A multifactorial assessment of the SRP pathway constituent FtsY as a vital mycobacterial constituent

Abstract

The signal recognition particle (SRP) system plays an imperative role in transporting the secretory protein to its intended location. The SRP pathway running in Mycobacterium tuberculosis constitutes FtsY (signal receptor), FfH (SRP), and 4.5S RNA in which signal receptor acts in the GTP-dependent manner. In this study, we are rendering the essential facts of FtsY with respect to mycobacterial growth. The growth study experiment showed that downexpressed FtsY slowed the growth of Mycobacterium smegmatis mc 2 155 from the initial lag phase to stationary phase. Previously, we have showed that GTPase activity of FtsY is metal ion dependent and showed the maximum activity with 10 mM magnesium. The effect of Mg 2+ and Mn 2+ on mycobacterial growth showed that Mg 2+ did not affect the growth, whereas higher concentration of Mn 2+ decreases the bacterial growth. After searching the inhibitor database, 14 GTPase and ATPase inhibitors,