A multicolor biosensor for alkaline phosphatase activity detection based on the peroxidase activity of copper nanoclusters and etching of gold nanorods

Luo, Qin; Lin, Yow-Jian; Cai, Qipeng; Luo, Fang; Lin, Cuiying; Wang, Jian; Qiu, Bin; Lin, Zhenyu

doi:10.1039/d2an00517d

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…The target can keep the shape of the LSPR unchanged by hindering the etching of Au NPs and the etching rate can be varied by controlling the concentration of the inhibitor under specific conditions. Luo et al developed a multicolor biosensor for ALP activity detection based on the peroxidase-like activity of Cu NCs and the etching of Au NRs [ 119 ]. Cu NCs could catalyze the oxidation of TMB to generate TMB 2+ , which in turn etched Au NRs, and the etching process was accompanied by a significant change in the color of the system.…”

Section: Sensing Strategy Based On the Lspr Properties Of Au Npsmentioning

confidence: 99%

Chromogenic Mechanisms of Colorimetric Sensors Based on Gold Nanoparticles

Cui

Zhao

2023

Biosensors

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The colorimetric signal readout method is widely used in visualized analyses for its advantages, including visualization of test results, simple and fast operations, low detection cost and fast response time. Gold nanoparticles (Au NPs), which not only exhibit enzyme-like activity but also have the advantages of tunable localized surface plasmon resonance (LSPR), high stability, good biocompatibility and easily modified properties, provide excellent platforms for the construction of colorimetric sensors. They are widely used in environmental monitoring, biomedicine, the food industry and other fields. This review focuses on the chromogenic mechanisms of colorimetric sensors based on Au NPs adopting two different sensing strategies and summarizes significant advances in Au NP-based colorimetric sensing with enzyme-like activity and tunable LSPR characteristics. In addition, the sensing strategies based on the LSPR properties of Au NPs are classified into four modulation methods: aggregation, surface modification, deposition and etching, and the current status of visual detection of various analytes is discussed. Finally, the review further discusses the limitations of current Au NP-based detection strategies and the promising prospects of Au NPs as colorimetric sensors, guiding the design of novel colorimetric sensors.

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Section: Sensing Strategy Based On the Lspr Properties Of Au Npsmentioning

confidence: 99%

Chromogenic Mechanisms of Colorimetric Sensors Based on Gold Nanoparticles

Cui

Zhao

2023

Biosensors

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“…In recent years, the application and research of noble metal nanomaterials have opened a new development path for colorimetric biosensors. − It is well known that gold nanorods (Au NRs) are a good chromogenic agent for visualization sensors and have been applied to design many visualization sensors with excellent performance. , For example, Ma et al revealed that the ultimate yellow product (TMB 2+ ) of the horseradish peroxidase (HRP)-TMB catalytic system is able to quantitatively etch Au NRs, resulting in a significant color change. In this study, once the immunocomplex is formed, the HRP bound to the ELISA plate oxidizes TMB into TMB + , and TMB + is oxidized to TMB 2+ under acidic conditions.…”

Section: Introductionmentioning

confidence: 99%

Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids

et al. 2023

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The detection of trypsin is significantly important for both clinical diagnosis and disease treatment. In this study, an innovative multicolor sensor for trypsin detection has been established based on the regulation of the peroxidase activity of bovine serum albumin-coated gold nanoclusters (BSA−Au NCs) and efficient etching of gold nanobipyramids (Au NBPs). BSA−Au NCs have slight peroxidase enzyme activity and can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate TMB + , while trypsin can hydrolyze BSA ligands on the surface of BSA−Au NCs, thus exposing more catalytic active sites of BSA−Au NCs and resulting in the enhancement of the peroxidase activity of BSA−Au NCs, hence more TMB + is generated. Under acidic conditions, TMB + can etch Au NBPs efficiently, consequently affecting the aspect ratio of Au NBPs accompanied by the ultraviolet−visible (UV−vis) spectra blue shifting of the system. Furthermore, this also results in color variations that can be distinguished and recognized by naked eyes without any expensive and sophisticated instruments. This multicolor sensor has an available linear relationship with the logarithm of the trypsin concentration in the range of 0.1−100 μg/mL, and the detection limit is 0.045 μg/mL. The designed sensor has been used to detect the concentration of trypsin in human serum samples from healthy individuals and pancreatitis patients with satisfactory results.

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Hairpin probe-based one-pot multiplex isothermal amplification combined with bifunctional G-quadruplex (IHP-GT) for the detection of alkaline phosphatase

Zhu,

Zhou,

Deng

et al. 2024

Analytica Chimica Acta

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A multicolor biosensor for alkaline phosphatase activity detection based on the peroxidase activity of copper nanoclusters and etching of gold nanorods

Abstract: A multicolor biosensor for ALP activity has been developed based on the peroxidase activity of copper nanoclusters and etching of gold nanorods.

Cited by 4 publications

References 33 publications

Chromogenic Mechanisms of Colorimetric Sensors Based on Gold Nanoparticles

Chromogenic Mechanisms of Colorimetric Sensors Based on Gold Nanoparticles

Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids

Hairpin probe-based one-pot multiplex isothermal amplification combined with bifunctional G-quadruplex (IHP-GT) for the detection of alkaline phosphatase

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