A multicenter evaluation of the routine use of a new white cell‐ reduction apheresis system for collection of platelets

Abstract

The protocol selected in the initial phase, now available as a WBC-reduction system, results in platelet concentrates with very low residual WBC levels. This satisfies even the most stringent criteria for WBC reduction in platelets, without the platelet loss typically seen with conventional fiber filtration.

The effect of leukocyte-reduction method on the amount of human cytomegalovirus in blood products: a comparison of apheresis and filtration methods

“…Platelet apheresis was performed using the COBE Spectra LRS or LRS TURBO (Gambro BCT, Lakewood, CO). 11 Residual WBCs in leukocyte-reduced blood products were determined at Gambro BCT by a previously described flow cytometry protocol. 12 Products collected in Denver were shipped overnight with 22°C gel packs from Lakewood to Norfolk for CMV QA-PCR.…”

Liquid–Stored Red Blood Cells for Transfusion

“…Some of the unsatisfactory results of leukodepletion that have been reported even during this decade, may no longer be valid [9, 10], although quality assurance of individual filters remains an issue. Some apheresis procedures such as the Spectra (Cobe BCT, Lakewood, Colo., USA) and Amicus (Baxter Healthcare, Deerfield, Ill., USA), can produce platelet concentrates of sufficient purity to make subsequent filtration unnecessary [11, 12]. …”

A Prospective Crossover Trial Comparing Performance and in vitro Platelet Quality of Three New Apheresis Devices with Current Equipment

“…Even in the absence of severe side effects during automated collection procedures [6], the latter has been of growing interest as relatively high rates of citrate reactions have been described recently with new devices [7]. Apart from efficiency and donor comfort, apheresis systems should guarantee consistent product quality, particularly in terms of reliable attainment of the targeted PLT dose and consistent leukoreduction, performed either by filtration or by physical separation, utilizing different cell densities of PLTs and mononuclear cells [8,9]. The white blood cell (WBC) purity obtained by the latter techniques is reported to be more consistent than that obtained upon filtration [10,11].…”