A multidimensional overpressured layer chromatographic method for the characterization of tetrazine libraries

Gombosuren, Naran; Novàk, Zoltán; Kotschy, András; Mincsovics, Emil; Dibó, Gábor

doi:10.1016/j.jbbm.2005.12.005

Cited by 9 publications

(3 citation statements)

References 30 publications

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“…OPLC also offers an advantage over traditional TLC as it is a sealed system, which results in less solvent loss through evaporation [11–12]. As such, OPLC has been widely used in analytical and preparative applications [13–15]. Compared to conventional methods, OPLC offers a more rapid and reliable method for the isolation of naturally occurring compounds.…”

Section: Introductionmentioning

confidence: 99%

Antifungal Metabolites from the Roots of Diospyros virginiana by Overpressure Layer Chromatography

Wang

Habib

León

et al. 2011

Chemistry & Biodiversity

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A preparative overpressure layer chromatography (OPLC) method was successfully used for the separation of two new natural compounds, 4-hydroxy-5,6-dimethoxy-2-naphthaldehyde (1) and Δ12,13-20,29-dihydrobetulin (2) together with nine known compounds including 7-methyl-juglone (3), diospyrin (4), isodiospyrin (5), shinanolone (6), lupeol (7), betulin (8), betulinic acid (9), betulinaldehyde (10), and ursolic acid (11) from the acetone extract of the roots of Diospyros virginiana. Their identification was performed with mono and bidimensional NMR spectroscopy and HR-ESI-MS methods. All the isolated compounds were evaluated for their antifungal activity against Colletotrichum fragariae, C. gloeosporioides, C. acutatum, Botrytis cinerea, Fusarium oxysporum, Phomopsis obscurans, and P. viticola using in vitro micro-dilution broth assay. The results indicated that compounds 3 and 5 showed high antifungal activity against P. obscurans at 30 μM with 97.0 % and 81.4 % growth inhibition and moderate activity against P. viticola (54.3 % and 36.6 %). It appears that an optimized OPLC system offers a rapid and efficient method of exploiting bioactive natural products.

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Section: Introductionmentioning

confidence: 99%

Antifungal Metabolites from the Roots of Diospyros virginiana by Overpressure Layer Chromatography

Wang

Habib

León

et al. 2011

Chemistry & Biodiversity

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“…The spot capacity of fully off-line OPLC can be dramatically increased by means of two-dimensional separation. This has been used for the perfect separation of sixteen closely related coumarins from the genus Angelica [66] and also for the separation of 14 tetrazine derivatives prepared by parallel combinatorial synthesis [67]. The separation of dinitrophenylhydrazones of saturated aldehydes and ketones has been elaborated using normal and reversed phases and different eluents.…”

Section: Overpressured Layer Chromatography (Oplc)mentioning

confidence: 99%

Overpressured Layer Chromatography (OPLC) – A Flexible Tool of Analysis and Isolation

Mincsovics¹,

Tyihák

2011

Natural Product Communications

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This review briefly summarizes the overpressured layer chromatography (OPLC) technique and its progress from the beginning until today. Some theoretical aspects, important technical and methodological solutions, as well as analytical separations and isolations are demonstrated covering the last 30 years. The infusion and transfusion OPLC operations, as well as their combination, and their off-line and on-line processes, and off-line and on-line hyphenations for detection and structure elucidation are presented. The combination of OPLC separation with biological detection by direct bioautography and BioArena as an important solution touches the potential of analysis and isolation based on biological activity.

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“…OPLC was combined with UV densitometry and near-infrared (NIR) spectrometry directly on the layer for qualitative and quantitative pharmaceutical analysis . Other published applications of OPLC included a complete multidimensional separation of a tetrazine library prepared by parallel combinatorial synthesis obtained using 2D OPLC , biochemical examination of the general defense mechanism of plants , and separation and quantitative determination of seven aldoses and alditols in hemicellulose hydrolyzates [aluminum foil backed OPLC-HPTLC plates, ACN−acetic acid−water (63:33:5) mobile phase, overrunning elution] .…”

Section: Apparatus and Techniquesmentioning

confidence: 99%

Planar Chromatography

Sherma

2008

Anal. Chem.

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This review covers the literature of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) found by computer-assisted searching in Chemical Abstracts and the ISI Web of Science from November 1, 2005 to November 1, 2007. The literature search was augmented by consulting Analytical Abstracts, and the following journals that regularly publish papers on TLC were searched directly: Journal of Chromatography (parts A and B and the bibliography issues),

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A multidimensional overpressured layer chromatographic method for the characterization of tetrazine libraries

Cited by 9 publications

References 30 publications

Antifungal Metabolites from the Roots of Diospyros virginiana by Overpressure Layer Chromatography

Antifungal Metabolites from the Roots of Diospyros virginiana by Overpressure Layer Chromatography

Overpressured Layer Chromatography (OPLC) – A Flexible Tool of Analysis and Isolation

Planar Chromatography

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