A multipathogen selective enrichment broth for simultaneous growth of Salmonella spp., Vibrio parahaemolyticus, and Vibrio cholerae

Abstract

A selective enrichment broth (SVV) was formulated to allow concurrent growth of Salmonella spp., V. parahaemolyticus, and V. cholerae. Potassium tellurite and sodium citrate were added as the inhibitors, while glucose, mannitol, anhydrous sodium sulfi te and sodium pyruvate were employed as the growth-promoters. When mixed in equal or varied proportions, the target pathogens in SVV had a great accumulation (10 5 10 8 CFU/ml) and effectively inhibited the growth of competitive microfl ora. In the artifi cially contaminated samples, a high recovery of these 3 target pathogens was obtained in SVV. Finally, Salmonella spp., V. parahaemolyticus, and V. cholerae were detected from 608 suspicious food samples by SVV with real-time PCR, and no false-positive or -negative results were reported. In summary, SVV has been shown to be a suitable broth for the simultaneous detection of the 3 pathogens by multipathogen detection on a single-assay platform.

A multipathogen selective enrichment broth for simultaneous growth of Salmonella enteria, Escherichia coli O157:H7, and Shigella flexneri

“…Potassium tellurite and lithium chloride had a negative effect on the growth of gram-negative bacteria (Sorensen & Rosen, 1980;Zadik, Chapman, & Siddons, 1993). Our some negative bacteria and no significant influences on the three target pathogens, which were same to previously reported study results (Xiao, Li, Qin, Yu, & Wu, 2010;Yu et al, 2010) (Table 2).…”

“…3 and 0.02 g/L novobiocin sodium had good effect on inhibition of gram‐positive pathogen such as L . monocytogenes and Staphylococcus aureus , while 0.0001 g/L potassium tellurite and 0.5 g/L lithium chloride shows inhibitory effect on some negative bacteria and no significant influences on the three target pathogens, which were same to previously reported study results (Xiao, Li, Qin, Yu, & Wu, ; Yu et al, ) (Table ).…”

“…For example, SEL still supported the growth of Enterobacter aerogenes , Serratia marcescen , Enterobacter cloacae , Pesudomonas aeruginosa , and Hafnia alvei (Kim & Bhunia, ); SVV could not inhibit the growth of Shigella dysenteriae , and V . alginolyticus (Xiao et al, ).…”

Development of a multi-pathogen enrichment broth for simultaneous growth of five common foodborne pathogens

“…In the mix culture experiment, the types of target pathogens in the present research were more than three (only three in previously published studies, Kim and Bhunia, 2008;Xiao et al, 2010Xiao et al, , 2014Yu et al, 2010). It was noteworthy that among all five target pathogens, S. Enteritidis and E. coli O157:H7 exhibited higher growth capabilities, and the maximum number of 10 8 CFU ml -1 was sustained after 24-h enrichment, whether at high or low initial levels.…”

“…Yu et al (2010) elaborated a multi-pathogen enrichment broth for the simultaneous growth of S. enterica, S. aureus and L. monocytogenes. Xiao et al (2010) formulated a multipathogen enrichment broth for the simultaneous growth of Salmonella enterica, Vibrio parahaemolyticus and Vibrio cholerae. Xiao et al (2014) also reported a multipathogen enrichment broth for the simultaneous growth of Salmonella enterica, Staphylococcus aureus and Shigella dysenteriae.…”