A Multi-Region Magnetoimpedance-Based Bio-Analytical System for Ultrasensitive Simultaneous Determination of Cardiac Biomarkers Myoglobin and C-Reactive Protein

Abstract

Cardiac biomarkers (CBs) are substances that appear in the blood when the heart is damaged or stressed. Measurements of the level of CBs can be used in course of diagnostics or monitoring the state of the health of group risk persons. A multi-region bio-analytical system (MRBAS) based on magnetoimpedance (MI) changes was proposed for ultrasensitive simultaneous detection of CBs myoglobin (Mb) and C-reactive protein (CRP). The microfluidic device was designed and developed using standard microfabrication techniques for their usage in different regions, which were pre-modified with specific antibody for specified detection. Mb and CRP antigens labels attached to commercial Dynabeads with selected concentrations were trapped in different detection regions. The MI response of the triple sensitive element was carefully evaluated in initial state and in the presence of biomarkers. The results showed that the MI-based bio-sensing system had high selectivity and sensitivity for detection of CBs. Compared with the control region, ultrasensitive detections of CRP and Mb were accomplished with the detection limits of 1.0 pg/mL and 0.1 pg/mL, respectively. The linear detection range contained low concentration detection area and high concentration detection area, which were 1 pg/mL–10 ng/mL, 10–100 ng/mL for CRP, and 0.1 pg/mL–1 ng/mL, 1 n/mL–80 ng/mL for Mb. The measurement technique presented here provides a new methodology for multi-target biomolecules rapid testing.

Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy

“…As presented in Table 1, CRP detection performance of our strategy was comparable to the reported in most of the other studies. [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]…”

“…2 The most widely used CRP diagnostic methods are antibody-based assays. 2 At present, several techniques including the use of a capacitive biosensor, 3 microuidic biosensor, 4-6 electrochemical biosensor, [7][8][9] sandwich immunoassay, 10,11 uorescent biosensor, 12 chemiluminescence immunoassay, 13 micro-uxgate sensor, 14 ber optic biosensor, 15 magnetoimpedancebased biosensor, 16 nanowire-mesh biosensor 17 and electrochemical impedance spectroscopy 18 have been developed for the detection of CRP. However, these methods for CRP detection are usually time-consuming, labor-intensive, complex in operation or preparation and involve expensive reagents.…”

Evaluation of analytical quality of cardiac biomarkers in the emergency laboratory by sigma metrics

“…The analytical performance of the tests in Group 1 was poor whereas the tests in Group 4 had world-class analytical quality. [6,7,8]. For diagnosis of AMI in the emergency units, myoglobin is a better marker rather than CKMB mass or cTnT within 3-6 hours after inception of symptoms, while CKMB mass is better at 7th hours.…”

Observing Dynamical Systems Using Magneto-Controlled Diffraction

“…[14]. The study of these agglomerates can be done in Ferrolens type devices to observe their evolution of biocompatible materials in the presence of magnetic fields, simulating the effects of these materials in conditions like those found in living tissues, where the flow is restricted due to the size of the vessels through which fluids flow, such as blood [7,8].…”

“…The potential applications for this kind of device is remarkable, for example the observation of light patterns in ferrofluids motivated some authors: to compare them with some atmospheric optics phenomena [2]; or to understand the cause behind the existence of light patterns [3], known as magnetic contours; or considering the ferrofluids as "smart nanofluid" [4], we can find some interesting studies of the optical properties controlled by an external magnetic field, such as the formation of ring structures of light in scattered patterns [5]; or in some exciting applications such as liquid robotics [6]. In addition to these cases, we have applications of nanoparticles in the medical field, because nanomaterials can offer a way to treat cancer without causing much damage to healthy tissue [7,8].…”