A multi-step nitrifying microbial enrichment to remove ammonia and nitrite in brackish aquaculture systems

Tuyet, Dao Thi Anh; Hiep, Le Minh; Binh, Ha Thi; Huyen, Le Thanh; Tang, Sen‐Lin; Chiang, Pei‐Wen; Hao, Do Manh

doi:10.1007/s10532-022-09988-9

Cited by 3 publications

(4 citation statements)

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“…Moreover, three kinds of low molecular and commonly used organic carbon sources, including sodium acetate, sodium citrate, and sucrose, were added into the enrichment medium for the enhancement of the enrichment process [27,38]. Good results were achieved in the enrichment period and biomass production compared to previous similar studies [16,24,25,37]. The enrichment process of nitrifying bioflocs was clearly shown by the concentration changes of NH 4 + -N, NO 2 − -N, NO 3 − -N, TIN, and input TN in the reactors with different organic carbon additions (Figure 1).…”

Section: Enrichment Process Of Nitrifying Bioflocs and The Effects Of...mentioning

confidence: 92%

“…In this study, a mixotrophic medium was designed and used, which was modified from the combination of autotrophic medium and artificial feed. Autotrophic medium had both NH 4 + -N and NO 2 − -N substrates for the concomitant growth of ammoniaand nitrite-oxidizing bacteria [36,37]. Artificial feed provided organic nitrogen and carbon sources for bacterial growth and, meanwhile, simulated the feeding environment of aquaculture systems.…”

Section: Enrichment Process Of Nitrifying Bioflocs and The Effects Of...mentioning

confidence: 99%

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Impact of Organic Carbons Addition on the Enrichment Culture of Nitrifying Biofloc from Aquaculture Water: Process, Efficiency, and Microbial Community

Wu,

Xu,

et al. 2024

Microorganisms

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In this study, we developed a rapid and effective method for enriching the culture of nitrifying bioflocs (NBF) from aquacultural brackish water. The self-designed mixotrophic mediums with a single or mixed addition of sodium acetate, sodium citrate, and sucrose were used to investigate the enrichment process and nitrification efficiency of NBF in small-scale reactors. The results showed that NBF with an MLVSSs from 1170.4 mg L−1 to 2588.0 mg L−1 were successfully enriched in a period of less than 16 days. The citrate group performed the fastest enrichment time of 10 days, while the sucrose group had the highest biomass of 2588.0 ± 384.7 mg L−1. In situ testing showed that the highest nitrification efficiency was achieved in the citrate group, with an ammonia oxidation rate of 1.45 ± 0.34 mg N L−1 h−1, a net nitrification rate of 2.02 ± 0.20 mg N L−1 h−1, and a specific nitrification rate of 0.72 ± 0.14 mg N g−1 h−1. Metagenomic sequencing revealed that Nitrosomonas (0.0~1.0%) and Nitrobacter (10.1~26.5%) were dominant genera for AOB and NOB, respectively, both of which had the highest relative abundances in the citrate group. Linear regression analysis further demonstrated significantly positive linear relations between nitrification efficiencies and nitrifying bacterial genera and gene abundance in NBF. The results of this study provide an efficient enrichment culture method of NBF for the operation of biofloc technology aquaculture systems, which will further promote its wide application in modern intensive aquaculture.

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Section: Enrichment Process Of Nitrifying Bioflocs and The Effects Of...mentioning

confidence: 92%

Section: Enrichment Process Of Nitrifying Bioflocs and The Effects Of...mentioning

confidence: 99%

Impact of Organic Carbons Addition on the Enrichment Culture of Nitrifying Biofloc from Aquaculture Water: Process, Efficiency, and Microbial Community

Wu,

Xu,

et al. 2024

Microorganisms

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“…N by the nitrifying bacteria in this water remediation experiment(Wang et al, 2020; Alarcón-Vivero et al, 2022;Tuyet et al, 2022). In the AA, the NO 2 -N concentrations were decreased rapidly and e ciently for the oxidative capacities of the free oxygen (DO) in the waters to nitrify the N(+ III) into N(+ V) under the conditions of the high concentration of DO (5.0 ± 0.6 mg/L) to increase the redox potential (ORP) about + 213 ± 37 mV, where the nal concentrations were all much lower than the ones of the anticipation.…”

