“…Various reconstruction methods have been developed to date and significant progress has been achieved [5,[7][8][9][10][11][12][13][14]. However, there is no error estimation method that evaluates the corresponding reconstruction results associated with unclear nonlinearities in the spikecalcium relationship [9,15,16], contamination of signals from other cellular parts [4], system noise [17], and the often relatively low temporal resolution of the calcium signal recording compared to the electrical signal [14], along with the lack of a strict mathematical model describing the relationship between the Ca 2+ trace and spike firing (especially burst firing) [18,19].…”