A Multicelled Sample Container for the Rapid Counting of P32 in Blood

Lawson, Hampden; Coleman, Everett S.; Adams, Samuel B.

doi:10.1063/1.1771082

Cited by 1 publication

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“…Mongrel dogs were anaesthetized with sodium barbital, 250 mg/kg. Plasma and cell volumes were measured simultaneously and independently by means of human serum albumin labelled with 131I and erythrocytes tagged with 32p, utilizing techniques described previously (Chacalos & Moore, 1963;Lawson, Coleman & Adams, 1954). In essence the 142 VARIATIONS IN VESSEL VOLUMES 143 techniques involved the injection of a 5 ml.…”

Section: Methodsmentioning

confidence: 99%

“…Plasma and cell volumes were measured simultaneously and independently by means of human serum albumin labelled with 131I and erythrocytes tagged with 32p, utilizing techniques described previously (Chacalos & Moore, 1963;Lawson, Coleman & Adams, 1954 (Nickerson, Sear & Reeve, 1953;Berlin, Huff, Van Dyke & Hennesy, 1949;Mollison & Veall, 1955). Any loss of either isotope from the circulating blood tends to be corrected by the multiple sampling technique and the slopes of the extrapolation curves.…”

Section: Methodsmentioning

confidence: 99%

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Variations in small and large vessel volumes caused by cations, adrenaline and histamine*

Chacalos¹

1967

The Journal of Physiology

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SUMMARY1. Plasma and blood cell volumes were measured simultaneously with [1311]albumin and [32P]erythrocytes, together with the arterial haematocrits, in acutely splenectomized dogs anaesthetized with sodium barbital. These measurements were made during control runs and after the administration of vasoactive substances.2. With these data and previously derived equations the mean composite radius of all small vessels with a radius < 150 ,t, the distribution of the blood volumes between small and large vessels, and other variables could be calculated.3. Adrenaline, CaCl2 or vasopressin administration caused a reduction of the mean composite radius, a net shift of erythrocytes and plasma out of the small vessel volume, and an expansion of the large vessel volume. Histamine, KCI and MgC12 produced opposite effects.4. Adrenaline appeared to cause the release of sequestered erythrocytes into the active circulation.5. The effects of CaCl2 and KCI persisted for several hours, while those of MgCl2 did not.6. The effects of vasopressin could be reversed by histamine, those of adrenaline by splenic plasma or KCI, and those of CaCl2 by MgC12.7. It was suggested that the observed vasoactions are essentially the resultants of diverse combinations of direct and indirect actions on the capacitance section of the small vessel volume.

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Section: Methodsmentioning

confidence: 99%