mentioning

confidence: 83%

“…In the AA, the NO 2 -N concentrations were decreased rapidly and e ciently for the oxidative capacities of the free oxygen (DO) in the waters to nitrify the N(+ III) into N(+ V) under the conditions of the high concentration of DO (5.0 ± 0.6 mg/L) to increase the redox potential (ORP) about + 213 ± 37 mV, where the nal concentrations were all much lower than the ones of the anticipation. Although the DO concentrations were about maintained at 4.5 ± 0.6 mg/L in the IGR and 4.1 ± 0.3 mg/L in the SA which were much higher than the desired value of 2.0 mg/L of the nitri cation reaction demand, the quantities of the NO 2 -N amounts were oxidated by DO (O 2 ) to form the c compounds in the AA aeras, the left ones might not be well combined with O-atom in the SA and the IGR because of the low concentrations to delay and balance the nitri cation reaction with the lower chances to the probably collides until their coalescences(Qian et al, 2022;Tuyet et al, 2022). It was the reason that the decrease e ciencies of the NO 2 -N contaminants got less re ecting that the NO 2 -N contaminants respectively were of 0.14 ± 0.01 mg/L in the IGR and 0.11 ± 0.01 mg/L in the SA of Fig.3A, 0.23 ± 0.03 mg/L in the IGR and 0.16 ± 0.02 mg/L in the SA of Fig.3Band 0.23 ± 0.02 mg/L in the IGR and 0.16 ± 0.01 mg/L in the SA of Fig.3C.…”

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confidence: 93%

Fates of Nitrogenous Contaminants of Eutrophic Waters Using Laboratory Microcosm Method in Interception-Activation-Combined Bioreactors

Zhu

et al. 2022

Preprint

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For understanding the internal transformations of the nitrogen in nitrogenous contaminants like ammonia nitrogen (NH3-N), nitrite nitrogen (NO2-N) and nitrate nitrogen (NO3-N) in natural waters, the remediation effects of the designed reactors on NH3-N, NO2-N and NO3-N were well carried out, respectively. It was not like the NO3-N contaminants which would be rapidly whittled down within any concentrations of this experiment, meanwhile the concentrations of the NH3-N and NO3-N contaminants were respectively reduced by nitrification and denitrification from 5.00 mg/L and 10.00 mg/L in influents under the conditions of dissolved oxygen concentrations of 5.0 ± 0.6 mg/L and the redox potential (ORP) about + 213 ± 37 mV at 20.0 ± 0.5℃ of water temperature to no more than 2.0 mg/L in effluents. Both NH3-N and NO3-N concentrations were under the limited concentrations of the level V of GB 3838 − 2002 with the help of the aerobic granular sludges (AGS) cultured and collected from the interception-reaction galleries (IRG) of the reactors of characteristics of 0.20–2.00 mm in statistical particle diameter (93 ± 4% m/m), ash contents of 7 ± 5% m/m and the degradation capacities of 0.88 ± 0.13 (mg nitrogen)/(mg sludge). Thereafter, it was not decreased as expected to the finial acceptable concentration of the prospective value of 2.00 mg/L with the original concentration of 15.00 mg/L in influents. Thus, the processes of the nitrogenous contaminants could be remediated within the concentrations of the rage of no more than 10.00 mg/L, especially both NH3-N and NO3-N to friendlily purify the natural waters without any other exogenous powers and more energy consumptions to the environment.

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Exogenous compound bacteria enhance the nutrient removal efficiency of integrated bioremediation systems: Functional genes and microorganisms play key roles

Wei,

Shen,

Nicholaus

et al. 2024

Environmental Research

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A multi-step nitrifying microbial enrichment to remove ammonia and nitrite in brackish aquaculture systems

Cited by 3 publications

References 32 publications

Impact of Organic Carbons Addition on the Enrichment Culture of Nitrifying Biofloc from Aquaculture Water: Process, Efficiency, and Microbial Community

Impact of Organic Carbons Addition on the Enrichment Culture of Nitrifying Biofloc from Aquaculture Water: Process, Efficiency, and Microbial Community

Fates of Nitrogenous Contaminants of Eutrophic Waters Using Laboratory Microcosm Method in Interception-Activation-Combined Bioreactors

Exogenous compound bacteria enhance the nutrient removal efficiency of integrated bioremediation systems: Functional genes and microorganisms play key roles

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