A multi‐residue screening method for the determination of 73 pesticides and metabolites in fruit and vegetables using high‐performance liquid chromatography/tandem mass spectrometry

Hetherton, Christel L.; Sykes, Mark; Fussell, Richard J.; Goodall, David M.

doi:10.1002/rcm.1645

Cited by 70 publications

(43 citation statements)

References 8 publications

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“…Identical recovery of isoproturon (84% and 85%, respectively) was achieved at both concentrations levels with good repeatability (7% and 5%, respectively). Achieved LOD (0.01 mg/kg) and LOQ (0.03 mg/kg) of isoproturon were comparable to other published methods for isoproturon determination in major crops (Herrera et al 1998;Hetherton et al 2004;Hernández et al 2006). Calibration curve, for which construction data were measured in 6 consecutive calibration sets (within the same analytical sequence) of spiked poppy seeds extracts, shows an excellent linearity (correlation coefficient, R 2 = 0.9984) over the whole calibration range (0.03-1 μg/ml).…”

Section: Optimisation and Methods Performance Characteristicssupporting

confidence: 75%

HPLC-MS/MS method for analysis of isoproturon in difficult matrix: poppy seeds

Kovalczuk¹,

Poustka²,

Hajšlová³

2008

Czech J. Food Sci.

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Kovalczuk T., Poustka J., Hajšlová J. (2008): HPLC-MS/MS method for analysis of isoproturon in difficult matrix: poppy seeds. Czech J. Food Sci., 26: 146-152.While several validated methods have been developed for analysis of phenylurea herbicides in staple food plants, analytical procedures suitable for their quantification in minor crops such as poppy seeds are not available. For the registration of isoproturon use in this crop, the documentation of dynamics of its residues following treatment was requested. To accomplish this task, HPLC-MS/MS method was developed. Extraction of residues was realised by methanol-water mixture, Supelclean LC-18 SPE cartridges were used for purification of crude extracts. For HPLC separation of isoproturon SPE fraction Lichrospher C18 column (25 cm × 4 mm, 5 µm) was employed. For detection and quantification of target analyte, mass spectrometer with ion trap analyser operated in positive atmospheric pressure chemical ionisation (APCI) and MS/MS mode was used. Following performance parameters of method were obtained: detection limit 0.01 mg/kg, recovery 84%, and repeatability 7%.

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Section: Optimisation and Methods Performance Characteristicssupporting

confidence: 75%

HPLC-MS/MS method for analysis of isoproturon in difficult matrix: poppy seeds

Kovalczuk¹,

Poustka²,

Hajšlová³

2008

Czech J. Food Sci.

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“…The number of active ingredients intended for the control of undesirable pests and weeds currently exceeds 800 (Tomlin 2002; Hertherton et al 2004;Fernández-Alba 2005). Avoiding the occurrence of some pesticide residues in the food supply is obviously impossible to achieve and, therefore, unsurprisingly, the health risk associated with a dietary intake of these chemicals has become a safety issue for both toxicologists and consumers.…”

Section: Introductionmentioning

confidence: 99%

“…Historically, gas chromatography (GC) used to be the main technique employed for this purpose. As documented in recent reviews (Hertherton et al 2004;Hernández et al 2006), GC-based multi-residue methods are still widely used in control laboratories worldwide; nevertheless, it should be noted that their scope becomes increasingly more insufficient. Many registered pesticides are relatively polar compounds not amenable to direct GC analysis, and on this account the development/validation of broad-scope procedures employing high-performance liquid chromatography (HPLC) for sample separation and selective detection strategy, such as tandem mass spectrometry (MS/MS), has become an urgent task.…”

Section: Introductionmentioning

confidence: 99%

Novel approach to fast determination of multiple pesticide residues using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)

Kovalczuk

Lacina

Jech

et al. 2008

Food Additives & Contaminants: Part A

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A rapid, high-throughput method employing ultra-performance liquid chromatography with tandem quadrupole mass spectrometry (UPLC-MS/MS) was developed and optimized for simultaneous quantification and confirmation of 64 pesticide residues and their toxic metabolites in fruit extracts prepared by a buffered QuEChERS procedure. The total time required for UPLC-MS/MS analysis was 8 min plus 2 min for re-equilibration to the initial UPLC conditions. Performance characteristics were determined for apple extracts spiked at 10 microg kg(-1). The repeatability of measurements expressed as relative standard deviations was in the range 1.5-13% at this level for most analytes. Thanks to very low limits of quantification (<10 microg kg(-1)for the majority of pesticides), an optimized method allows for the reliable control of not only common maximum residue limits (MRLs) set by European Union regulation for various pesticides/fruit combinations, but also of a uniform MRL of 10 microg kg(-1)endorsed for baby food.

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“…Both precursor and fragment ions observed using this technique were consistent with other methods reported in the literature for terbufos and terbufos sulfoxide. 7,16 The total DESI-MS analysis time required to confirm the presence of terbufos and diagnose organophosphate poisoning in the present case was approximately 5 min; whereas, the use of other traditional analytical techniques would have required several hours of sample preparation and analysis time before reaching a diagnosis. For example, gas chromatographymass spectrometry (GC-MS) was also used to confirm the presence of terbufos in the stomach contents sample from this case.…”

Section: Research-article2014mentioning

confidence: 84%

Rapid detection of terbufos in stomach contents using desorption electrospray ionization mass spectrometry

et al. 2014

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Abstract. Desorption electrospray ionization mass spectrometry (DESI-MS) is an emerging analytical technique that permits the rapid and direct analysis of biological or environmental samples under ambient conditions. Highlighting the versatility of this technique, DESI-MS has been used for the rapid detection of illicit drugs, chemical warfare agents, agricultural chemicals, and pharmaceuticals from a variety of sample matrices. In diagnostic veterinary toxicology, analyzing samples using traditional analytical instrumentation typically includes extensive sample extraction procedures, which can be time consuming and labor intensive. Therefore, efforts to expedite sample analyses are a constant goal for diagnostic toxicology laboratories. In the current report, DESI-MS was used to directly analyze stomach contents from a dog exposed to the organophosphate insecticide terbufos. The total DESI-MS analysis time required to confirm the presence of terbufos and diagnose organophosphate poisoning in this case was approximately 5 min. This highlights the potential of this analytical technique in the field of veterinary toxicology for the rapid diagnosis and detection of toxicants in biological samples.

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A multi‐residue screening method for the determination of 73 pesticides and metabolites in fruit and vegetables using high‐performance liquid chromatography/tandem mass spectrometry

Cited by 70 publications

References 8 publications

HPLC-MS/MS method for analysis of isoproturon in difficult matrix: poppy seeds

HPLC-MS/MS method for analysis of isoproturon in difficult matrix: poppy seeds

Novel approach to fast determination of multiple pesticide residues using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)

Rapid detection of terbufos in stomach contents using desorption electrospray ionization mass spectrometry

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