A multicommutated tester of bioreactors for flow analysis

Pokrzywnicka, Marta; Kaminski, Jacek K; Michalec, Michał; Koncki, Robert; Tymecki, Łukasz

doi:10.1016/j.talanta.2016.07.008

Cited by 12 publications

(3 citation statements)

References 48 publications

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“…After solidification, a cylindrical fluidic chamber with inner diameter 8 mm was punched with the biopsy punch. The screen-printed three-electrode system comprised a 4 mm-diameter working electrode (DropSens, Llanera, Spain), which was used after amination [23]. For amination, the electrode was dipped in a 0.5 M ammonium carbamate solution, and cyclic voltammetry (CV) was performed.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, urea measurement in the flow condition has a clear and irreplaceable advantage, real-time monitoring. The use of a bioreactor for flow analysis was reported recently [23]. However, this bioreactor required a long reaction time and measured only a single light signal from a single injection of urea after a complex transferring process.…”

Section: Introductionmentioning

confidence: 99%

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Parylene-Coated Polytetrafluoroethylene-Membrane-Based Portable Urea Sensor for Real-Time Monitoring of Urea in Peritoneal Dialysate

Park

Kim

et al. 2019

Sensors

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A portable urea sensor for use in fast flow conditions was fabricated using porous polytetrafluoroethylene (PTFE) membranes coated with amine-functionalized parylene, parylene-A, by vapor deposition. The urea-hydrolyzing enzyme urease was immobilized on the parylene-A-coated PTFE membranes using glutaraldehyde. The urease-immobilized membranes were assembled in a polydimethylsiloxane (PDMS) fluidic chamber, and a screen-printed carbon three-electrode system was used for electrochemical measurements. The success of urease immobilization was confirmed using scanning electron microscopy, and fourier-transform infrared spectroscopy. The optimum concentration of urease for immobilization on the parylene-A-coated PTFE membranes was determined to be 48 mg/mL, and the optimum number of membranes in the PDMS chamber was found to be eight. Using these optimized conditions, we fabricated the urea biosensor and monitored urea samples under various flow rates ranging from 0.5 to 10 mL/min in the flow condition using chronoamperometry. To test the applicability of the sensor for physiological samples, we used it for monitoring urea concentration in the waste peritoneal dialysate of a patient with chronic renal failure, at a flow rate of 0.5 mL/min. This developed urea biosensor is considered applicable for (portable) applications, such as artificial kidney systems and portable dialysis systems.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Parylene-Coated Polytetrafluoroethylene-Membrane-Based Portable Urea Sensor for Real-Time Monitoring of Urea in Peritoneal Dialysate

Park

Kim

et al. 2019

Sensors

View full text Add to dashboard Cite

show abstract

“…Thus, urea measurement in the flow condition has clear and irreplaceable advantage, real-time monitoring. The use of a bioreactor for flow analysis was reported recently [24]. However, this bioreactor required a long reaction time and measured only a single light signal from a single injection of urea after a complex transferring process.…”

Section: Introductionmentioning

confidence: 99%

Parylene-Coated PTFE-Membrane-Based Portable Urea Sensor for Use in Fast Flow Conditions

Park¹,

Kim²,

Kim³

et al. 2019

Preprint

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A portable urea sensor for use in the fast flow condition was fabricated using porous polytetrafluoroethylene (PTFE) membranes coated with amine-functionalized parylene, parylene-A, by vapor deposition. To generate a specific electrochemical sensor signal from urea, the urea-hydrolyzing enzyme urease was immobilized on the parylene-A-coated PTFE membranes via chemical crosslinking using glutaraldehyde. The urease-immobilized membranes were assembled in a polydimethylsiloxane (PDMS) fluidic chamber, and a screen-printed carbon three-electrode system was used for electrochemical measurements. The success of urease immobilization was confirmed using fluorescence microscopy, scanning electron microscopy, and Fourier-transform infrared spectroscopy. The optimum concentration of urease for immobilization on the parylene-A-coated PTFE membranes was determined to be 48 mg/mL, and the optimum number of membranes in the PDMS chamber was found to be 8. Using these optimized conditions, we fabricated the urea biosensor and monitored urea samples under various flow rates ranging from 0.5 to 10 mL/min in the flow condition using chronoamperometry. To test the applicability of the sensor for physiological samples, we used it for monitoring urea concentration in the waste peritoneal dialysate of a patient with chronic renal failure, at a flow rate of 0.5 mL/min.

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pH-modulated aggregation-induced emission of Au/Cu nanoclusters and its application to the determination of urea and dissolved ammonia

2021

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A multicommutated tester of bioreactors for flow analysis

Cited by 12 publications

References 48 publications

Parylene-Coated Polytetrafluoroethylene-Membrane-Based Portable Urea Sensor for Real-Time Monitoring of Urea in Peritoneal Dialysate

Parylene-Coated Polytetrafluoroethylene-Membrane-Based Portable Urea Sensor for Real-Time Monitoring of Urea in Peritoneal Dialysate

Parylene-Coated PTFE-Membrane-Based Portable Urea Sensor for Use in Fast Flow Conditions

pH-modulated aggregation-induced emission of Au/Cu nanoclusters and its application to the determination of urea and dissolved ammonia

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