A Mouse Model of Salmonella Typhi Infection

Mathur, Ramkumar; Oh, Hee-Kyun; Zhang, Dekai; Park, Sung‐Gyoo; Seo, Jin Jong; Koblansky, A. Alicia; Hayden, Matthew S.; Ghosh, Sankar

doi:10.1016/j.cell.2012.08.042

Cited by 190 publications

(148 citation statements)

References 36 publications

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“…Consistent with this idea, a recent study suggests that inactivation of the murine Toll-like receptor 11 (TLR11), which is absent in humans, renders mice susceptible to S. Typhi infection (Mathur et al 2012). TLR11-deficient mice thus represent a potential model to study aspects of typhoid fever pathogenesis (Fang and Bäumler 2012).…”

Section: Humanized Micementioning

confidence: 84%

Host Specificity of Bacterial Pathogens

Bäumler

Fang

2013

Cold Spring Harbor Perspectives in Medicine

173

134

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Section: Humanized Micementioning

confidence: 84%

Host Specificity of Bacterial Pathogens

Bäumler

Fang

2013

Cold Spring Harbor Perspectives in Medicine

173

134

View full text Add to dashboard Cite

“…Another difference is the expression of TLR-11 in murine macrophages, which recognizes Salmonella flagellin. Human macrophages do not express TLR-11, which has already be shown to have a dramatic impact on Salmonella infection (30).…”

Section: Discussionmentioning

confidence: 99%

Direct measurement of oxidative and nitrosative stress dynamics in Salmonella inside macrophages

Heijden

Bosman

Reynolds

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Many significant bacterial pathogens have evolved virulence mechanisms to evade degradation and exposure to reactive oxygen (ROS) and reactive nitrogen species (RNS), allowing them to survive and replicate inside their hosts. Due to the highly reactive and short-lived nature of ROS and RNS, combined with limitations of conventional detection agents, the mechanisms underlying these evasion strategies remain poorly understood. In this study, we describe a system that uses redox-sensitive GFP to nondisruptively measure real-time fluctuations in the intrabacterial redox environment. Using this system coupled with high-throughput microscopy, we report the intrabacterial redox dynamics of Salmonella enterica Typhimurium (S. Typhimurium) residing inside macrophages. We found that the bacterial SPI-2 type III secretion system is required for ROS evasion strategies and this evasion relies on an intact Salmonella-containing vacuole (SCV) within which the bacteria reside during infection. Additionally, we found that cytosolic bacteria that escape the SCV experience increased redox stress in human and murine macrophages. These results highlight the existence of specialized evasion strategies used by intracellular pathogens that either reside inside a vacuole or "escape" into the cytosol. Taken together, the use of redox-sensitive GFP inside Salmonella significantly advances our understanding of ROS and RNS evasion strategies during infection. This technology can also be applied to measuring bacterial oxidative and nitrosative stress dynamics under different conditions in a wide variety of bacteria.oxidative stress | reactive oxygen species | Salmonella | bacterial pathogenesis | redox-sensitive GFP

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“…Following flagellin recognition, LPDCs induce the differentiation of naïve B cells into IgA-producing plasma cells and promote the differentiation of naïve T cells into IL-17-producing helper T cells (T H 17) and T helper type 1 (T H 1) cells ). TLR11 shares a close homology with TLR5 and recognizes flagellin independently of TLR5 (Mathur et al 2012). It also recognizes an unknown proteinaceous component of uropathogenic Escherichia coli (UPEC) (Zhang et al 2004) and a profilin-like molecule derived from Toxoplasma gondii (Yarovinsky et al 2005).…”

Section: Cd11bmentioning

confidence: 99%