A multi-faceted approach for assessing evolutionary significant conservation units in the endangered Omphalodes littoralis subsp. gallaecica (Boraginaceae)

López, Lúa; Retuerto, Rubén; Roiloa, Sergio R.; Santiso, Xabier; Barreiro, Rodolfo

doi:10.1016/j.ppees.2014.09.003

Cited by 9 publications

(8 citation statements)

References 75 publications

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“…Zhao et al (2014) observed high genetic diversity when evaluating germplasm of 20 plants of Lilium (Liliaceae), with an average of 17-22 amplified bands per primer, but a lower I (0.35). Lopez et al (2015) evaluated genetic diversity in Omphalodes littoralis subsp gallaecica (Boraginaceae) with AFLP markers, which are also dominant, and observed heterozygosity of 0.356 and moderate genetic diversity.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

et al. 2016

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ABSTRACT. Varronia curassavica Jacq. is a medicinal and aromatic plant from Brazil with significant economic importance. Studies on genetic diversity in active germplasm banks (AGB) are essential for conservation and breeding programs. The aim of this study was to analyze the genetic diversity of V. curassavica accessions of the AGB of Medicinal and Aromatic Plants of the Federal University of Sergipe (UFS), using inter-simple sequence repeat molecular markers. Twentyfour primers were tested, and 14 were polymorphic and informative, resulting in 149 bands with 97.98% polymorphism. The UPGMA dendrogram divided the accessions into Clusters I and II. Jaccard similarity coefficients for pair-wise comparisons of accessions ranged between 0.24 and 0.78. The pairs of accessions VCUR-001/VCUR-503, VCUR-001/VCUR-504, and VCUR-104/VCUR-501 showed relatively low similarity (0.24), and the pair of accessions VCUR-402/VCUR-403 showed medium similarity (0.78). Twenty-eight accessions were divided into three distinct clusters, according to the STRUCTURE analysis. The genetic diversity of V. curassavica in the AGB of UFS is low to medium, and it requires expansion. Accession VCUR-802 is the most suitable for selection in breeding program of this species, since it clearly represents all of the diversity present in the AGB.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

et al. 2016

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“…ESUs are defined by lineage divergence, geographical isolation and ecological adaptive variation (Guia and Saitoh 2007). By combining genetic structure with appropriate assessments of ecological adaptive variation, it is possible to designate specific conservation units and optimize conservation management for endangered species (Lopez et al 2015, Torres-Cambas et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Identifying evolutionarily significant units for conservation of the endangered Malus sieversii using genome‐wide RADseq data

Zhang

2018

Nordic Journal of Botany

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Malus sieversii, a wild progenitor of the domesticated apple, is an endangered species and is assigned second conservation priority by the China Plant Red Data Book. It is urgent to carry out in situ conservation of this species, but previous studies have not identified evolutionarily significant units (ESUs) for conservation management. In this study, we investigated the genetic diversity and relationships of six M. sieversii populations from China using integrated analysis of microsatellite (nSSR) data, genome‐wide SNPs and previous results in order to propose a reasonable conservation management. The results showed that levels of genetic diversity were inconsistently reflected by our nSSR and previous studies, suggesting that indices of genetic diversity are not effective to identify priority conservation areas for M. sieversii. Based on the selection criteria of ESUs for endangered species conservation, ESUs should reflect lineage divergence, geographical separation and different adaptive variation. Our phylogenetic tree based on genome‐wide SNPs yielded a clear relationship of divergent lineages among M. sieversii populations, leading to new different from those of previous studies. Three independent lineages, including the pairs of populations Huocheng‐Yining, Gongliu‐Xinyuan and Tuoli‐Emin, were identified. The geographic distances between populations among the different phylogenetic lineages were much greater than those within the same phylogenetic lineage. A cluster analysis on environmental variables showed that the three independent lineages inhabit different environmental conditions, suggesting that they may have adapted to different environments. Based on the results, we propose that three independent ESUs should be recognized as conservation units for M. sieversii in China.

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“…From a conservation perspective, a selfing mating system suggests that conserving genetic diversity within a species requires conservation of different populations, whereas an outcrossing population with high gene flow could contain a high proportion of the diversity present in a species (Nybom, 2004; de Paz and Caujapé‐Castells, 2013). Lopez et al (2015) detected similar patterns of diversity in molecular markers and putatively adaptive phenotypic traits, with higher diversity among than within the small, fragmented populations of the endangered SC annual species Omphalodes littoralis subsp. gallaecica (Boraginaceae).…”

Section: Discussionmentioning

confidence: 87%

Genome‐wide genotyping estimates mating system parameters and paternity in the island species Tolpis succulenta

Gibson

Crawford

Holder

et al. 2020

American J of Botany

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Premise The mating system has profound consequences, not only for ecology and evolution, but also for the conservation of threatened or endangered species. Unfortunately, small populations are difficult to study owing to limits on sample size and genetic marker diversity. Here, we estimated mating system parameters in three small populations of an island plant using genomic genotyping. Although self‐incompatible (SI) species are known to often set some self‐seed, little is known about how “leaky SI” affects selfing rates in nature or the role that multiple paternity plays in small populations. Methods We generalized the BORICE mating system program to determine the siring pattern within maternal families. We applied this algorithm to maternal families from three populations of Tolpis succulenta from Madeira Island and genotyped the progeny using RADseq. We applied BORICE to estimate each individual offspring as outcrossed or selfed, the paternity of each outcrossed offspring, and the level of inbreeding of each maternal plant. Results Despite a functional self‐incompatibility system, these data establish T. succulenta as a pseudo‐self‐compatible (PSC) species. Two of 75 offspring were strongly indicated as products of self‐fertilization. Despite selfing, all adult maternal plants were fully outbred. There was high differentiation among and low variation within populations, consistent with a history of genetic isolation of these small populations. There were generally multiple sires per maternal family. Twenty‐two percent of sib contrasts (between outcrossed offspring within maternal families) shared the same sire. Conclusions Genome‐wide genotyping, combined with appropriate analytical methods, enables estimation of mating system and multiple paternity in small populations. These data address questions about the evolution of reproductive traits and the conservation of threatened populations.

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A multi-faceted approach for assessing evolutionary significant conservation units in the endangered Omphalodes littoralis subsp. gallaecica (Boraginaceae)

Cited by 9 publications

References 75 publications

Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

Identifying evolutionarily significant units for conservation of the endangered Malus sieversii using genome‐wide RADseq data

Genome‐wide genotyping estimates mating system parameters and paternity in the island species Tolpis succulenta

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