A multichannel microcalorimetric system

Suurkuusk, J.; Suurkuusk, Malin; Vikegard, Peter

doi:10.1007/s10973-017-6684-7

Cited by 17 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As expected, the strong tendency of A bases to stack is on display through the Na + titration. The CD experiments and SAXS-derived modelling show that the initial low salt (Na + only) starting chain state features a high number of stacked bases, in good agreement with both MD predictions and experimental observations 22,32,37,39,71 . These stacked geometries typically provide resistance against both large scale structural changes and ion binding interactions, as the winding phosphate backbone fails to provide enough coordination points to capture and dehydrate ions in the atmosphere 72 .…”

Section: Discussionsupporting

confidence: 79%

“…We selected chains of 30 U nucleotides, rU 30 , and contrast their conformations with those of rA 30 , containing A nucleotides. Past work suggests these sequences have disparate characteristics and display a wide variation of arrangements 22,28,[37][38][39][40][41][42] . Additionally, the presence of these particular sequences within functionally distinct RNAs, e.g.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Visualizing Disordered Single-Stranded RNA: Connecting Sequence, Structure, and Electrostatics

2019

View full text Add to dashboard Cite

Disordered homopolymeric regions of single-stranded RNA, such as U or A tracts, are found within functional RNAs where they play distinct roles in defining molecular structure and facilitating recognition by partners. Despite this prominence, details of conformational and biophysical properties of these regions have not yet been resolved. We apply a number of experimental techniques to investigate the conformations of these biologically important motifs, and provide quantitative measurements of their ion atmospheres. Single strands of RNA display pronounced sequence dependent conformations that relate to the unique ion atmospheres each attracts. Chains of rU bases are relatively unstructured under all conditions, while chains of rA bases display distinct ordering, through stacking or clustering motifs, depending on the composition of the surrounding solution. These dramatic structural differences are consistent with the measured disparity in ion composition and atmospheres around each homopolymer, revealing a complex interplay of base, ion and single-strand ordering. The unique structural and ionic signatures of homopolymer ssRNAs explains their role(s) in folding structured RNAs, and may explain their distinct recognition by protein partners.

show abstract

Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Visualizing Disordered Single-Stranded RNA: Connecting Sequence, Structure, and Electrostatics

2019

View full text Add to dashboard Cite

show abstract

“…An average value of 1.35 J g −1 K −1 for both W1 and W2, obtained from four different tests, was in good agreement with the assumed value. The W1 and W2 samples were used as received from the waste storage facility regarding the moisture content, which was estimated to be approximately 30% on dry mass basis, as obtained after heating the sample in an oven at 105 °C for 24 h [18].…”

Section: Sample Preparationmentioning

confidence: 99%

Heat production in municipal and industrial waste as revealed by isothermal microcalorimetry

Pushp

Lönnermark

Hedenqvist

et al. 2021

J Therm Anal Calorim

Self Cite

View full text Add to dashboard Cite

Self-ignited fires at municipal solid waste (MSW) storage sites are relatively common. The minimization of the phenomenon of self-heating in the waste can reduce the risks for smouldering combustion. The purpose of this work was to develop a method that can be used to measure and characterize the heat production in MSW. The method is based on isothermal heat conduction microcalorimetry (IMC). The heat production in MSW was determined based on sampling from two sites in two different geographical locations in Sweden. Both the original waste and milled/homogenised waste were tested. The heat production was measured at different temperatures together with gas analysis using micro-gas chromatography. The activity in the waste, in terms of its heat flow, increased when the temperature increased up to 60 °C and decreased at higher temperatures, e.g., 70 and 80 °C. The consumption of oxygen and the production of carbon dioxide, together with the heat production, indicated that aerobic metabolism was responsible for the heat production. This is further strengthened by the marginal heat production observed for ultraviolet treated waste. The results showed that IMC is a valuable tool for characterising the self-heating in municipal and industrial waste.

show abstract

“…Along the last century, soil began to be considered as a source of energy, too, and the soil microbial metabolism (SMM) began to be monitored on heat basis [10,11]. The development of highly sensitive isothermal calorimeters makes it possible to quantify the heat released by SMM [12] and to consider soil as a thermodynamic system where SOM is the reservoir of energy fueling the soil microbial community. Therefore, we have the two main ingredients to develop soil thermodynamics: mass and energy.…”

Section: Introductionmentioning

confidence: 99%

Thermodynamics of Soil Microbial Metabolism: Applications and Functions

Barros

2021

Applied Sciences

View full text Add to dashboard Cite

The thermodynamic characterization of soils would help to study and to understand their strategies for survival, as well as defining their evolutionary state. It is still a challenging goal due to difficulties in calculating the thermodynamic state variables (enthalpy, Gibbs energy, and entropy) of the reactions taking place in, and by, soils. Advances in instrumentation and methodologies are bringing options for those calculations, boosting the interest in this subject. The thermodynamic state variables involve considering the soil microbial functions as key channels controlling the interchange of matter and energy between soil and the environment, through the concept of microbial energy use efficiency. The role of microbial diversity using the energy from the soil organic substrates, and, therefore, the who, where, with whom, and why of managing that energy is still unexplored. It could be achieved by unraveling the nature of the soil organic substrates and by monitoring the energy released by the soil microbial metabolism when decomposing and assimilating those substrates. This review shows the state of the art of these concepts and the future impact of thermodynamics on soil science and on soil ecology.

show abstract

A multichannel microcalorimetric system

Cited by 17 publications

References 15 publications

Visualizing Disordered Single-Stranded RNA: Connecting Sequence, Structure, and Electrostatics

Visualizing Disordered Single-Stranded RNA: Connecting Sequence, Structure, and Electrostatics

Heat production in municipal and industrial waste as revealed by isothermal microcalorimetry

Thermodynamics of Soil Microbial Metabolism: Applications and Functions

Contact Info

Product

Resources

About