A multifunctional lipoxygenase from Pyropia haitanensis— The cloned and functioned complex eukaryotic algae oxylipin pathway enzyme

Chen, Hai-min; Zhu, Zhujun; Chen, Juanjuan; Yang, Rui; Luo, Qijun; Xu, Jilin; He, Shan; Yan, Xiaojun

doi:10.1016/j.algal.2015.09.015

Cited by 24 publications

(47 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…13-HpOTE was found to be downregulated other than upregulated during our observation of oxypilins. Considering that 13-HpOTE is the precursor of JA synthesis, and PhLOX1 possesses allene oxide synthase capability (Chen et al 2015), our findings suggest P. haitanensis is capable of catabolizing 13-HpOTE to form JA. This indicated that there is positive correlation between the changes of 13-HpOTE and JA.…”

Section: Discussionmentioning

confidence: 78%

“…Several LOXs have been identified in P. haitanensis , among which two were reported (Zhu et al ; Chen et al ). Whether they can generate different combinations of oxylipins by regulating the expression and activity of LOXs to adapt to diverse environments remains enigmatic.…”

Section: Discussionmentioning

confidence: 99%

“…In previous report, we indicated that PhLOX increased HPL and rapidly degrade hydroperoxide intermediates into unsaturated aldehydes and alcohols. However, C18:2 can only be catalyzed into 9‐HpODE without further degradation (Chen et al ). PhLOX2 did not exhibit HPL function but formed a large amount of hydroperoxides and hydroxyl derivatives (Zhu et al ).…”

Section: Discussionmentioning

confidence: 99%

“…But PhHsp70, a gene plays a critical role in the development of thermotolerance and protection from cellular damage associated with stresses, has not upregulated neither, indicates that PhHsp70 may not involve in the response of P. haitanensis to flg22 (Kregel 2002). Several LOXs have been identified in P. haitanensis, among which two were reported Chen et al 2015). Whether they can generate different combinations of oxylipins by regulating the expression and activity of LOXs to adapt to diverse environments remains enigmatic.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Flg22‐triggered oxylipin production in Pyropia haitanensis

Yang

et al. 2017

Phycological Research

Self Cite

View full text Add to dashboard Cite

A variety of different symbiotic microbial communities are harbored on the surface of seaweeds, the interactions of which depend upon nutritional exchanges between the microbes and the hosts. Metabolomic profiling is able to provide a comprehensive and unbiased snapshot of the metabolites associated with seaweed-microbe interactions. In this study, the relationships between phycosphere bacteria and the red alga Pyropia haitanensis were investigated on a metabolomic basis using gas chromatography-mass spectrometry, and the pathways of the interactions between the seaweed and its associated phycospheric microbes were revealed. Bacillus sp. WPySW2, one bacterial species isolated from the phycosphere of Pyropia species, had a significant influence on the metabolomic profile of the algae. Some of the intracel-lular metabolites such as phenylalanine, leucine, isoleucine, valine, proline, tyrosine, threonine, octadecanoic acid, hexadecanoic acid, and citric acid were downregulated in the thalli of P. haitanensis when it was co-cultured with Bacillus sp. WPySW2, while several special metabolites including melibiose, serine, glycerol-3-phosphate, galactosylglycerol, and alanine were upregulated. The results demonstrated that P. haitanensis grew better when it was co-cultured with Bacillus sp. WPySW2 at 20 °C. In conclusion, several main intracellular metabolites were downregulated and upregulat-ed, which might have facilitated bacterial colonization.

show abstract

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Flg22‐triggered oxylipin production in Pyropia haitanensis

Yang

et al. 2017

Phycological Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…6,7) A brown alga, Laminaria angustata, and a red alga, Pyropia haitanensis, are also reported to have the ability to form C6 aldehydes. 8,9) Recent analysis on the genome sequence of a moss, Physcomitrella patens, indicated that the genome has two AOSs involved in oxo-phytodienoic acid synthesis and one HPL involved in C9 aldehyde synthesis. Two CYP74 genes found in the genome of Marchantia polymorpha encoded AOSs and showed no HPL activities to form C6 aldehydes from 13-hydroperoxide of C18 fatty acid.…”

mentioning

confidence: 99%

n-Hexanal and (Z)-3-hexenal are generated from arachidonic acid and linolenic acid by a lipoxygenase in Marchantia polymorpha L.

Tawfik

Yamato

Kohchi

et al. 2017

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Most terrestrial plants form green leaf volatiles (GLVs), which are mainly composed of six-carbon (C6) compounds. In our effort to study the distribution of the ability of lipoxygenase (LOX) to form GLVs, we found that a liverwort, Marchantia polymorpha, formed n-hexanal and (Z)-3-hexenal. Some LOXs execute a secondary reaction to form short chain volatiles. One of the LOXs from M. polymorpha (MpLOX7) oxygenized arachidonic and α-linolenic acids at almost equivalent efficiency and formed C6-aldehydes during its catalysis; these are likely formed from hydroperoxides of arachidonic and α-linolenic acids, with a cleavage of the bond between carbon at the base of the hydroperoxy group and carbon of double bond, which is energetically unfavorable. These lines of evidence suggest that one of the LOXs in liverwort employs an unprecedented reaction to form C6 aldehydes as by-products of its reaction with fatty acid substrates.

show abstract