A multi‐laboratory evaluation of in vitro platelet assays: the tests for extent of shape change and response to hypotonic shock. Biomedical Excellence for Safer Transfusion Working Party of the International Society of Blood Transfusion

Abstract

Multiple laboratories were able to obtain comparable results with the ESC and HSR tests. They were able to show that the tests can be performed in an accurate, reproducible manner and with acceptable sensitivity.

Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions

“…25 The room temperature-stored PLTs maintained a HSR higher than 40% for the entire storage period (Fig. 3C).…”

In vitro Quality of Platelets with Low Plasma Carryover Treated with Ultraviolet C Light for Pathogen Inactivation

“…Similarly, the HSR has been shown to be negatively affected by PI treatment with other systems [10,11]. This effect is of particular interest given that the HSR is one of the few in vitro parameters thought to be associated with post-transfusion efficacy [21]. The increased externalization of phosphatidylserine expression and formation of microparticles is suggestive of membrane asymmetry, which may contribute to the reduced membrane responsiveness to osmotic stress.…”

Riboflavin and UV light treatment of platelets: a protective effect of platelet additive solution?

“…CD62P expression and annexin A5 binding were analyzed by flow cytometry as described elsewhere. 12 The hypotonic shock response (HSR) was performed according to Holme and coworkers 15 using an aggregometer (CH 490-4D, Chronolog, Havertown, PA). Plasma was used to dilute samples to prevent PAS-induced differences in the HSR result.…”