoter-driven transgenes. Although the early E1A mRNA was expressed in murine cells at levels similar to human cells, adenovirus E2B and Fibre mRNA expression levels were hampered and few virus genomes were produced. Unlike previous reports on group C adenoviruses, trans-complementation of group B adenoviruses by co-infection with mouse adenovirus 1 did not rescue replication. A panel of group B adenoviruses expressing individual mouse adenovirus 1 genes were also unable to rescue EnAd replication.ConclusionTogether, these results indicate that there may be major differences in the early stages of replication of group C and B adenoviruses in murine cells, and that the block to the life cycle of B adenoviruses in murine cells occurs in the early stage of virus replication, perhaps reflecting poor activity of Ad11p E1A in murine cells.Electronic supplementary materialThe online version of this article (10.1186/s40425-018-0350-x) contains supplementary material, which is available to authorized users.

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Infectivity of human group B adenovirus to cells from different species

Liu¹,

Mengke

Yuanqing³

et al. 2023

Preprint

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Adenovirus is a world public health problem that causes symptoms of respiratory, intestine, urethra and bladder, eyes and liver. The aim of this study is to observe the infectivity of adenovirus to cells from different species, which will provide evidence for finding potential adenovirus infecting animals and nature hosts. 10 cells from different species were infected with human adenovirus (HAdV) in group B (HAdV-3, HAdV-7, HAdV-14 and HAdV-55). Results showed that all the cells supported HAdV-55 entry and hexon protein expression. Increased viral DNA levels were observed in HEp-2, Vero, MDCK, PK15, OAR-L1, MDBK, and F81 cells, while not in CHO, NIH3T3, CT26WT and BHK21 cells. Infectious viral particles were produced in HAdV-55 infected HEp-2, Vero, MDCK, MDBK and PK15 cells, and in HAdV-7 infected HEp-2, Vero, MDCK, F81, MDBK, PK15 and OAR-L1 cells. Conclusively, it is possible to find animals that might be infected by human adenovirus through screening of susceptible cells, and the prevalence of human adenovirus in these animals deserves further investigation.

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A mouse mammary epithelial cell line permissive for highly efficient human adenovirus growth

Cited by 5 publications

References 19 publications

HAdV-2-suppressed growth of SV40 T antigen-transformed mouse mammary epithelial cell-induced tumours in SCID mice

HAdV-2-suppressed growth of SV40 T antigen-transformed mouse mammary epithelial cell-induced tumours in SCID mice

Expression of human CD46 and trans-complementation by murine adenovirus 1 fails to allow productive infection by a group B oncolytic adenovirus in murine cancer cells

Infectivity of human group B adenovirus to cells from different species

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