“…However, to achieve detectable and reliable signals, these methods require 200–400 NTS per well and are limited to end-point analyses that do not provide any information on temporal evolution, which can be captured in impedance-based measurements ( Peak et al., 2010 ; Panic et al., 2015b ; Aguiar et al., 2017 ). Finally, automated image-acquisition systems have been proposed for enabling high-throughput and quantitative analysis of schistosomula phenotypes during drug exposure assays ( Lee et al., 2012 ; Chen et al., 2020 ). By tracking the temporal evolution of multiple phenotypic aspects, such as parasite morphology, size, and movements, these methods have the potential to produce high-dimensional data with additional information on drug action to support compound optimization, which is most likely missed by monitoring motility alone.…”