This research investigated bimetallic NiCu/HZSM-5 catalyst for the production of bio jet fuel via hydrotreating process. The catalysts with different Ni and Cu ratio were characterized by XRD, NH3-TPD, H2-TPR, SEM-EDX and N2-physisorption techniques. Palm fatty acid distillate (PFAD), nonedible by-product from palm oil refining process, was employed as a low-cost feedstock. For catalyst screening, the reaction was performed in an autoclave reactor under batch operation with operating temperature of 375?C, initial H2 pressure of 50 bars and reaction time of 3 h. The results showed that doping Cu could enhance deoxygenation activity while carbon formation was found to be suppressed. 12.5 wt.% Ni and 2.5wt.%Cu doping on HZSM-5 catalyst was found as a suitable catalyst to obtain a high conversion of PFAD (94.8%) with the lowest carbon deposition on the catalyst (approx. 5 wt.%). The selectivity of green fuel is gasoline 14.9%, jet fuel 45.0% and diesel 40.1%. For continuous operation, 12.5 wt.% Ni and 2.5 wt.% Cu were loaded on commercial pellet-type HZSM-5 catalyst. At operating temperature of 400?C, H2 pressure of 40 bar and H2/oil molar ratio of 100, the most suitable LHSV of 2 h-1 condition was found to achieve high conversion (90.1%) with a stable operation. The conversion gradually dropped over 80 h of the operating time (approx. 65.0% conversion) and then suddenly drops to 36.5% conversion at 103 h.