A multifunctional surface for blood contact with fibrinolytic activity, ability to promote endothelial cell adhesion and inhibit smooth muscle cell adhesion

Abstract

Blood compatible materials are required for a wide variety of medical devices. Despite many years of intensive effort, however, the blood compatibility problem, in particular the ability to prevent thrombosis, remains unsolved. Based on the knowledge that the vascular endothelium, the ultimate blood contacting surface, draws on several mechanisms to maintain blood fluidity, it seems reasonable that analogous multifunctionality should be the goal for blood compatible biomaterials.In the present work, a polyurethane surface was modified with the terpolymer poly(2-hydroxyethyl methacrylate-co-6amino-2-(2-methacylamido)-hexanoic acid-co-1-adamantan-1-yl methyl methacrylate) (poly(HEMA-co-LysMA-co-AdaMA)), referred to as PU-PHLA. Poly(HEMA) and poly(LysMA) were intended to provide, respectively, resistance to non-specific protein adsorption and the ability to lyse incipient clots. The heparin-like moiety, sulfonated β-cyclodextrin was immobilized on the PU-PHLA via host-guest interactions with the poly(AdaMA). This component is expected to inhibit coagulation and smooth muscle cell proliferation and to promote endothelialization. The resulting materials were shown to have multifunctionalities including fibrinolytic activity, anticoagulant activity and the ability to promote endothelial cell adhesion and inhibit smooth muscle cell adhesion. This work provides a new strategy for the development of multifunctional, endothelial-mimicking, biomaterials for blood contacting applications.

Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization

“…However, due to the incomplete coverage of endothelial cells (ECs) on the surface of vascular substitutes (causing intimal hyperplasia), tissue-engineered grafts for small vessels often cause occlusions (thrombosis) [ 4 ]. Complete endothelialization along the inner wall of a scaffold holds promises in solving this issue [ 5 , 6 ]. Using such a method, the in vitro expanded patient-derived ECs are firstly seeded on the luminal surface of the scaffold, and allowed to form a monolayer of evenly distributed ECs prior to transplantation.…”

Sulfonate Groups and Saccharides as Essential Structural Elements in Heparin-Mimicking Polymers Used as Surface Modifiers: Optimization of Relative Contents for Antithrombogenic Properties

“…Surface endothelialization is a promising approach for the improvement of blood compatibility, and heparin and heparin mimetics have been widely used as modifiers to promote this effect. ,,, It was thus of interest to investigate the behavior of ECs on our PU surfaces grafted with PSS, PMAG, and P­(SS- co -MAG).…”

“…In addition, it has been reported that different surfaces (stainless steel, PU, poly­(ethylene glycol) diacrylate hydrogel scaffolds, silk fibroin scaffolds, etc.) modified with heparin or heparin mimics showed an inhibitory effect on HUVSMC adhesion and proliferation. ,− …”

A highly sensitive and ultra-stretchable zwitterionic liquid hydrogel-based sensor as anti-freezing ionic skin

“…As the content of LysMA increases, the adhesive strength of the hydrogel rst increases to a maximum value of 40.1 kPa and then decreases. This may be attributed to the inner electrostatic interactions of LysMA; 36 high LysMA content likely reinforces the hydrogen bonds between LysMA and decreases the interactions between the molecules and the substrate, resulting in a decrease in the adhesion value. 37 Therefore, optimal LysMA content is essential to enhance the adhesion of the Am-ZIL-LysMA hydrogel.…”