A Multimodal Multi‐Shank Fluorescence Neural Probe for Cell‐Type‐Specific Electrophysiology in Multiple Regions across a Neural Circuit

Abstract

Cell‐type‐specific, activity‐dependent electrophysiology can allow in‐depth analysis of functional connectivity inside complex neural circuits composed of various cell types. To date, optics‐based fluorescence recording devices enable monitoring cell‐type‐specific activities. However, the monitoring is typically limited to a single brain region, and the temporal resolution is significantly low. Herein, a multimodal multi‐shank fluorescence neural probe that allows cell‐type‐specific electrophysiology from multiple deep‐brain regions at a high spatiotemporal resolution is presented. A photodiode and an electrode‐array pair are monolithically integrated on each tip of a minimal‐form‐factor silicon device. Both fluorescence and electrical signals are successfully measured simultaneously in GCaMP6f expressing mice, and the cell type from sorted neural spikes is identified. The probe's capability of combined electro‐optical recordings for cell‐type‐specific electrophysiology at multiple brain regions within a neural circuit is demonstrated. The new experimental paradigm to enable the precise investigation of functional connectivity inside and across complex neural circuits composed of various cell types is expected.

Electrical Stimulation of Human Mesenchymal Stem Cells on Conductive Chitosan-Polyaniline Substrates Promotes Neural Priming

“…Flue 4AM has been used as a membrane-permeable, Ca +2 dependent dye, which increases fluorescence on binding of free Ca +2 [86] . Previous studies reported that ES can induce a change in Ca +2 ion concentration of neuron cells, resulting in the increased fluorescence intensity of Flue 4 AM dye in the neuron [87,88]. As As expected, undifferentiated hMSCs (non-neuronal cells) mostly exhibited nonexcitable behavior and failed to exhibit membrane depolarization after ES treatment.…”

“…Flue 4AM has been used as a membrane-permeable, Ca +2 dependent dye, which increases fluorescence on binding of free Ca +2 [86]. Previous studies reported that ES can induce a change in Ca +2 ion concentration of neuron cells, resulting in the increased fluorescence intensity of Flue 4 AM dye in the neuron [87, 88]. As Figure 7C-D show, after ES, the Fluo-4 fluorescence level of the differentiated hMSCs on both the soft and stiff CS-g-PANI significantly increased compared to cells without electrical stimulation.…”

Neural probe system for behavioral neuropharmacology by bi-directional wireless drug delivery and electrophysiology in socially interacting mice

“…1b). Also, the microelectrodes were electroplated with Pt black to enhance the neural recording performance [17][18][19][20][21] . The longterm stability of the probe, including Pt black microelectrodes, was demonstrated in our previously reported study 21 .…”

“…Also, the microelectrodes were electroplated with Pt black to enhance the neural recording performance [17][18][19][20][21] . The longterm stability of the probe, including Pt black microelectrodes, was demonstrated in our previously reported study 21 . In addition, the microfluidic channels were designed to have low fluidic resistance, which enables the delivery of the drug by low pressure that is generated from a miniaturized, electrolytic pump.…”

Design and simulation of a neural interface based on a microfluidic flexible interconnection cable for chemical delivery

“…Among them, invasive neural interfaces are promising tools because of their ability to collect more sophisticated and direct electrophysiological data [5]. Silicon-based invasive neural interfaces have been widely used, and their performance has been proven in many studies [7][8][9][10]. However, longlasting integration of invasive neural interfaces with the neural tissue is the most challenging issue to be addressed at present [11].…”