A mPEG-PLGA-b-PLL copolymer carrier for adriamycin and siRNA delivery

Liu, Peifeng; Yu, Hui; Sun, Ying; Zhu, Mingjie; Duan, Yourong

doi:10.1016/j.biomaterials.2012.02.041

Cited by 131 publications

(83 citation statements)

References 46 publications

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“…Cellular reactions to NLC system were investigated with some modification of the technique determined for cytotoxicity measurement of ALA-NLC aqueous dispersion using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazonium bromide); MTT] assay (Lai & Hsieh, 2012;Liu et al, 2012;Wang et al, 2012b). HaCaT cells with a density of 5 Â 10 3 cells/well were seeded into 96-well plates and incubated for 24 h to endow cell attachment.…”

Section: Determination Of Cell Viabilitymentioning

confidence: 99%

Alpha-lipoic acid-loaded nanostructured lipid carrier: sustained release and biocompatibility to HaCaT cellsin vitro

Wang

Xia²

2013

Drug Delivery

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ALA-loaded nanostructured lipid carrier (ALA-NLC) was designed to improve physicochemical stability and water solubility, and promote sustained release of ALA as well as determine the biocompatibility of ALA-NLC. The ALA-NLC manufactured using hot high-pressure homogenization technique was investigated in terms of size, zeta potential, FTIR analysis and release behavior. In vitro cytotoxicity and biocompatibility were determined by incubating with HaCaT cells using the MTT assay, HE staining and Hoechst 33342 staining. Cell behavior and cellular division of HaCaT cells untreated and treated by ALA-NLC were investigated in real-time images gathered using time-lapse imaging system. The release investigation illustrated that only 6.9% of ALA released in 30 min from ALA-NLC formation, whereas it was 30.3% in free ALA system. ALA-NLC possessed a satisfactory release behavior of sustained release up to 72 h. It showed that ALA-NLC did not exert hazardous effect on HaCaT cells up to 81.9 mg/L without morphological alterations, revealing a satisfactory biocompatibility. Evidence was provided from time-lapse imaging system that cell behavior and cellular division of ALA-NLC treated HaCaT cells were in accordance with the control. These results of this investigation demonstrated that NLC encapsulated ALA formation (ALA-NLC) can improve stability, solubility and release of ALA; ALA-NLC was biocompatible to HaCaT cells.

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Section: Determination Of Cell Viabilitymentioning

confidence: 99%

Alpha-lipoic acid-loaded nanostructured lipid carrier: sustained release and biocompatibility to HaCaT cellsin vitro

Wang

Xia²

2013

Drug Delivery

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“…For the synthesis of mPEG-PDLLA-Phe-N BOC 32,33 ( Figure 1C), mPEG-PDLLA (10.0 g) and Phe-N BOC (4.0 g) were dissolved in 80 mL of cold CH 2 Cl 2 in a predried flask. Subsequently, DCC (3.0 g) and DMAP (0.1 g) were added into the flask for esterification at 0°C under protection of a N 2 stream.…”

Section: N Bocmentioning

confidence: 99%

Electrostatic interactions between polyglutamic acid and polylysine yields stable polyion complex micelles for deoxypodophyllotoxin delivery

Wang

Huang

Shen

et al. 2017

IJN

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To achieve enhanced physical stability of poly(ethylene glycol)-poly( d , l -lactide) polymeric micelles (PEG-PDLLA PMs), a mixture of methoxy PEG-PDLLA-polyglutamate (mPEG-PDLLA-PLG) and mPEG-PDLLA-poly( l -lysine) (mPEG-PDLLA-PLL) copolymers was applied to self-assembled stable micelles with polyion-stabilized cores. Prior to micelle preparation, the synthetic copolymers were characterized by 1 H-nuclear magnetic resonance (NMR) and infrared spectroscopy (IR), and their molecular weights were calculated by 1 H-NMR and gel permeation chromatography (GPC). Dialysis was used to prepare PMs with deoxypodophyllotoxin (DPT). Transmission electron microscopy (TEM) images showed that DPT polyion complex micelles (DPT-PCMs) were spherical, with uniform distribution and particle sizes of 36.3±0.8 nm. In addition, compared with nonpeptide-modified DPT-PMs, the stability of DPT-PCMs was significantly improved under various temperatures. In the meantime, the pH sensitivity induced by charged peptides allowed them to have a stronger antitumor effect and a pH-triggered release profile. As a result, the dynamic characteristic of DPT-PCM was retained, and high biocompatibility of DPT-PCM was observed in an in vivo study. These results indicated that the interaction of anionic and cationic charged polyionic segments could be an effective strategy to control drug release and to improve the stability of polymer-based nanocarriers.

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“…Boc-protection was removed by the addition of an excess amount of TFA [23]. Unreacted TFA was removed by addition of DCM to form positive azeotrope, which could be removed at a temperature much lower than the boiling point of TFA ( 72.4°C) [24].…”

Section: Synthesis and Characterization Of Plga-dab Conjugatementioning

confidence: 99%

Development and Analysis of Functionalized Poly(Lactide- Co-Glycolide)Polymer for Drug Delivery

Chen¹,

Huda²,

Benson³

2017

SOJPPS

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Functionalized polymer molecules are the backbone for formulation of targeted biomolecule delivery systems. We report here an effective method for the chemical synthesis of poly (lactide-co-glycolide)-1,4 diaminobutane (PLGA-DAB) in a controlled manner and an efficient gel permeation chromatography (GPC) method for analysis of functionalized PLGA. The synthetic method is simple, reproducible and the functionalized PLGA can be conjugated with any carboxylic acid-containing ligand and ultimately used for targeted drug delivery. The developed GPC analytical method is accurate, selective, precise and easily adaptable for analysing any other functionalized polymer with an amine group. These techniques provide an important basis for formulation scientists to develop functionalized polymers for targeted nanoparticle drug delivery.

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A mPEG-PLGA-b-PLL copolymer carrier for adriamycin and siRNA delivery

Cited by 131 publications

References 46 publications

Alpha-lipoic acid-loaded nanostructured lipid carrier: sustained release and biocompatibility to HaCaT cellsin vitro

Alpha-lipoic acid-loaded nanostructured lipid carrier: sustained release and biocompatibility to HaCaT cellsin vitro

Electrostatic interactions between polyglutamic acid and polylysine yields stable polyion complex micelles for deoxypodophyllotoxin delivery

Development and Analysis of Functionalized Poly(Lactide- Co-Glycolide)Polymer for Drug Delivery

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