A multifactorial system controlling myeloid cell differentiation and division

Blazsek, I; Balázs, András; Gaál, D.; Holczinger, L.

doi:10.1016/0047-6374(76)90008-7

Cited by 6 publications

(2 citation statements)

References 22 publications

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“…In contrast to earlier experiments [5,8], GCE was isolated from peripheral horse blood. The slightly heparinised blood (5000 U/l, Richter, Budapest) was left to sediment spontaneuously at 37 °C for 30 minutes, the leukocyte layer sucked off.…”

Section: Preparation Of the Extractsmentioning

confidence: 99%

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In Vitro Sensitivity of Transplantable Leukemias to Endogenous Granuloid (GCE, GI–2) and Lymphoid (T4, T4–1) Inhibitors of Proliferation

Balázs¹,

Gál²,

Klupp³

et al. 1978

Oncology

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The effect on cell proliferation of crude granulocyte and thymocyte extracts (GCE, T₄) and of their target-specific fractions (Gl-2, T₄-1) was studied in cultures with transplantable subacute myeloid and lymphoid leukemia (ML, LL). In the dose rage studied (1–500 μg/ml) each factor reduced ³H-TdR incorporation into acid-insoluble DNA of bone marrow, thymus and spleen cells with ML or LL as a function of the dose, approximately linearly. Normal bone marrow proved to be less sensitive to GCE than the ML one: according to parallel line bioassay by a factor of μ = 0.56. The reactivity of LL spleen and thymus is also higher to medium T₄-1 concentrations (50–200 μg/ml) than that of normal lymphoid populations. T₄-1 inhibits ³H-TdR incorporation into the DNA of LL spleen cells sub-maximally in 90’: this effect lasts for > 7 hours. Because of its more homogeneous cell composition and higher sensitivity, subacute myeloid leukemia is more suitable for screening endógenous granuloid inhibitors than are homologous normal cell cultures.

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Section: Preparation Of the Extractsmentioning

confidence: 99%

“…GI-2, Ta and Ta-l were added to the cultures on explantation . in graded concentrations of 1-1000 ug/ml: their protein contents were determined according to Hartree [8],…”

Section: Preparation Of the Extractsmentioning

confidence: 99%