A Multimycotoxin Detection Method for Aflatoxins, Ochratoxins, Zearalenone, Sterigmatocystin, and Patulin

Stoloff, Leonard; Nesheim, Stanley; Yin, Lillian; Rodricks, J Y; Stack, Michael E.; Campbell, A. D.

doi:10.1093/jaoac/54.1.91

Cited by 38 publications

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“…After 5 days of incubation, the wheat cultures were removed for toxin extraction. Ninety milliliters of acetonitrile were added to each of the wheat cultures, and the aflatoxins were extracted according to the procedure described by Stoloff et al (16).…”

Section: Methodsmentioning

confidence: 99%

“…Y.) thin-layer chromatography (TLC) plates which were developed in benzene-methanol-acetic acid (18: 1: 1) in unlined developing tanks according to Stoloff et al (16). Blue fluorescing spots corresponding to aflatoxin B1 standards were scribed and scraped from the plates with a vacuum zone collection apparatus (Brinkman Instruments, Westbury, N.…”

Section: Methodsmentioning

confidence: 99%

“…The suspension was centrifuged at 10 C for 10 min at 7,970 x g. The supernatant fluid was decanted and stored at 4 C for further testing. The precipitants were recombined and extracted with 270 ml of acetonitrile (16). The suspension was centrifuged at 7,970 x g and the acetonitrile was decanted into a separatory funnel.…”

Section: Methodsmentioning

confidence: 99%

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Tissue Distribution and Metabolism of Aflatoxin B1-14C in Broiler Chickens

Mabee¹,

Chipley²

1973

Applied Microbiology

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The effects of administering low levels of aflatoxin B,-14C by crop intubation daily for 14 days to broiler chickens were determined. Studies on the distribution of 14C in the blood, selected organs, tissues, and excreta were conducted. No toxic effects were observed in broiler chickens during the 14 days of the experiment. The broiler chickens excreted 90.64% of the 14C administered. Of the 'IC retained, 11.04, 9.83, 4.30, 12.52, 31.66, and 30.63% were detected in the blood, liver, heart, gizzard, breast, and leg, respectively. Chemical assay of those samples demonstrating radioactivity revealed that 81.2% of the radioactivity in these substrates was not extractable by classical extraction procedures while approximately 10% was extractable. Treatment of aqueous extracts for conjugated steroids by treatment with beta-glucuronidase revealed that 31.5% of the 14C detected in the aqueous extract was a liberated glucuronide conjugate of aflatoxin M,-14C.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Tissue Distribution and Metabolism of Aflatoxin B1-14C in Broiler Chickens

Mabee¹,

Chipley²

1973

Applied Microbiology

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“…For patulin, the method of Stoloff et al (14) was used. An analytical standard was kindly supplied by T. Moller, National Food Administration, Uppsala.…”

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confidence: 99%

Isolation of Roquefortine C from Feed Grain

Häggblom

1990

Appl Environ Microbiol

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“…Derartige Stoffe konnen bei nahezu gleichartigen Verhalten bei der dunnschichtchromatischen Auftrennung eines Futtermittelextraktes die Fluoreszenz eines Mykotoxins abschwachen oder verstarken und nicht nur quantitativ, sondern sogar auch qualitativ die Auswertung unter der Voraussetzung erschweren, wenn mit internen und externen Standard gearbeitet worden ist. Diese Feststellung gilt sowohl fur die Aflatoxine als auch fur die anderen Mykotoxine, wenn ihr Nachweis nach dem gleichen Prinzip erfolgt (43, 73, 75, 78, 81,88). Der Nachteil, dai3 auf diese Weise vor allem quantitative Fehlbeurteilungen von bis zu 100 O/o auftreten konnen (29, 32, 38), hat die Aussagekraft der physikalisch-chemischen Verfahren erheblich eingeschrankt und uns veranlafit, nach geeigneten biologischen Testobjekten zu suchen.…”

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Biologischer Nachweis von Mykotoxinen*

Gedek¹

1972

Zentralblatt für Veterinärmedizin Reihe B

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Summary Biological detection of mycotoxins The biological detection of toxic metabolic products of fungi is no less sensitive than physico‐chemical methods. So far as the choice of suitable material for testing is concerned, these biological methods are in fact often superior. The biological techniques are lacking, however in sufficient specificity to enable the presence of a particular mycotoxin to be detected in a food or animal feed. As this statement applies the physico‐chemical methods of detection so far used, it may be best to combine the two types of method in order to reduce the possibilities of error, especially when studying cases of damage (e. g. from feeding mould‐contaminated food to farm livestock). The values for LD50 or LC50 are given for hepatotoxins, aflatoxins, B1, G1, B2, G2, ochratoxin A, sterigmatocystine, diacetoxyscirpenol and rubratoxin B in the test with chick embryos and culture cells. A discription is given of the technique for these procedures, complementary to the usual methods of determining mycotoxins by thin‐layer chromatography. Résumé Mise en évidence biologique des mycotoxines Les procédés de mise en évidence biologiqies des métabolites toxiques des moisissures ne sont pas forcément inférieures aux méthodes physico‐chimiques, quant à leur sensibilité. A condition d'effectuer un choix judicieux des objets à tester, ces méthodes biologiques se révèlent parfois même supérieures. Mais il leur manque cependant une spécificité suffisante, qui permette de certifier la présence d'une certaine mycotoxine dans un aliment ou un fourrage. Comme cette remarque touche également les méthodes de mise en évidence physico‐chimiques employées jusqu'ici, on conseille de combiner les deux groupes de methodes, pour diminuer les possibilités d'erreur, principalement lorsque l'on étudie des cas de dommages (par ex. après administration de fourrages moisis à des animaux de rente). On indique les valeurs (LD50, resp. LC50) pour les hépatotoxines, aflatoxines B1, G1, B2, G2, ochratoxine A, sterigmatocystine, diacétoxyscirpenol et rubratoxine B dans le test sur embryon de poulet et culture de cellules. On décrit la méthodologie et l'on commente l'application de ces procédés comme complément des méthodes usuelles de détermination des mycotoxines par chromatographie sur couche mince. Resumen Identificación biológica de las mycotoxinas Las técnicas biológicas para identificar los productos metabólicos tóxicos de los hongos micelianos no son siempre inferiores, ni con mucho, en cuanto a la sensibilidad, a los métodos físicoquímicos. Bajo la condición previa de la elección correcta de objetos patrones adecuados incluso en ocasiones sobrepasan aquí las primeras a las últimas. Sin embargo, los procedimientos biológicos carecen de la especificidad suficiente, que permita la revelación segura de la presencia de una mycotoxina determinada en un alimento o pienso. Puesto que esto también atañe a las técnicas físicoquímicas de identificación probadas hasta la fecha, se recomienda la aplicación combinada de ambos gru...

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A Multimycotoxin Detection Method for Aflatoxins, Ochratoxins, Zearalenone, Sterigmatocystin, and Patulin

Cited by 38 publications

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Tissue Distribution and Metabolism of Aflatoxin B1-14C in Broiler Chickens

Tissue Distribution and Metabolism of Aflatoxin B1-14C in Broiler Chickens

Isolation of Roquefortine C from Feed Grain

Biologischer Nachweis von Mykotoxinen*

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